Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   121680
Name   oriT_pSa1753 in_silico
Organism   Salmonella sp. strain Sa1735
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MT859309 (144603..144753 [-], 151 nt)
oriT length   151 nt
IRs (inverted repeats)      93..98, 106..111  (TGGCCT..AGGCCA)
 12..17, 24..29  (AACCCT..AGGGTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 151 nt

>oriT_pSa1753
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   13678 GenBank   WP_064986968
Name   mobH_JG757_RS00885_pSa1753 insolico UniProt ID   A0A6D1JL47
Length   990 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 990 a.a.        Molecular weight: 109328.85 Da        Isoelectric Point: 4.9395

>WP_064986968.1 MULTISPECIES: MobH family relaxase [Enterobacterales]
MLKALNKLFGGRSGVIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGSTESSKPD
AGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDA
FSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQNSLQGDDFPMFGSSDEPPSWAIEPLPMLTDA
PEQTTPAPAMPPTDKPNLHEKDAKTLLVEMLAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILY
PDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDA
FELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSGVT
PQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQ
GKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure


Source ID Structure
AlphaFold DB A0A6D1JL47


T4CP

ID   15986 GenBank   WP_000637384
Name   traC_JG757_RS00775_pSa1753 insolico UniProt ID   _
Length   815 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 815 a.a.        Molecular weight: 92604.86 Da        Isoelectric Point: 5.9216

>WP_000637384.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIVTIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSAGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTTLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTDRIFAKTNKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQQLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSQGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRRQAA

  Protein domains


Predicted by InterproScan.

Family of unknown function (DUF5934) (DUF5934)

(284-434)

TraC protein (TraC_F_IV)

(21-255)

TraG P-loop domain (P-loop_TraG)

(449-809)

  Protein structure



No available structure.



ID   15987 GenBank   WP_000178857
Name   traD_JG757_RS00880_pSa1753 insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69225.71 Da        Isoelectric Point: 6.9403

>WP_000178857.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPDDMWLGSGFLWENRHAQRVFEILKRDWTSIVGRESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVMPDWEAEAAAYLEKVKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-299)

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 137579..146669

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG757_RS00790 (EGLDKNOD_00160) 132604..132969 - 366 Protein_159 LuxR family transcriptional regulator -
JG757_RS00795 (EGLDKNOD_00161) 133226..133543 + 318 WP_000118520 quaternary ammonium compound efflux SMR transporter SugE -
JG757_RS00800 (EGLDKNOD_00162) 133540..134073 - 534 WP_001221666 lipocalin family protein -
JG757_RS00805 (EGLDKNOD_00163) 134167..135312 - 1146 WP_000976514 extended-spectrum class C beta-lactamase CMY-2 -
JG757_RS00810 (EGLDKNOD_00165) 135636..136898 - 1263 WP_000608644 IS1380-like element ISEcp1 family transposase -
JG757_RS00815 (EGLDKNOD_00166) 137183..137575 - 393 WP_000479535 TraA family conjugative transfer protein -
JG757_RS00820 (EGLDKNOD_00167) 137579..138157 - 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
JG757_RS00825 (EGLDKNOD_00168) 138154..139467 - 1314 WP_024131605 TraB/VirB10 family protein traB
JG757_RS00830 (EGLDKNOD_00169) 139467..140384 - 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
JG757_RS00835 (EGLDKNOD_00170) 140368..140994 - 627 WP_001049717 TraE/TraK family type IV conjugative transfer system protein traE
JG757_RS00840 (EGLDKNOD_00171) 140991..141272 - 282 WP_000805625 type IV conjugative transfer system protein TraL traL
JG757_RS00845 (EGLDKNOD_00172) 141417..141794 - 378 WP_001326174 hypothetical protein -
JG757_RS00850 (EGLDKNOD_00173) 141794..141937 - 144 WP_001275801 hypothetical protein -
JG757_RS00855 (EGLDKNOD_00174) 142118..142780 - 663 WP_001231464 hypothetical protein -
JG757_RS00860 (EGLDKNOD_00175) 142780..143157 - 378 WP_000869297 hypothetical protein -
JG757_RS00865 (EGLDKNOD_00176) 143167..143613 - 447 WP_000122507 hypothetical protein -
JG757_RS00870 (EGLDKNOD_00177) 143623..144252 - 630 WP_000743449 DUF4400 domain-containing protein tfc7
JG757_RS00875 (EGLDKNOD_00178) 144209..144793 - 585 WP_000332868 hypothetical protein -
JG757_RS00880 (EGLDKNOD_00179) 144804..146669 - 1866 WP_000178857 conjugative transfer system coupling protein TraD virb4
JG757_RS00885 (EGLDKNOD_00180) 146666..149638 - 2973 WP_064986968 MobH family relaxase -
JG757_RS00890 (EGLDKNOD_00181) 149806..150423 + 618 WP_001249396 hypothetical protein -
JG757_RS00895 (EGLDKNOD_00182) 150405..150638 + 234 WP_001191892 hypothetical protein -

Host bacterium

ID   22107 GenBank   NZ_MT859309
Plasmid name   pSa1753 Incompatibility group   IncA/C2
Plasmid size   169152 bp Coordinate of oriT [Strand]   144603..144753 [-]
Host baterium   Salmonella sp. strain Sa1735

Cargo genes

Drug resistance gene   floR, mph(A), dfrA14, ant(3'')-Ia, blaOXA-10, aac(6')-Ib-cr, qnrVC1, blaCMY-2, sul2, aph(3'')-Ib, aph(6)-Id, tet(A)
Virulence gene   -
Metal resistance gene   merR, merT, merP, merA, merB, merD, merE
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -