Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   121739
Name   oriT_pQ15_94_1 in_silico
Organism   Agrobacterium tumefaciens strain Q15/94
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP049218 (208310..208338 [-], 29 nt)
oriT length   29 nt
IRs (inverted repeats)      14..19, 24..29  (CGTCGC..GCGACG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 29 nt

>oriT_pQ15_94_1
AGGGCGCAATATACGTCGCTGGCGCGACG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   13719 GenBank   WP_333722469
Name   traA_G6M86_RS25045_pQ15_94_1 insolico UniProt ID   _
Length   1542 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1542 a.a.        Molecular weight: 171443.47 Da        Isoelectric Point: 9.7581

>WP_333722469.1 Ti-type conjugative transfer relaxase TraA [Agrobacterium tumefaciens]
MAIMFVRAQVISRGAGRSIVSAAAYRHRARMMDEQAGTSFSYRGGGAELKHEELALPDQVPAWLRAAIDG
KSVVAASEALWNAVDAFETRADAQLARELIIALPEELTRSEKIALVREFVRDNLTSKGMIADWVYHDRQG
NPHIHLMTALRPLTEQGFGPKKVPVLGADGEPLRVVTPDRPKGKIVYRLWAGDKETMKAWKIAWADTANR
HLALAGHEIRLDGRSYAEQGLDGIAQKHLGPEKAALARKGVAMHFAPADLARRQEMADRLLADPYLLLKQ
LGNERSTFDERDIAKALHRYVDDPADFANIRAKLMASNDLVLLKPQQANLQTGKVAEPAVFTTRDILRTE
YDMAQSAEVLARRKGFGVAAAKIAAAVRTIETANPEDPFTLDREQVEAVHHVAGDTGIAAVVGHAGAGKS
TLLAAARIAWESGNYRVFGAALAGKAAEGLEDSSGIRSRTLASWELAWENGRDLLDRRDIFVIDEAGMVS
SQQMARVLKRAEEAGAKVVLVGDAMQLQPIQAGAAFRAISERIGSAELAGVRRQREEWAREASRLFARGK
VEEGLDAYAQRGHIVEAESRAEAVERIVADWTSARRDLIQQYADKEQSVRMRGDELLVLAHTNDDVRRLN
EALRNVMKDEGALTVSREFQTERGVREFAVGDRIIFLQNARFLEPRAQKLGPQYVKNGMLGTVVYTGDTH
GDPQLSVRLDNGRDVVISEDSYRNVDHGYAATIHKSQGATVDRTFVLATSMMDQHLTYVSMTRHRDRADL
YAAREDFVPKPEWGKRSLVDHAAGVTGELVGTGNAKFRPQDEDADKSPYADVKTDDGSVHRLWGVSLPKA
LEEAGVSEGDTVTLRKDGVERVTVMVPVIDEKTGKKRLEERTVHRNVWTAKRIETTEVRRQRLEEESHRP
ELFKQLVERLGRSGAKTTTLDFESEAGYRAHARDFAQRRGIDTLSGVAAWMEDGAARQLAWLAQKREQVA
KLWERASVALGFAIESEKRVSYSEERAETRTAKIPTDRQYLIPPKTLFSRSVGEDARRAQLRSDHWKERD
AILRPVLQKIYRDPEGALARLNALASDARIAPRELADDLEASPQRLGRLRGSNLLVDGRAARSERDAAVS
ALSELLPLARSHATEFRRQVERFGMREEQRRAHMSLSIPALSKPAMARLAEIEAVRERGGKDAYKTAFAY
AAEDRLFVQEVRGVNDALNARFGWSAFTDKADTVAQRNMIERMPDDLDLDRREKLNRLFAAIRRFAEEQH
LAERKDRSRIVTGASVLPGKETVPVLPMLAAVTEFETPIDHEARERARAVPHYSQNRAALANAAARIWRD
PAGAVGKIEDLILKGFAGERIAAAISNDPAAYGALRGSGRIMDKLLAVGQERKEAFRAVPEAASRVRLLA
TSFRAAFDAEREAITEERQRMSIAIPGLSPSAEDALRELSVAMKKKNGRLDVAAGSLDPAISKEFATVSR
ALDERFGRNAILRGELDVINRVPPAQRRAFEVMREQLKVLQQVVRMQASQNIVAERHRRVLDRARGVILF
NQ

  Protein domains


Predicted by InterproScan.

MobA/MobL family (MobA_MobL)

(17-249)

BID domain of Bartonella effector protein (Bep) (Bep_C_terminal)

(1054-1125)

BID domain of Bartonella effector protein (Bep) (Bep_C_terminal)

(1308-1397)

AAA domain (AAA_30)

(390-578)


  Protein structure



No available structure.




T4CP

ID   16032 GenBank   WP_333722472
Name   traG_G6M86_RS25060_pQ15_94_1 insolico UniProt ID   _
Length   639 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 639 a.a.        Molecular weight: 70706.70 Da        Isoelectric Point: 9.1521

>WP_333722472.1 Ti-type conjugative transfer system protein TraG [Agrobacterium tumefaciens]
MKGKAKPHPSLLLIIVPIVVTGFTFYVAHWRWPELAAGITGKPQYWFLRASPLPVLLFGPLAGLLAVWAL
PLHRRRPVALASFLCFLLMAGFYGLREFGRLQPLVESGVLTWDRALSFVDMIAVIGAAMGFMAAAVAARI
SSVVPEQVTRARRGTFGDADWLPMSAAARLFPDDGEIVIGERYRVDKELVHELPFDPNDPATWGRGGKAP
LLTYSQDFDSTHMLFFAGSGGFKTTSNVVPTALRYTGPLICLDPSTEVAPMVVDHRRDKLNREVVVLDPA
NPVMGFNVLDGIEHSLKKEEDIVGIAHMLLSESVRFESSTGSYFQSQAHNLLTGLLAHVMLSPEYAGRRN
LRSLRQIVSEPETSVLAMLRDVQEHSTSAFIRETLGVFVNMTEQTFSGVYSTASKDTQWLSLDNYAALVC
GNTFKSSEIAGGKKDVFINIPASILRSYPGIGRVIIGSLINAMVEADGAFTRRALFILDEVDLLGYMRVL
EEARDRGRKYGVSMMLMYQSVGQLERHFGKDGAVSWIDGCAFASYAAIKALDTARNVSAQCGEMTVEVKG
SSRNLGWGTKNSASRKSENINFQRRPLIMPHEITQSMRKDEQIIIVQGHSPIRCGRAIYFRRKDMDMHAR
PNRFAKLGP

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(166-633)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 139422..149027

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6M86_RS24680 (G6M86_24650) 134460..135362 - 903 WP_202799420 DMT family transporter -
G6M86_RS24685 (G6M86_24655) 135544..135969 - 426 WP_003517231 GreA/GreB family elongation factor -
G6M86_RS24690 (G6M86_24660) 136487..136900 + 414 WP_003517236 nucleoside diphosphate kinase regulator -
G6M86_RS24695 (G6M86_24665) 137314..138273 - 960 WP_035200917 helix-turn-helix domain-containing protein -
G6M86_RS24700 (G6M86_24670) 138804..139079 + 276 WP_003517240 helix-turn-helix transcriptional regulator -
G6M86_RS24705 (G6M86_24675) 139422..140432 - 1011 WP_309510146 P-type DNA transfer ATPase VirB11 virB11
G6M86_RS24710 (G6M86_24680) 140441..141613 - 1173 WP_153793587 type IV secretion system protein VirB10 virB10
G6M86_RS24715 (G6M86_24685) 141628..142479 - 852 WP_026330855 P-type conjugative transfer protein VirB9 virB9
G6M86_RS24720 (G6M86_24690) 142479..143150 - 672 WP_003517248 virB8 family protein virB8
G6M86_RS24725 (G6M86_24695) 143154..143420 - 267 WP_025591470 hypothetical protein -
G6M86_RS24730 (G6M86_24700) 143456..144391 - 936 WP_013637412 type IV secretion system protein virB6
G6M86_RS24735 (G6M86_24705) 144396..144629 - 234 WP_174041433 EexN family lipoprotein -
G6M86_RS24740 (G6M86_24710) 144626..145324 - 699 WP_112153474 P-type DNA transfer protein VirB5 virB5
G6M86_RS24745 (G6M86_24715) 145324..147705 - 2382 WP_013637409 VirB4 family type IV secretion/conjugal transfer ATPase virb4
G6M86_RS24750 (G6M86_24720) 147698..148039 - 342 WP_013637408 type IV secretion system protein VirB3 virB3
G6M86_RS24755 (G6M86_24725) 148042..148341 - 300 WP_013637407 TrbC/VirB2 family protein virB2
G6M86_RS24760 (G6M86_24730) 148338..149027 - 690 WP_013637406 type IV secretion protein AvhB1 virB1
G6M86_RS24765 (G6M86_24735) 149029..149568 - 540 WP_013637405 hypothetical protein -
G6M86_RS24770 (G6M86_24740) 149662..150024 + 363 WP_035252472 transcriptional regulator -
G6M86_RS24775 (G6M86_24745) 150368..150772 + 405 WP_003517267 host attachment protein -
G6M86_RS24780 (G6M86_24750) 151632..152021 + 390 WP_236761979 hypothetical protein -
G6M86_RS24785 (G6M86_24755) 152184..152930 - 747 WP_236772274 SDR family oxidoreductase -
G6M86_RS24790 (G6M86_24760) 152985..153419 - 435 WP_010974414 MarR family winged helix-turn-helix transcriptional regulator -

Host bacterium

ID   22166 GenBank   NZ_CP049218
Plasmid name   pQ15_94_1 Incompatibility group   -
Plasmid size   488273 bp Coordinate of oriT [Strand]   208310..208338 [-]
Host baterium   Agrobacterium tumefaciens strain Q15/94

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -