Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 121682 |
| Name | oriT_p144_1_66kb  |
| Organism | Enterococcus faecium strain Dallas 144_1 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP066639 (36534..36725 [-], 192 nt) |
| oriT length | 192 nt |
| IRs (inverted repeats) | 106..112, 122..128 (ATTTTTT..AAAAAAT) 107..113, 120..126 (TTTTTTG..CAAAAAA) 93..98, 105..110 (AAAATA..TATTTT) 65..71, 75..81 (AGTTGGC..GCCAACT) 41..49, 52..60 (CACCTTCCT..AGGAAGGTG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 192 nt
>oriT_p144_1_66kb
CCAAGGAATTAATACAAAGAGCATAAGGGAAAACTGATAGCACCTTCCTAAAGGAAGGTGGCTAAGTTGGCTGTGCCAACTGGTTTTCTTTCAAAATAGCTTCATATTTTTTGCTATCACAAAAAAATCCATTTTCGACCTATTTTCGGTCATAATATAGTACCTACTTTTGGTCATAGTTTCGTCCGTAGT
CCAAGGAATTAATACAAAGAGCATAAGGGAAAACTGATAGCACCTTCCTAAAGGAAGGTGGCTAAGTTGGCTGTGCCAACTGGTTTTCTTTCAAAATAGCTTCATATTTTTTGCTATCACAAAAAAATCCATTTTCGACCTATTTTCGGTCATAATATAGTACCTACTTTTGGTCATAGTTTCGTCCGTAGT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 13680 | GenBank | WP_002326870 |
| Name | mobP2_JCP95_RS15110_p144_1_66kb |
UniProt ID | B5U8V6 |
| Length | 506 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 506 a.a. Molecular weight: 60597.01 Da Isoelectric Point: 9.3485
>WP_002326870.1 MULTISPECIES: MobP2 family relaxase [Enterococcus]
MTEHIFNRKSASIILKAKFETGKSKKNNHMVELQKFVDYISRQEAIRQDKLDHDYSEDELNELARIEKAL
EKIEQETDQPVIKDLREMDKYIDYMTRKKAILENVDNEIVNGAFSNTKRYITKKDISKIKESVIEAKNNG
SVMFQDVISFDNDFLVREGYYNPETNELNENVLYEATKSMMGKMQEKEELVDPFWFATIHRNTEHIHIHV
TAMERKNTREIMEYDGVLQARGKRKQSTLDDMIFKFGSKILDRTNEFEKISKLRKEVPLELKQSVKDSLI
QLYVENNSRYDKELVKYLKELKKEIPSTTRGYNELPEETKEKIDEVTNYMTKDNPKRKEYDRMTKEIDEL
YQTTYGKRYNPQEYYLNRKKDFNARMGNAVVGQIKLLKQSEEKNLKSNELKNLLDGKKLNVKFDREKPEF
KTKYAKQSYDDFQKRLEERQLAREKNEVHFKKISEKFKDKKLIRKIDRVINDDLNMYRAKNDYEVAQRMI
EQEKMRQAREFEIGMN
MTEHIFNRKSASIILKAKFETGKSKKNNHMVELQKFVDYISRQEAIRQDKLDHDYSEDELNELARIEKAL
EKIEQETDQPVIKDLREMDKYIDYMTRKKAILENVDNEIVNGAFSNTKRYITKKDISKIKESVIEAKNNG
SVMFQDVISFDNDFLVREGYYNPETNELNENVLYEATKSMMGKMQEKEELVDPFWFATIHRNTEHIHIHV
TAMERKNTREIMEYDGVLQARGKRKQSTLDDMIFKFGSKILDRTNEFEKISKLRKEVPLELKQSVKDSLI
QLYVENNSRYDKELVKYLKELKKEIPSTTRGYNELPEETKEKIDEVTNYMTKDNPKRKEYDRMTKEIDEL
YQTTYGKRYNPQEYYLNRKKDFNARMGNAVVGQIKLLKQSEEKNLKSNELKNLLDGKKLNVKFDREKPEF
KTKYAKQSYDDFQKRLEERQLAREKNEVHFKKISEKFKDKKLIRKIDRVINDDLNMYRAKNDYEVAQRMI
EQEKMRQAREFEIGMN
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | B5U8V6 |
T4CP
| ID | 15989 | GenBank | WP_002311855 |
| Name | t4cp2_JCP95_RS15040_p144_1_66kb |
UniProt ID | _ |
| Length | 952 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 952 a.a. Molecular weight: 108225.12 Da Isoelectric Point: 7.4751
>WP_002311855.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Enterococcus]
MKLVQNNEVKEIKNGFVKNSNKILGKLKKVGSFKKNKYHTGKIEGEDLPKSLQDKLNVPLVFIGVFLGSI
VFLAITYLLNFLKALLVAIGQSESGLLGVEFDPKFSLAYLKPLGDTKQYFMCAVVAVIGVAYLIYGKLNH
ESEKNLVYGQKGDSRLSTIKEIKRDYHEIPEKTEVYDGIGGVPISHYQDKYYIDRDTVNTVVLGASRSGK
GETFVVPLLDNLSRAKIQSNMVVNDPKGELFSASKETLEKRGYRVLVLNIDDPLESMSFNPLQLVIDSWA
NKDYHEASKRANTLTSMLFASGMGTDNEFFYKSAKSAVNAIILTIVEHCFNNDCIEKITMYNVAQMLNEL
GSLFYTDPKTGKEKNALDEYFNTLPQGNQAKIQYGSTSFAGDKAKGSILSTASQGIEMFTSDLFGKLTSK
MSIDLKEIGFPKSIQFKVNTNLVGKRISISFLRKENDVIRLIKKYRVKVKALGLCVLNFNEYLQDGDMLD
IRYEEEKSRAWYRIEFPKKQANSHDTSLVTKKFKSGKNYSDLEISTLRLKYTDQPTAVFMVIPDYDPSNH
VLASIFISQLYTELASNCKQTPDKKCFRRVHFLLDEFGNMPAIDNMDGIMTVCLGRNMLFDLVIQSYSQL
ETRYDKAFKTIKENCQNHILIMSNDEETIEEISKKCGHKTTINRSASSKHLDTDSSVTSSAEQERVITVE
RASQLIEGEQIILRNLHRQDTKRNKIRPFPIFNTKETNMPYRYQFLAEDFDTQRDINEIDIACEHINLSL
QDNQIPYARFIKDLKTRLEYSIINNIPISEEDYQDYLNLSGSQQTINEEELLKLVNTEVKSQDMEGQVLS
EQEMKINIYLKESQKEAIELVKVINGRLADENSADMVIKTINSQILDTPFIQSLINDSIAIHNVQESKDQ
IMILEQQLANAYELAVFKTKNSILKNKIIKLKNAMTKLAKAI
MKLVQNNEVKEIKNGFVKNSNKILGKLKKVGSFKKNKYHTGKIEGEDLPKSLQDKLNVPLVFIGVFLGSI
VFLAITYLLNFLKALLVAIGQSESGLLGVEFDPKFSLAYLKPLGDTKQYFMCAVVAVIGVAYLIYGKLNH
ESEKNLVYGQKGDSRLSTIKEIKRDYHEIPEKTEVYDGIGGVPISHYQDKYYIDRDTVNTVVLGASRSGK
GETFVVPLLDNLSRAKIQSNMVVNDPKGELFSASKETLEKRGYRVLVLNIDDPLESMSFNPLQLVIDSWA
NKDYHEASKRANTLTSMLFASGMGTDNEFFYKSAKSAVNAIILTIVEHCFNNDCIEKITMYNVAQMLNEL
GSLFYTDPKTGKEKNALDEYFNTLPQGNQAKIQYGSTSFAGDKAKGSILSTASQGIEMFTSDLFGKLTSK
MSIDLKEIGFPKSIQFKVNTNLVGKRISISFLRKENDVIRLIKKYRVKVKALGLCVLNFNEYLQDGDMLD
IRYEEEKSRAWYRIEFPKKQANSHDTSLVTKKFKSGKNYSDLEISTLRLKYTDQPTAVFMVIPDYDPSNH
VLASIFISQLYTELASNCKQTPDKKCFRRVHFLLDEFGNMPAIDNMDGIMTVCLGRNMLFDLVIQSYSQL
ETRYDKAFKTIKENCQNHILIMSNDEETIEEISKKCGHKTTINRSASSKHLDTDSSVTSSAEQERVITVE
RASQLIEGEQIILRNLHRQDTKRNKIRPFPIFNTKETNMPYRYQFLAEDFDTQRDINEIDIACEHINLSL
QDNQIPYARFIKDLKTRLEYSIINNIPISEEDYQDYLNLSGSQQTINEEELLKLVNTEVKSQDMEGQVLS
EQEMKINIYLKESQKEAIELVKVINGRLADENSADMVIKTINSQILDTPFIQSLINDSIAIHNVQESKDQ
IMILEQQLANAYELAVFKTKNSILKNKIIKLKNAMTKLAKAI
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 14961..34622
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| JCP95_RS14970 (JCP95_14970) | 10940..11695 | - | 756 | WP_010818485 | IS21-like element helper ATPase IstB | - |
| JCP95_RS14975 (JCP95_14975) | 11710..12936 | - | 1227 | WP_046650365 | IS21 family transposase | - |
| JCP95_RS14980 (JCP95_14980) | 13086..13871 | + | 786 | WP_046669026 | hypothetical protein | - |
| JCP95_RS14985 (JCP95_14985) | 13931..14293 | + | 363 | WP_002311845 | hypothetical protein | - |
| JCP95_RS14990 (JCP95_14990) | 14307..14459 | + | 153 | WP_002311846 | hypothetical protein | - |
| JCP95_RS14995 (JCP95_14995) | 14527..14949 | + | 423 | WP_002311847 | hypothetical protein | - |
| JCP95_RS15000 (JCP95_15000) | 14961..16376 | + | 1416 | WP_002311848 | CpaF/VirB11 family protein | virB11 |
| JCP95_RS15005 (JCP95_15005) | 16393..17208 | + | 816 | WP_002313315 | hypothetical protein | - |
| JCP95_RS15010 (JCP95_15010) | 17208..18005 | + | 798 | WP_002311850 | hypothetical protein | - |
| JCP95_RS15015 (JCP95_15015) | 18023..18385 | + | 363 | WP_002311851 | DUF4320 family protein | - |
| JCP95_RS15020 (JCP95_15020) | 18557..18778 | + | 222 | WP_002326867 | hypothetical protein | - |
| JCP95_RS15025 (JCP95_15025) | 18797..19072 | + | 276 | WP_002311852 | TrbC/VirB2 family protein | virB2 |
| JCP95_RS15030 (JCP95_15030) | 19181..21007 | + | 1827 | WP_002311853 | hypothetical protein | - |
| JCP95_RS15035 (JCP95_15035) | 21020..21736 | + | 717 | WP_002311854 | hypothetical protein | - |
| JCP95_RS15040 (JCP95_15040) | 21749..24607 | + | 2859 | WP_002311855 | VirD4-like conjugal transfer protein, CD1115 family | - |
| JCP95_RS15045 (JCP95_15045) | 24625..27294 | + | 2670 | WP_002326868 | pLS20_p028 family conjugation system transmembrane protein | - |
| JCP95_RS15050 (JCP95_15050) | 27342..27653 | + | 312 | WP_002317413 | DUF5592 family protein | - |
| JCP95_RS15055 (JCP95_15055) | 27650..28267 | + | 618 | WP_002311858 | hypothetical protein | virb4 |
| JCP95_RS15060 (JCP95_15060) | 28283..28750 | + | 468 | WP_002311859 | hypothetical protein | - |
| JCP95_RS15065 (JCP95_15065) | 28766..30391 | + | 1626 | WP_010729563 | VirB4 family type IV secretion system protein | virb4 |
| JCP95_RS15070 (JCP95_15070) | 31079..32956 | + | 1878 | WP_002305177 | group II intron reverse transcriptase/maturase | - |
| JCP95_RS15075 (JCP95_15075) | 33153..33464 | + | 312 | WP_025477190 | hypothetical protein | virb4 |
| JCP95_RS15080 (JCP95_15080) | 33486..34622 | + | 1137 | WP_002311861 | bifunctional lytic transglycosylase/C40 family peptidase | orf14 |
| JCP95_RS15085 (JCP95_15085) | 34637..35287 | + | 651 | WP_002311862 | cell division protein FtsL | - |
| JCP95_RS15090 (JCP95_15090) | 35301..36197 | + | 897 | WP_002311863 | hypothetical protein | - |
| JCP95_RS15095 (JCP95_15095) | 36233..36463 | + | 231 | WP_002311864 | hypothetical protein | - |
| JCP95_RS15100 (JCP95_15100) | 36814..37110 | + | 297 | WP_002311865 | hypothetical protein | - |
| JCP95_RS15105 (JCP95_15105) | 37107..37574 | + | 468 | WP_002311867 | hypothetical protein | - |
| JCP95_RS15110 (JCP95_15110) | 37656..39176 | + | 1521 | WP_002326870 | MobP2 family relaxase | - |
| JCP95_RS15115 (JCP95_15115) | 39198..39527 | + | 330 | WP_002311869 | hypothetical protein | - |
Host bacterium
| ID | 22109 | GenBank | NZ_CP066639 |
| Plasmid name | p144_1_66kb | Incompatibility group | - |
| Plasmid size | 66742 bp | Coordinate of oriT [Strand] | 36534..36725 [-] |
| Host baterium | Enterococcus faecium strain Dallas 144_1 |
Cargo genes
| Drug resistance gene | dfrG |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA1 |