Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   121952
Name   oriT_pK190-1-B in_silico
Organism   Enterococcus faecalis strain K190-1
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP116573 (9452..9489 [-], 38 nt)
oriT length   38 nt
IRs (inverted repeats)      7..12, 16..21  (AGTAAC..GTTACT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 38 nt

>oriT_pK190-1-B
ATACGAAGTAACGAAGTTACTGCGTATAAGTGCGCCCT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   13835 GenBank   WP_002332652
Name   mobQ_PML73_RS14825_pK190-1-B insolico UniProt ID   _
Length   661 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 661 a.a.        Molecular weight: 77293.34 Da        Isoelectric Point: 9.3596

>WP_002332652.1 MULTISPECIES: MobQ family relaxase [Enterococcus]
MAIFHLSMTIAKRENGKRSLIAMASYRSGEKLYSELYEKTNLYNHRTVKPEAFILKPDYVPNEFLDRQTL
WNKMELAEKSPNAQLCREVNVALPIELNNSDQRMLIEDFVKDNFVNEGMIADVAIHRDDENNPHAHIMLT
MREVDSEGNILNKSHRIPKLDENGNQIFNEKGQRVTVSIKTNDWGRKSLVSEIRKDWADKVNQYLKDRNI
DQQITEKSHAELGKKELPTIHEGFYSKKLEDKGVISELKRKNLEIQSYNDILAELDKLENQEKVLKQDQN
FTLKFEKTFSPLEKGELKNLSKELKLFINDENIDKRLGELKRWENSLIFNNKMEIQKQRLMLSKISSERD
MLTKANEILDKQAERFFKKSYPSLNIDKFSNHEVRAMVNETIFRKQLLNKDQLAEVIYNERVVEKEESKK
IFKEKPFQTSRYLDSKIKQIEDSITKENNPERKEILSIKKEKLIGIKQGLIEYVQSEVERKFDKNVSIDS
VIEGEMLLAKADYYKTTDFSKVEGVARFSSEEINSMLEQSKGFLTNIQTVKIPNDCQGVFFVQDSMKHID
ELSPLAKQNLKKVVNRNAYLPDSDKIELSKEIENTNKDQSQELDKDVPEKNEVTVKMFQFAKSINRLLSG
NQLQKKRNLDKLIKQTKAKKNQSLQRNIPLR

  Protein domains


Predicted by InterproScan.

Nicking enzyme C-terminal middle helical domain (NES_C_h)

(289-394)

MobA/MobL family (MobA_MobL)

(18-242)


  Protein structure



No available structure.




T4CP

ID   16198 GenBank   WP_032490379
Name   t4cp2_PML73_RS14870_pK190-1-B insolico UniProt ID   _
Length   551 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 551 a.a.        Molecular weight: 63071.11 Da        Isoelectric Point: 4.7399

>WP_032490379.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Lactobacillales]
MTSLLAESDGLILGKFPSGKVVIQPEDSKISNRNIFVVGGPGSFKTQSYVLPNVVNNRSTSIVVTDPKGE
IYELTNEIKKAQGFKTVVINFKDFLLSSRYNPLLYIRKSNDTNKIANVIVSAKNDPKRKDFWFNAQLNLL
NTLIKYVYFEYEPSARTIEGILDFLEEFDPRYNEEGVSELDEQFERLPDGHEAKRSYYLGFRQAQSEARP
NIVISLLTTLQDFVDKEVSEFTSTNDFFFEELGTSKICLYVLISPLDRTWDGLVNLFFQQMFTELYFLGD
KHNAKLPVPLVMLLDEFVNLGYFPTYENFLATCRGYRISVSTILQSLPQGFELYGDKKFKAIIGNHAIKI
CLGGVEETTAEYFSRQVNDTTIKVYTGGTSESKTSAKTTNRSGSKSESYGYQKRRLITEGEVINLQQEEN
GRKSIVLIDGKPYMLRKTPQFELFGNLLKKHEISQQDYISSQTEFAKEYIEELEKQHKQRKVQLASAPIF
HEKKQEDDLNKKEINLPEQPIESEVTEEEEEKELSMSDILSSFDEGIEENTANNDNSELPF

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(33-456)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 11947..25981

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PML73_RS14800 (PML73_14800) 7027..7344 + 318 WP_002326830 hypothetical protein -
PML73_RS14805 (PML73_14805) 7878..8375 + 498 WP_002338758 DnaJ domain-containing protein -
PML73_RS14810 (PML73_14810) 8409..8642 + 234 WP_002332654 hypothetical protein -
PML73_RS14815 (PML73_14815) 8736..8993 - 258 WP_000002668 hypothetical protein -
PML73_RS14820 (PML73_14820) 8996..9295 - 300 WP_002332653 hypothetical protein -
PML73_RS14825 (PML73_14825) 9587..11572 + 1986 WP_002332652 MobQ family relaxase -
PML73_RS14830 (PML73_14830) 11596..11928 + 333 WP_000713503 CagC family type IV secretion system protein -
PML73_RS14835 (PML73_14835) 11947..12330 + 384 WP_002332651 hypothetical protein trsC
PML73_RS14840 (PML73_14840) 12347..12976 + 630 WP_002332650 hypothetical protein trsD
PML73_RS14845 (PML73_14845) 12987..14948 + 1962 WP_002332649 VirB4 family type IV secretion system protein virb4
PML73_RS14850 (PML73_14850) 14962..16314 + 1353 WP_002332648 hypothetical protein trsF
PML73_RS14855 (PML73_14855) 16336..17445 + 1110 WP_002332647 lysozyme family protein orf14
PML73_RS14860 (PML73_14860) 17458..18009 + 552 WP_002332646 hypothetical protein -
PML73_RS14865 (PML73_14865) 18014..18445 + 432 WP_002332645 hypothetical protein -
PML73_RS14870 (PML73_14870) 18438..20093 + 1656 WP_032490379 VirD4-like conjugal transfer protein, CD1115 family -
PML73_RS14875 (PML73_14875) 20111..21037 + 927 WP_271865582 hypothetical protein traP
PML73_RS14880 (PML73_14880) 21034..21966 + 933 WP_104888282 conjugal transfer protein TrbL family protein trsL
PML73_RS14885 (PML73_14885) 21983..22951 + 969 WP_002377195 hypothetical protein -
PML73_RS14890 (PML73_14890) 23000..23236 + 237 Protein_29 GNAT family N-acetyltransferase -
PML73_RS14895 (PML73_14895) 23237..24676 + 1440 WP_001028144 aminoglycoside O-phosphotransferase APH(2'')-Ia -
PML73_RS14900 (PML73_14900) 24768..25136 + 369 WP_000516429 hypothetical protein -
PML73_RS14905 (PML73_14905) 25196..25981 + 786 WP_271865588 LPXTG cell wall anchor domain-containing protein prgC
PML73_RS14910 (PML73_14910) 26287..27141 - 855 WP_032490939 aminoglycoside 6-adenylyltransferase -
PML73_RS14915 (PML73_14915) 27749..28297 + 549 WP_250856472 ATP-binding cassette domain-containing protein -
PML73_RS14920 (PML73_14920) 28598..28696 - 99 WP_142435003 type I toxin-antitoxin system Fst family toxin -
PML73_RS14925 (PML73_14925) 29119..29367 + 249 WP_014862456 helix-turn-helix domain-containing protein -

Host bacterium

ID   22379 GenBank   NZ_CP116573
Plasmid name   pK190-1-B Incompatibility group   -
Plasmid size   38206 bp Coordinate of oriT [Strand]   9452..9489 [-]
Host baterium   Enterococcus faecalis strain K190-1

Cargo genes

Drug resistance gene   erm(B), aac(6')-aph(2'')
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -