Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   121732
Name   oriT_pCRE2_1 in_silico
Organism   Klebsiella aerogenes strain S2_CRE2
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP074447 (28493..28593 [+], 101 nt)
oriT length   101 nt
IRs (inverted repeats)      80..85, 91..96  (AAAAAA..TTTTTT)
 20..26, 38..44  (TAAATCA..TGATTTA)
Location of nic site      62..63
Conserved sequence flanking the
  nic site    		  GGTGTATAGC
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 101 nt

>oriT_pCRE2_1
TATTTATTTTTTTATCTTTTAAATCAGTACGATAGCGTGATTTATCGCGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   13714 GenBank   WP_012561142
Name   mobF_KHV89_RS24285_pCRE2_1 insolico UniProt ID   A0A1S6KKJ9
Length   1078 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 1078 a.a.        Molecular weight: 120195.37 Da        Isoelectric Point: 6.5230

>WP_012561142.1 MULTISPECIES: MobF family relaxase [Enterobacterales]
MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLVGEIDTFTHMQ
RHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREAEKLAQARTTRQGKSVTQNTN
NLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALKNDELMRNKMHLGDVYKQELALELTKAGYEL
RYNSKNNTFDMAHFSDEQIRAFSRRSEQIEKGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQE
WASRAKTLGIDFDNREWQGHGKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIA
NKQVLGHATIADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKKEQMRAVTEIV
TSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALEDDGLPARTVAAFLKAKDKKLD
EKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQTKAVEAGKPFEQLIKADMQTSYMKDIQRQK
NEVLLEAVKYAAEGNAARALKNITGVNELKEEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDY
IRGNLGLAGTGETFTLLDRVDSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVES
SSGEQIAFSPRTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTDDKKSLASSVS
RDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFNQKPDEHNMTTGTDYQPVSNA
EDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQWSAQEYADYEHYAEASDYDFDSSIYDDYAM
PQTSQAEQSHTGKEHTHEHEHEEGGHEI

  Protein domains


Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C_2)

(851-895)

AAA domain (AAA_30)

(477-653)

TrwC relaxase (TrwC)

(16-287)


  Protein structure


Source ID Structure
AlphaFold DB A0A1S6KKJ9


Auxiliary protein

ID   7551 GenBank   WP_001749975
Name   WP_001749975_pCRE2_1 insolico UniProt ID   A0A5C2CVS6
Length   138 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 138 a.a.        Molecular weight: 15332.60 Da        Isoelectric Point: 5.9670

>WP_001749975.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MPIITAKVSDELLAYIDLVSGGNRSDYLRRCIEAGPGDRESGLKIVADRLSDVNRKLDYLFDRASDADFG
PLRDELKAITETLSGVKFPPAGQMMLHESLAIETLILLRSIAEPGKTKAAKAEVERNGYKVWEPKKER

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A5C2CVS6


T4CP

ID   16025 GenBank   WP_000342688
Name   t4cp2_KHV89_RS24290_pCRE2_1 insolico UniProt ID   _
Length   509 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 509 a.a.        Molecular weight: 57762.87 Da        Isoelectric Point: 9.6551

>WP_000342688.1 MULTISPECIES: type IV secretion system DNA-binding domain-containing protein [Enterobacterales]
MDDRERGLAFLFAITLPPVMVWFLVAKFTYGIDPSTAKYLIPYLVKNTFSLWPLWSALIAGWFIGVGGLI
AFIIYDKSRVFKGERFKKIYRGTELVRARTLADKTRERGVNQLTVANIPIPTYAENLHFSIAGTTGTGKT
TIFNELLFKSIIRGGKNIALDPNGGFLKNFYRPGDVILNAYDKRTEGWVFFNEIRRSYDYERLVNSIVQE
SPDMATEEWFGYGRLIFSEVSKKLHSLYSTVTMEEVIHWACNVDQKKLKEFLMGTPAEAIFSGSEKAVGS
ARFVLSKNLAPHLKMPEGNFSLRDWLDDGKPGTLFITWQEEMKRSLNPLISCWLDSIFSIVLGMGEKESR
INVFIDELESLQFLPNLNDALTKGRKSGLCVYAGYQTYSQLVKVYGRDMAQTILANMRSNIVLGGSRLGD
ETLDQMSRSLGEIEGEVERKESDPQKPWIVRKRRDVKVVRAVTPTEISMLPNLTGYLALPGDMPVAKFKA
KHVKYHRKNPVPGIELREI

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(113-489)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 10062..27926

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHV89_RS24155 (KHV89_24105) 5907..7340 - 1434 WP_001288432 DNA cytosine methyltransferase -
KHV89_RS24160 (KHV89_24110) 7439..7711 + 273 Protein_5 IS1 family transposase -
KHV89_RS24165 (KHV89_24115) 7722..7928 - 207 WP_001749967 hypothetical protein -
KHV89_RS24170 (KHV89_24120) 7933..8574 - 642 WP_012561158 restriction endonuclease -
KHV89_RS24175 (KHV89_24125) 8630..8941 - 312 WP_013279382 hypothetical protein -
KHV89_RS24180 (KHV89_24130) 8977..9291 - 315 WP_016338363 TrbM/KikA/MpfK family conjugal transfer protein -
KHV89_RS24185 (KHV89_24135) 9288..9632 - 345 WP_012561155 hypothetical protein -
KHV89_RS24190 (KHV89_24140) 9648..9953 - 306 WP_012561154 H-NS family nucleoid-associated regulatory protein -
KHV89_RS24195 (KHV89_24145) 10062..10799 + 738 WP_013279384 lytic transglycosylase domain-containing protein virB1
KHV89_RS24200 (KHV89_24150) 10808..11089 + 282 WP_016338364 transcriptional repressor KorA -
KHV89_RS24205 (KHV89_24155) 11099..11392 + 294 WP_001749962 hypothetical protein virB2
KHV89_RS24210 (KHV89_24160) 11442..11759 + 318 WP_000496058 VirB3 family type IV secretion system protein virB3
KHV89_RS24215 (KHV89_24165) 11759..14359 + 2601 WP_012561149 VirB4 family type IV secretion/conjugal transfer ATPase virb4
KHV89_RS24220 (KHV89_24170) 14377..15090 + 714 WP_001749960 type IV secretion system protein virB5
KHV89_RS24225 (KHV89_24175) 15098..15325 + 228 WP_001749959 IncN-type entry exclusion lipoprotein EexN -
KHV89_RS24230 (KHV89_24180) 15341..16381 + 1041 WP_001749958 type IV secretion system protein virB6
KHV89_RS24400 16482..16610 + 129 WP_071882546 conjugal transfer protein TraN -
KHV89_RS24235 (KHV89_24185) 16600..17298 + 699 WP_000646594 virB8 family protein virB8
KHV89_RS24240 (KHV89_24190) 17309..18193 + 885 WP_000735066 TrbG/VirB9 family P-type conjugative transfer protein virB9
KHV89_RS24245 (KHV89_24195) 18193..19353 + 1161 WP_012561145 type IV secretion system protein VirB10 virB10
KHV89_RS24250 (KHV89_24200) 19395..20390 + 996 WP_012561144 ATPase, T2SS/T4P/T4SS family virB11
KHV89_RS24255 (KHV89_24205) 20390..20923 + 534 WP_000792636 phospholipase D family protein -
KHV89_RS24260 (KHV89_24210) 21096..21410 - 315 WP_000091613 hypothetical protein -
KHV89_RS24265 (KHV89_24215) 21665..22021 - 357 WP_000215515 cupin domain-containing protein -
KHV89_RS24270 (KHV89_24220) 22011..22412 - 402 WP_001293886 DUF86 domain-containing protein -
KHV89_RS24275 (KHV89_24225) 22409..22699 - 291 WP_001247892 nucleotidyltransferase family protein -
KHV89_RS24280 (KHV89_24230) 22763..23161 - 399 WP_012561143 DUF6710 family protein -
KHV89_RS24285 (KHV89_24235) 23161..26397 - 3237 WP_012561142 MobF family relaxase -
KHV89_RS24290 (KHV89_24240) 26397..27926 - 1530 WP_000342688 type IV secretion system DNA-binding domain-containing protein virb4
KHV89_RS24295 (KHV89_24245) 27928..28344 - 417 WP_001749975 hypothetical protein -
KHV89_RS24300 (KHV89_24250) 28901..29287 + 387 WP_013279398 plasmid stabilization protein StbA -
KHV89_RS24305 (KHV89_24255) 29296..30012 + 717 WP_012561139 StbB family protein -
KHV89_RS24310 (KHV89_24260) 30014..30382 + 369 WP_012561138 plasmid stabilization protein StbC -
KHV89_RS24315 (KHV89_24265) 30583..30927 + 345 WP_012561137 hypothetical protein -
KHV89_RS24320 (KHV89_24270) 31389..31568 - 180 WP_012561134 protein CcgAI -
KHV89_RS24325 (KHV89_24275) 32397..32901 - 505 Protein_39 antirestriction protein ArdA -

Host bacterium

ID   22159 GenBank   NZ_CP074447
Plasmid name   pCRE2_1 Incompatibility group   IncN
Plasmid size   43178 bp Coordinate of oriT [Strand]   28493..28593 [+]
Host baterium   Klebsiella aerogenes strain S2_CRE2

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -