Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 121760 |
| Name | oriT_pFYY063-optrA-70K  |
| Organism | Enterococcus casseliflavus strain CQFYY22-063 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP116030 (34338..34367 [-], 30 nt) |
| oriT length | 30 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 30 nt
>oriT_pFYY063-optrA-70K
AACGTAAGTTAGTGGGTATAAGTGCGCCCT
AACGTAAGTTAGTGGGTATAAGTGCGCCCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 13730 | GenBank | WP_233701194 |
| Name | mobQ_PEZ80_RS16655_pFYY063-optrA-70K |
UniProt ID | _ |
| Length | 661 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 661 a.a. Molecular weight: 77313.36 Da Isoelectric Point: 9.2532
>WP_233701194.1 MULTISPECIES: MobQ family relaxase [Lactobacillales]
MAIFHLSMTIAKREGGKRSLIAMASYRSGEKLYSELYEKTNLYNHRTVKPEAFILKPDYVPNEFLDRQTL
WNKMELAEKSPNAQLCREVNVALPIELNNSDQRMLIEDFVKDNFVSEGMIADVAIHRDDENNPHAHIMLT
MREVDSEGNILNKRKRIPKLDENGNQIFNEKGQRVTVSIKTNDWDRKSLVSEIRKDWADKVNQYLKDRNI
DQQITEKSHAELGKKELPTIHEGFYSKKLEDKGVISELKRKNLEIQSYNDILAELDKLESQDKVLKQDQN
FTLKFEKTFSPLEKGELKNLSKELKLFINDENIDKRIGELKRWENSLIFNNKMEIQKQRLMLSKISSERD
MLTKANEILDKQAERFFKKSYPSLNIDKFSNHEVRAMVNETIFRKQLLNKDQLAEVIYNERVIEKEESKK
IFKEKPFQTSRYLDSKIKQVEDSITKENNPERKEILSIKKEKLIGIKQGLIEYVQSEVERKFDKNVSIDS
VIEGEMLLAKADYYKTTDFSKVEGVARFSSEEINSMLEQSKGFLTNIQTVKIPNDCQGVFFVQDSMKYID
ELSPLAKQNLKKVVNRNAYLPDSDKIELSKEIENTNKDQSQELDKDVPEKNEVTVKMFQFAKSINRLLSG
NQLQKKRNLDKLIKQTKAKENQSLQRNIPLR
MAIFHLSMTIAKREGGKRSLIAMASYRSGEKLYSELYEKTNLYNHRTVKPEAFILKPDYVPNEFLDRQTL
WNKMELAEKSPNAQLCREVNVALPIELNNSDQRMLIEDFVKDNFVSEGMIADVAIHRDDENNPHAHIMLT
MREVDSEGNILNKRKRIPKLDENGNQIFNEKGQRVTVSIKTNDWDRKSLVSEIRKDWADKVNQYLKDRNI
DQQITEKSHAELGKKELPTIHEGFYSKKLEDKGVISELKRKNLEIQSYNDILAELDKLESQDKVLKQDQN
FTLKFEKTFSPLEKGELKNLSKELKLFINDENIDKRIGELKRWENSLIFNNKMEIQKQRLMLSKISSERD
MLTKANEILDKQAERFFKKSYPSLNIDKFSNHEVRAMVNETIFRKQLLNKDQLAEVIYNERVIEKEESKK
IFKEKPFQTSRYLDSKIKQVEDSITKENNPERKEILSIKKEKLIGIKQGLIEYVQSEVERKFDKNVSIDS
VIEGEMLLAKADYYKTTDFSKVEGVARFSSEEINSMLEQSKGFLTNIQTVKIPNDCQGVFFVQDSMKYID
ELSPLAKQNLKKVVNRNAYLPDSDKIELSKEIENTNKDQSQELDKDVPEKNEVTVKMFQFAKSINRLLSG
NQLQKKRNLDKLIKQTKAKENQSLQRNIPLR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 16041 | GenBank | WP_271292755 |
| Name | t4cp2_PEZ80_RS16700_pFYY063-optrA-70K |
UniProt ID | _ |
| Length | 551 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 551 a.a. Molecular weight: 63194.27 Da Isoelectric Point: 4.7731
>WP_271292755.1 VirD4-like conjugal transfer protein, CD1115 family [Enterococcus casseliflavus]
MSSLLAESDGLILGKFQSGNVVIQPEDSKISNRNIFVVGGPGSFKTQSYVLPNVVNNRSTSIVVTDPKGE
IYELTNEIKKAQGFKTVVINFKDFLLSSRYNPLLYIRKSNDTNKIANVIVSAKNDPKRKDFWFNAQLSLL
NTLIKYVYFEYEPSSRTIEGILDFLEEFDPRYNEEGVSELDEQFERLPDGHEAKRSYYLGFRQAQSEARP
NIVISLLTTLQDFVDKEVSEFTSTNDFFFEELGTSKICLYVLISPLDRTWDGLVNLFFQQMFTELYFLGD
KHNAKLPVPLVMLLDEFVNLGYFPTYENFLATCRGYRISVSTILQSLPQGFELYGDKKFKAIIGNHAIKI
CLGGVEETTAEYFSRQVNDTTIKVYTGGTSESKTSAKTTNRSGSKSESYGYQKRRLITEGEVINLQQEEN
GRKSIVLIDGKPYMLRKTPQFELFGNLLKKHEISQQYYISSQTEFAKEYIEELEKQHKQRKVQLASAPIF
HEKKQEDDLNKKEINLPEQPIESEVTEEEEEKELLMSDILSSFDEGIEENKTNNDNSELPF
MSSLLAESDGLILGKFQSGNVVIQPEDSKISNRNIFVVGGPGSFKTQSYVLPNVVNNRSTSIVVTDPKGE
IYELTNEIKKAQGFKTVVINFKDFLLSSRYNPLLYIRKSNDTNKIANVIVSAKNDPKRKDFWFNAQLSLL
NTLIKYVYFEYEPSSRTIEGILDFLEEFDPRYNEEGVSELDEQFERLPDGHEAKRSYYLGFRQAQSEARP
NIVISLLTTLQDFVDKEVSEFTSTNDFFFEELGTSKICLYVLISPLDRTWDGLVNLFFQQMFTELYFLGD
KHNAKLPVPLVMLLDEFVNLGYFPTYENFLATCRGYRISVSTILQSLPQGFELYGDKKFKAIIGNHAIKI
CLGGVEETTAEYFSRQVNDTTIKVYTGGTSESKTSAKTTNRSGSKSESYGYQKRRLITEGEVINLQQEEN
GRKSIVLIDGKPYMLRKTPQFELFGNLLKKHEISQQYYISSQTEFAKEYIEELEKQHKQRKVQLASAPIF
HEKKQEDDLNKKEINLPEQPIESEVTEEEEEKELLMSDILSSFDEGIEENKTNNDNSELPF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 36833..46852
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| PEZ80_RS16635 (PEZ80_16630) | 32664..33344 | + | 681 | WP_001015311 | IS6-like element IS1216 family transposase | - |
| PEZ80_RS16640 (PEZ80_16635) | 33416..33592 | + | 177 | WP_181728217 | hypothetical protein | - |
| PEZ80_RS16645 (PEZ80_16640) | 33622..33879 | - | 258 | WP_010717195 | hypothetical protein | - |
| PEZ80_RS16650 (PEZ80_16645) | 33882..34181 | - | 300 | WP_002325624 | hypothetical protein | - |
| PEZ80_RS16655 (PEZ80_16650) | 34473..36458 | + | 1986 | WP_233701194 | MobQ family relaxase | - |
| PEZ80_RS16660 (PEZ80_16655) | 36482..36814 | + | 333 | WP_000713503 | CagC family type IV secretion system protein | - |
| PEZ80_RS16665 (PEZ80_16660) | 36833..37216 | + | 384 | WP_001083514 | glycosyl transferase | trsC |
| PEZ80_RS16670 (PEZ80_16665) | 37233..37862 | + | 630 | WP_104888277 | hypothetical protein | trsD |
| PEZ80_RS16675 (PEZ80_16670) | 37873..39834 | + | 1962 | WP_231428364 | VirB4 family type IV secretion system protein | virb4 |
| PEZ80_RS16680 (PEZ80_16675) | 39848..41200 | + | 1353 | WP_271292754 | conjugal transfer protein TraF | trsF |
| PEZ80_RS16685 (PEZ80_16680) | 41222..42331 | + | 1110 | WP_000501303 | lysozyme family protein | orf14 |
| PEZ80_RS16690 (PEZ80_16685) | 42344..42895 | + | 552 | WP_141062720 | hypothetical protein | - |
| PEZ80_RS16695 (PEZ80_16690) | 42900..43331 | + | 432 | WP_163327513 | hypothetical protein | - |
| PEZ80_RS16700 (PEZ80_16695) | 43324..44979 | + | 1656 | WP_271292755 | VirD4-like conjugal transfer protein, CD1115 family | - |
| PEZ80_RS16705 (PEZ80_16700) | 44997..45923 | + | 927 | WP_271292756 | hypothetical protein | traP |
| PEZ80_RS16710 (PEZ80_16705) | 45920..46852 | + | 933 | WP_194849630 | conjugal transfer protein TrbL family protein | trsL |
| PEZ80_RS16715 (PEZ80_16710) | 46869..47837 | + | 969 | WP_194849629 | hypothetical protein | - |
| PEZ80_RS16720 (PEZ80_16715) | 47866..48219 | + | 354 | WP_002339801 | hypothetical protein | - |
| PEZ80_RS16725 (PEZ80_16720) | 48279..48656 | - | 378 | WP_070415974 | hypothetical protein | - |
| PEZ80_RS16730 (PEZ80_16725) | 48903..49148 | + | 246 | WP_047927842 | hypothetical protein | - |
| PEZ80_RS16735 (PEZ80_16730) | 49643..51190 | + | 1548 | WP_194849628 | type I restriction-modification system subunit M | - |
Host bacterium
| ID | 22187 | GenBank | NZ_CP116030 |
| Plasmid name | pFYY063-optrA-70K | Incompatibility group | - |
| Plasmid size | 70094 bp | Coordinate of oriT [Strand] | 34338..34367 [-] |
| Host baterium | Enterococcus casseliflavus strain CQFYY22-063 |
Cargo genes
| Drug resistance gene | fexA, optrA, erm(A) |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |