Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   121684
Name   oriT_p51_4_65kb in_silico
Organism   Enterococcus faecium strain Dallas 51_4
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP066403 (18686..18877 [+], 192 nt)
oriT length   192 nt
IRs (inverted repeats)      106..112, 122..128  (ATTTTTT..AAAAAAT)
 107..113, 120..126  (TTTTTTG..CAAAAAA)
 93..98, 105..110  (AAAATA..TATTTT)
 65..71, 75..81  (AGTTGGC..GCCAACT)
 41..49, 52..60  (CACCTTCCT..AGGAAGGTG)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 192 nt

>oriT_p51_4_65kb
CCAAGGAATTAATACAAAGAGCATAAGGGAAAACTGATAGCACCTTCCTAAAGGAAGGTGGCTAAGTTGGCTGTGCCAACTGGTTTTCTTTCAAAATAGCTTCATATTTTTTGCTATCACAAAAAAATCCATTTTCGACCTATTTTCGGTCATAATATAGTACCTACTTTTGGTCATAGTTTCGTCCGTAGT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   13681 GenBank   WP_002326870
Name   mobP2_JCQ06_RS15465_p51_4_65kb insolico UniProt ID   B5U8V6
Length   506 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 506 a.a.        Molecular weight: 60597.01 Da        Isoelectric Point: 9.3485

>WP_002326870.1 MULTISPECIES: MobP2 family relaxase [Enterococcus]
MTEHIFNRKSASIILKAKFETGKSKKNNHMVELQKFVDYISRQEAIRQDKLDHDYSEDELNELARIEKAL
EKIEQETDQPVIKDLREMDKYIDYMTRKKAILENVDNEIVNGAFSNTKRYITKKDISKIKESVIEAKNNG
SVMFQDVISFDNDFLVREGYYNPETNELNENVLYEATKSMMGKMQEKEELVDPFWFATIHRNTEHIHIHV
TAMERKNTREIMEYDGVLQARGKRKQSTLDDMIFKFGSKILDRTNEFEKISKLRKEVPLELKQSVKDSLI
QLYVENNSRYDKELVKYLKELKKEIPSTTRGYNELPEETKEKIDEVTNYMTKDNPKRKEYDRMTKEIDEL
YQTTYGKRYNPQEYYLNRKKDFNARMGNAVVGQIKLLKQSEEKNLKSNELKNLLDGKKLNVKFDREKPEF
KTKYAKQSYDDFQKRLEERQLAREKNEVHFKKISEKFKDKKLIRKIDRVINDDLNMYRAKNDYEVAQRMI
EQEKMRQAREFEIGMN

  Protein domains


Predicted by InterproScan.

MobL relaxases (MobL)

(11-438)


  Protein structure


Source ID Structure
AlphaFold DB B5U8V6


T4CP

ID   15990 GenBank   WP_002311855
Name   t4cp2_JCQ06_RS15535_p51_4_65kb insolico UniProt ID   _
Length   952 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 952 a.a.        Molecular weight: 108225.12 Da        Isoelectric Point: 7.4751

>WP_002311855.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Enterococcus]
MKLVQNNEVKEIKNGFVKNSNKILGKLKKVGSFKKNKYHTGKIEGEDLPKSLQDKLNVPLVFIGVFLGSI
VFLAITYLLNFLKALLVAIGQSESGLLGVEFDPKFSLAYLKPLGDTKQYFMCAVVAVIGVAYLIYGKLNH
ESEKNLVYGQKGDSRLSTIKEIKRDYHEIPEKTEVYDGIGGVPISHYQDKYYIDRDTVNTVVLGASRSGK
GETFVVPLLDNLSRAKIQSNMVVNDPKGELFSASKETLEKRGYRVLVLNIDDPLESMSFNPLQLVIDSWA
NKDYHEASKRANTLTSMLFASGMGTDNEFFYKSAKSAVNAIILTIVEHCFNNDCIEKITMYNVAQMLNEL
GSLFYTDPKTGKEKNALDEYFNTLPQGNQAKIQYGSTSFAGDKAKGSILSTASQGIEMFTSDLFGKLTSK
MSIDLKEIGFPKSIQFKVNTNLVGKRISISFLRKENDVIRLIKKYRVKVKALGLCVLNFNEYLQDGDMLD
IRYEEEKSRAWYRIEFPKKQANSHDTSLVTKKFKSGKNYSDLEISTLRLKYTDQPTAVFMVIPDYDPSNH
VLASIFISQLYTELASNCKQTPDKKCFRRVHFLLDEFGNMPAIDNMDGIMTVCLGRNMLFDLVIQSYSQL
ETRYDKAFKTIKENCQNHILIMSNDEETIEEISKKCGHKTTINRSASSKHLDTDSSVTSSAEQERVITVE
RASQLIEGEQIILRNLHRQDTKRNKIRPFPIFNTKETNMPYRYQFLAEDFDTQRDINEIDIACEHINLSL
QDNQIPYARFIKDLKTRLEYSIINNIPISEEDYQDYLNLSGSQQTINEEELLKLVNTEVKSQDMEGQVLS
EQEMKINIYLKESQKEAIELVKVINGRLADENSADMVIKTINSQILDTPFIQSLINDSIAIHNVQESKDQ
IMILEQQLANAYELAVFKTKNSILKNKIIKLKNAMTKLAKAI

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(199-442)

TraM recognition site of TraD and TraG (TraG-D_C)

(589-701)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 10206..40450

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JCQ06_RS15390 (JCQ06_15390) 5265..5642 + 378 WP_002305129 type II toxin-antitoxin system HicB family antitoxin -
JCQ06_RS15395 (JCQ06_15395) 6202..7374 - 1173 WP_002311882 hypothetical protein -
JCQ06_RS15400 (JCQ06_15400) 7618..8586 - 969 WP_002311881 zeta toxin family protein -
JCQ06_RS15405 (JCQ06_15405) 8583..8780 - 198 WP_002311880 hypothetical protein -
JCQ06_RS15410 (JCQ06_15410) 8791..9108 - 318 WP_002311879 hypothetical protein -
JCQ06_RS15415 (JCQ06_15415) 9145..9363 - 219 WP_002311878 hypothetical protein -
JCQ06_RS15420 (JCQ06_15420) 9472..9663 - 192 WP_002311877 hypothetical protein -
JCQ06_RS15425 (JCQ06_15425) 9825..10181 - 357 WP_002311876 hypothetical protein -
JCQ06_RS15430 (JCQ06_15430) 10206..11210 - 1005 WP_002311875 DUF3991 domain-containing protein traP
JCQ06_RS15435 (JCQ06_15435) 11247..11507 - 261 WP_002311874 hypothetical protein -
JCQ06_RS15440 (JCQ06_15440) 11516..12589 - 1074 WP_002311873 hypothetical protein -
JCQ06_RS15445 (JCQ06_15445) 12597..14423 - 1827 WP_002311872 ArdC-like ssDNA-binding domain-containing protein -
JCQ06_RS15450 (JCQ06_15450) 14578..15444 - 867 WP_002311871 hypothetical protein -
JCQ06_RS15455 (JCQ06_15455) 15619..15882 - 264 WP_002311870 hypothetical protein -
JCQ06_RS15460 (JCQ06_15460) 15884..16213 - 330 WP_002311869 hypothetical protein -
JCQ06_RS15465 (JCQ06_15465) 16235..17755 - 1521 WP_002326870 MobP2 family relaxase -
JCQ06_RS15470 (JCQ06_15470) 17837..18304 - 468 WP_002311867 hypothetical protein -
JCQ06_RS15475 (JCQ06_15475) 18301..18597 - 297 WP_002311865 hypothetical protein -
JCQ06_RS15480 (JCQ06_15480) 18948..19178 - 231 WP_002311864 hypothetical protein -
JCQ06_RS15485 (JCQ06_15485) 19214..20110 - 897 WP_002311863 hypothetical protein -
JCQ06_RS15490 (JCQ06_15490) 20124..20774 - 651 WP_002311862 cell division protein FtsL -
JCQ06_RS15495 (JCQ06_15495) 20789..21925 - 1137 WP_002311861 bifunctional lytic transglycosylase/C40 family peptidase orf14
JCQ06_RS15500 (JCQ06_15500) 21947..22267 - 321 WP_010729568 hypothetical protein virb4
JCQ06_RS15505 (JCQ06_15505) 22455..24332 - 1878 WP_002305177 group II intron reverse transcriptase/maturase -
JCQ06_RS15510 (JCQ06_15510) 25020..26645 - 1626 WP_010729563 VirB4 family type IV secretion system protein virb4
JCQ06_RS15515 (JCQ06_15515) 26661..27128 - 468 WP_002311859 hypothetical protein -
JCQ06_RS15520 (JCQ06_15520) 27144..27761 - 618 WP_002311858 hypothetical protein virb4
JCQ06_RS15525 (JCQ06_15525) 27758..28069 - 312 WP_002317413 DUF5592 family protein -
JCQ06_RS15530 (JCQ06_15530) 28117..30786 - 2670 WP_002326868 pLS20_p028 family conjugation system transmembrane protein -
JCQ06_RS15535 (JCQ06_15535) 30804..33662 - 2859 WP_002311855 VirD4-like conjugal transfer protein, CD1115 family -
JCQ06_RS15540 (JCQ06_15540) 33675..34391 - 717 WP_002311854 hypothetical protein -
JCQ06_RS15545 (JCQ06_15545) 34404..36230 - 1827 WP_002311853 hypothetical protein -
JCQ06_RS15550 (JCQ06_15550) 36339..36614 - 276 WP_002311852 TrbC/VirB2 family protein virB2
JCQ06_RS15555 (JCQ06_15555) 36633..36854 - 222 WP_002326867 hypothetical protein -
JCQ06_RS15560 (JCQ06_15560) 36866..37012 - 147 WP_002326866 hypothetical protein -
JCQ06_RS15565 (JCQ06_15565) 37026..37388 - 363 WP_002311851 DUF4320 family protein -
JCQ06_RS15570 (JCQ06_15570) 37406..38203 - 798 WP_002311850 hypothetical protein -
JCQ06_RS15575 (JCQ06_15575) 38203..39018 - 816 WP_002313315 hypothetical protein -
JCQ06_RS15580 (JCQ06_15580) 39035..40450 - 1416 WP_002311848 CpaF/VirB11 family protein virB11
JCQ06_RS15585 (JCQ06_15585) 40462..40884 - 423 WP_002311847 hypothetical protein -
JCQ06_RS15590 (JCQ06_15590) 40952..41104 - 153 WP_002311846 hypothetical protein -
JCQ06_RS15595 (JCQ06_15595) 41118..41480 - 363 WP_002311845 hypothetical protein -
JCQ06_RS15600 (JCQ06_15600) 41540..42325 - 786 WP_046669026 hypothetical protein -
JCQ06_RS15605 (JCQ06_15605) 42475..43701 + 1227 WP_046650365 IS21 family transposase -
JCQ06_RS15610 (JCQ06_15610) 43716..44471 + 756 WP_010818485 IS21-like element helper ATPase IstB -

Host bacterium

ID   22111 GenBank   NZ_CP066403
Plasmid name   p51_4_65kb Incompatibility group   -
Plasmid size   65409 bp Coordinate of oriT [Strand]   18686..18877 [+]
Host baterium   Enterococcus faecium strain Dallas 51_4

Cargo genes

Drug resistance gene   dfrG
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA1