Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 121727 |
| Name | oriT_pCRE18_1  |
| Organism | Enterobacter kobei strain S18_CRE18 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP075346 (51287..51386 [+], 100 nt) |
| oriT length | 100 nt |
| IRs (inverted repeats) | 25..32, 35..42 (TAACCTGT..ACAGGTTA) |
| Location of nic site | 61..62 |
| Conserved sequence flanking the nic site |
TCCTGCCCGG |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 100 nt
>oriT_pCRE18_1
GAATAAGGGGAGAGTAAAGTTTGATAACCTGTTTACAGGTTAGCTAACTTTACATGTCCTGCCCGGCTTTCGCCGGTGTTTACGCCAAGGAAAGTCTACA
GAATAAGGGGAGAGTAAAGTTTGATAACCTGTTTACAGGTTAGCTAACTTTACATGTCCTGCCCGGCTTTCGCCGGTGTTTACGCCAAGGAAAGTCTACA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 13711 | GenBank | WP_270094261 |
| Name | traI_KJE18_RS23210_pCRE18_1 |
UniProt ID | _ |
| Length | 877 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 877 a.a. Molecular weight: 97552.71 Da Isoelectric Point: 10.5332
>WP_270094261.1 TraI/MobA(P) family conjugative relaxase [Enterobacter kobei]
MISRHIPMRNVKKSSFVELAKYLQDPQSKQERVGVIKVTNCHQENILDAARYDIEPTQRRNTRSEGDKTY
HLVISFRPGEKPSQEVLDAAEERICAAMGYADHQRVSAVHYDTDNVHIHIAINKIHPQTHNIHTPYNDYK
TRSAVCLALEKEFGLESDNHRASKTRSENNAADMERHSGRESLLGWIKRECAEQLKSAANWQELHQTMRQ
NGLELRQKGNGFVITGPDGLAVKASSVDRSLSRAALEKKLGSFESSIPDTKPSRPSPEAIKQRFGSAAHV
KRPGDRPPPMRENRQVHLGDVPVMQISSGKRYEGKPLGSQGASTSALYGRYQSEQQRLTAARNEALRLAK
VKRDRLIENAKRSGRLKRAGIKLLKGPGVNKRYLYGLASKALGEELERANAIYKKDRQAAYDIHKRRTWA
DWLQFKAAGGDDEALKTLRQRNAQQARKEANILHGDKVKKDGRIPGLKPDSVTKEGTIIYRVGESAIRDG
GKRLDVSRDAGDDGLHAALLMAKHRYGDKLTVNGSFEFKKRIVAVAAARELDVSFADPELEKLRQILTKK
AAREREYIHIPFEQRGKARQAGAMWDTEKKSWFVGPYATRDRIENFIRMNEEKDNEQRRQRYAGTERTDA
GRERSGNGNATGANEPGVTGRSTSADAGNGYGASSSGRRTGSGDAVTTAGSQPGRSDDTRTGRSRAAEPN
GERGHSKPNVARPGRQPPPTAKNRLRAMSELGMVRIAGGSEVLLQGNVSDQLEHKTAERADQLRRDMAGA
GGITTPAEVKKPLSSAEKYINEREQKRLKGFDIPKHRGYNSIDKGLFEYAGQRKSGLETLALLKRDDEIV
VMPVSDYTARRLSNLKVGESVTLTPSGSVRTTKGRSR
MISRHIPMRNVKKSSFVELAKYLQDPQSKQERVGVIKVTNCHQENILDAARYDIEPTQRRNTRSEGDKTY
HLVISFRPGEKPSQEVLDAAEERICAAMGYADHQRVSAVHYDTDNVHIHIAINKIHPQTHNIHTPYNDYK
TRSAVCLALEKEFGLESDNHRASKTRSENNAADMERHSGRESLLGWIKRECAEQLKSAANWQELHQTMRQ
NGLELRQKGNGFVITGPDGLAVKASSVDRSLSRAALEKKLGSFESSIPDTKPSRPSPEAIKQRFGSAAHV
KRPGDRPPPMRENRQVHLGDVPVMQISSGKRYEGKPLGSQGASTSALYGRYQSEQQRLTAARNEALRLAK
VKRDRLIENAKRSGRLKRAGIKLLKGPGVNKRYLYGLASKALGEELERANAIYKKDRQAAYDIHKRRTWA
DWLQFKAAGGDDEALKTLRQRNAQQARKEANILHGDKVKKDGRIPGLKPDSVTKEGTIIYRVGESAIRDG
GKRLDVSRDAGDDGLHAALLMAKHRYGDKLTVNGSFEFKKRIVAVAAARELDVSFADPELEKLRQILTKK
AAREREYIHIPFEQRGKARQAGAMWDTEKKSWFVGPYATRDRIENFIRMNEEKDNEQRRQRYAGTERTDA
GRERSGNGNATGANEPGVTGRSTSADAGNGYGASSSGRRTGSGDAVTTAGSQPGRSDDTRTGRSRAAEPN
GERGHSKPNVARPGRQPPPTAKNRLRAMSELGMVRIAGGSEVLLQGNVSDQLEHKTAERADQLRRDMAGA
GGITTPAEVKKPLSSAEKYINEREQKRLKGFDIPKHRGYNSIDKGLFEYAGQRKSGLETLALLKRDDEIV
VMPVSDYTARRLSNLKVGESVTLTPSGSVRTTKGRSR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 16022 | GenBank | WP_004196203 |
| Name | t4cp2_KJE18_RS23200_pCRE18_1 |
UniProt ID | _ |
| Length | 648 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 648 a.a. Molecular weight: 71683.77 Da Isoelectric Point: 8.3842
>WP_004196203.1 MULTISPECIES: type IV secretory system conjugative DNA transfer family protein [Enterobacterales]
MRKPNNAVGPQVRNKKKTARGKLIPAMAITSLAAGIQAATQYFAYSFNYQELLGPNFNHIYAPWSYFKWY
SAWHDRLPDAFFAAGSVGATVAAGGLVLTALTSMMKANSSKANEYLHGSARWADEQDIKAAGLLGNDEGV
YVGAWEDKNGQLQYLRHNGPEHVLTYAPTRSGKGVGLVVPTLLSWKHSTVVADLKGELWAMTAGWRKEYA
KNLCLKFEPAAANGSVAWNPLDEIRVGTENEVGDVQNLATLIVDPDGKGLNDHWQKTSQALLVGVILHVL
YKHKNDGTPATLPYVDSIMADPERGAGELWMEMTQYGHVNGENHPVVGAAGRDMMDRPEEEAGSVLSTAK
SYLALFRDPVVARNVSESHFKIKDLMNHDDPVSLYIVTQPNDKARLRPLVRIMLNMIVRLLADKMEFERV
NNNLTLWQRFKQAFGFSVQGTKRVQTKKTYKHRLLAMIDEFPSLGKLEIIQESLAFLAGYGIKFYLICQD
INQLRSRETGYGPDETITSNCHVQNAYPPNRTETAEHLSKLTGQTTVIKEQITTSGKRASAILGGVSKTM
QEVQRPLLTVDECLRMPGPKKNAEGLITERGDMVVYVAGFPAIYGKQPLFFQDEIFSLRASVPEPKTTDR
IRAAAAANDDASNEAIAI
MRKPNNAVGPQVRNKKKTARGKLIPAMAITSLAAGIQAATQYFAYSFNYQELLGPNFNHIYAPWSYFKWY
SAWHDRLPDAFFAAGSVGATVAAGGLVLTALTSMMKANSSKANEYLHGSARWADEQDIKAAGLLGNDEGV
YVGAWEDKNGQLQYLRHNGPEHVLTYAPTRSGKGVGLVVPTLLSWKHSTVVADLKGELWAMTAGWRKEYA
KNLCLKFEPAAANGSVAWNPLDEIRVGTENEVGDVQNLATLIVDPDGKGLNDHWQKTSQALLVGVILHVL
YKHKNDGTPATLPYVDSIMADPERGAGELWMEMTQYGHVNGENHPVVGAAGRDMMDRPEEEAGSVLSTAK
SYLALFRDPVVARNVSESHFKIKDLMNHDDPVSLYIVTQPNDKARLRPLVRIMLNMIVRLLADKMEFERV
NNNLTLWQRFKQAFGFSVQGTKRVQTKKTYKHRLLAMIDEFPSLGKLEIIQESLAFLAGYGIKFYLICQD
INQLRSRETGYGPDETITSNCHVQNAYPPNRTETAEHLSKLTGQTTVIKEQITTSGKRASAILGGVSKTM
QEVQRPLLTVDECLRMPGPKKNAEGLITERGDMVVYVAGFPAIYGKQPLFFQDEIFSLRASVPEPKTTDR
IRAAAAANDDASNEAIAI
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 16492..26700
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KJE18_RS22965 (KJE18_22920) | 11616..11813 | - | 198 | WP_024252921 | DUF905 family protein | - |
| KJE18_RS22970 (KJE18_22925) | 11903..12487 | - | 585 | WP_270094272 | hypothetical protein | - |
| KJE18_RS22975 (KJE18_22930) | 12484..12732 | - | 249 | WP_059338565 | hypothetical protein | - |
| KJE18_RS22980 (KJE18_22935) | 12732..13040 | - | 309 | WP_003019666 | HigA family addiction module antitoxin | - |
| KJE18_RS22985 (KJE18_22940) | 13049..13348 | - | 300 | WP_024252918 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| KJE18_RS22990 (KJE18_22945) | 14375..15277 | - | 903 | WP_044076637 | plasmid replication initiator TrfA | - |
| KJE18_RS22995 (KJE18_22950) | 15357..15728 | - | 372 | WP_004196205 | single-stranded DNA-binding protein | - |
| KJE18_RS23000 (KJE18_22955) | 15834..16238 | + | 405 | WP_003019654 | transcriptional regulator | - |
| KJE18_RS23005 (KJE18_22960) | 16492..17379 | + | 888 | WP_237083345 | P-type conjugative transfer ATPase TrbB | virB11 |
| KJE18_RS23010 (KJE18_22965) | 17493..17876 | + | 384 | WP_003019650 | TrbC/VirB2 family protein | virB2 |
| KJE18_RS23015 (KJE18_22970) | 17879..18205 | + | 327 | WP_003019648 | conjugal transfer protein TrbD | virB3 |
| KJE18_RS23020 (KJE18_22975) | 18202..20745 | + | 2544 | WP_237083341 | VirB4 family type IV secretion/conjugal transfer ATPase | virb4 |
| KJE18_RS23025 (KJE18_22980) | 20742..21449 | + | 708 | WP_003019644 | VirB8/TrbF family protein | virB8 |
| KJE18_RS23030 (KJE18_22985) | 21465..22320 | + | 856 | Protein_29 | P-type conjugative transfer protein TrbG | - |
| KJE18_RS23035 (KJE18_22990) | 22335..22775 | + | 441 | WP_270094273 | conjugal transfer protein TrbH | - |
| KJE18_RS23040 (KJE18_22995) | 22779..24173 | + | 1395 | WP_003019636 | TrbI/VirB10 family protein | virB10 |
| KJE18_RS23045 (KJE18_23000) | 24186..24956 | + | 771 | WP_003019633 | P-type conjugative transfer protein TrbJ | virB5 |
| KJE18_RS23050 (KJE18_23005) | 24969..25142 | + | 174 | WP_003019629 | entry exclusion lipoprotein TrbK | - |
| KJE18_RS23055 (KJE18_23010) | 25147..26700 | + | 1554 | WP_200020456 | P-type conjugative transfer protein TrbL | virB6 |
| KJE18_RS23060 (KJE18_23015) | 26711..27016 | + | 306 | WP_003019624 | IncN plasmid KikA protein | - |
| KJE18_RS23065 (KJE18_23020) | 27032..27802 | + | 771 | WP_003019622 | conjugal transfer protein TrbN | - |
| KJE18_RS23070 (KJE18_23025) | 27819..28505 | + | 687 | WP_197825536 | TraX family protein | - |
| KJE18_RS23850 | 28949..29044 | + | 96 | WP_003019619 | DinQ-like type I toxin DqlB | - |
| KJE18_RS23075 (KJE18_23030) | 29105..29425 | - | 321 | WP_024252952 | hypothetical protein | - |
| KJE18_RS23080 (KJE18_23035) | 29967..30197 | - | 231 | WP_039696288 | hypothetical protein | - |
| KJE18_RS23085 (KJE18_23040) | 30350..30853 | - | 504 | WP_237083338 | thermonuclease family protein | - |
| KJE18_RS23090 (KJE18_23045) | 30853..31104 | - | 252 | WP_237083337 | hypothetical protein | - |
| KJE18_RS23095 (KJE18_23050) | 31101..31418 | - | 318 | WP_270094274 | hypothetical protein | - |
Host bacterium
| ID | 22154 | GenBank | NZ_CP075346 |
| Plasmid name | pCRE18_1 | Incompatibility group | - |
| Plasmid size | 94151 bp | Coordinate of oriT [Strand] | 51287..51386 [+] |
| Host baterium | Enterobacter kobei strain S18_CRE18 |
Cargo genes
| Drug resistance gene | blaKPC-3, qnrB19, sul1, qacE, dfrA5, aac(6')-Ib-cr, blaOXA-1, catB3, ARR-3 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |