Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 121946
• Name: oriT_pK204-1-B
• Organism: Vagococcus lutrae strain K204-1
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP116509 (5271..5311 [+], 41 nt)
• oriT length: 41 nt
• IRs (inverted repeats): 14..22, 29..37 (TAAAGTATA..TATACTTTA) 2..8, 13..19 (ACTTTAT..ATAAAGT)
• Location of nic site: 27..28
• Conserved sequence flanking the nic site: GTGTGTTATA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 41 nt

>oriT_pK204-1-B
CACTTTATGAATATAAAGTATAGTGTGTTATACTTTACATG

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_PML95_RS10190_pK204-1-B

• ID: 13833
• GenBank: WP_231450806
• Name: mobV_PML95_RS10190_pK204-1-B
• UniProt ID: _
• Length: 420 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 420 a.a.
• Molecular weight: 49726.34 Da
• Isoelectric Point: 9.9163

>WP_231450806.1 MULTISPECIES: MobV family relaxase [Enterococcaceae]
MSYAVCRMQKVKSPGLKGMQFHNQRERESRTNEDIDRERTHENYDLQNDKNINYNERVKDIIDSQKTGTR
KTRKDAVLVNELLVTSDRDFFERLDPGEQKRFFEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPM
RDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVK
KDEWTAYSDKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDN
DRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSKISDLKRDVSL
IYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKYDLEPKKNEFELTHNREVKKERSRDQGMSL

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-193)

Protein structure

No available structure.

Host bacterium

• ID: 22373
• GenBank: NZ_CP116509
• Plasmid name: pK204-1-B
• Incompatibility group: -
• Plasmid size: 27307 bp
• Coordinate of oriT [Strand]: 5271..5311 [+]
• Host baterium: Vagococcus lutrae strain K204-1

Cargo genes

• Drug resistance gene: erm(C), fexA, optrA
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -