Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 121668
• Name: oriT_pBNS11-29kb
• Organism: Ligilactobacillus salivarius strain BNS11
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP089853 (16714..16764 [+], 51 nt)
• oriT length: 51 nt
• IRs (inverted repeats): 13..18, 25..30 (TCCCAC..GTGGGA)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 51 nt

>oriT_pBNS11-29kb
GATACTATCGGCTCCCACGCACCTGTGGGACCATTTTCTCTTATGCTCTTT

oriT secondary structure

Predicted by RNAfold.

Relaxase

Rep_trans_LVD18_RS11350_pBNS11-29kb

• ID: 13669
• GenBank: WP_232751573
• Name: Rep_trans_LVD18_RS11350_pBNS11-29kb
• UniProt ID: _
• Length: 361 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 361 a.a.
• Molecular weight: 42571.98 Da
• Isoelectric Point: 9.8118

>WP_232751573.1 replication initiation factor domain-containing protein [Ligilactobacillus salivarius]
MYTFYYIREKEMPVTSTIKQTLQTSIDWLEFTIFQLSYEDVIERILQLKMEDFSDLSKGKFGYNAQTKWG
NGNLFILFNQVDDEPCINHMGVHVILSGTGCRAYESQKSLYTLINVLVALENQAKLSRIDLAIDDFKDEL
IKFSRIRKAALRSEFTSRWNKWDELNSRSTGDGKLLGQTMYFGSQTSDIFCRIYDKALEQKRKAKEKDKE
IPEKWTRLEIVYRKERAKMLALHMIECSNVGLVIRRTLQQYIRFVQRPKNSKDSNKSRWKTAKWWERLLE
NVEPLKLTIKKQDKTVDEMIKWLDKQIGPTLATVVTALDGEINWLYKLISNGNTRLKNKHQQAIFQYKQE
KEKERKGDENG

Protein domains

Predicted by InterproScan.

• Replication initiation factor (Rep_trans) (125-319)

• Rolling Circle replication initiation protein N-terminal domain (Rol_Rep_N) (24-106)

Protein structure

No available structure.

Host bacterium

• ID: 22095
• GenBank: NZ_CP089853
• Plasmid name: pBNS11-29kb
• Incompatibility group: -
• Plasmid size: 28852 bp
• Coordinate of oriT [Strand]: 16714..16764 [+]
• Host baterium: Ligilactobacillus salivarius strain BNS11

Cargo genes

• Drug resistance gene: tet(L), tet(M)
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -