Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 121667 |
| Name | oriT_p66_65kb  |
| Organism | Enterococcus faecium strain Dallas 66 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP066436 (64937..65128 [+], 192 nt) |
| oriT length | 192 nt |
| IRs (inverted repeats) | 106..112, 122..128 (ATTTTTT..AAAAAAT) 107..113, 120..126 (TTTTTTG..CAAAAAA) 93..98, 105..110 (AAAATA..TATTTT) 65..71, 75..81 (AGTTGGC..GCCAACT) 41..49, 52..60 (CACCTTCCT..AGGAAGGTG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 192 nt
>oriT_p66_65kb
CCAAGGAATTAATACAAAGAGCATAAGGGAAAACTGATAGCACCTTCCTAAAGGAAGGTGGCTAAGTTGGCTGTGCCAACTGGTTTTCTTTCAAAATAGCTTCATATTTTTTGCTATCACAAAAAAATCCATTTTCGACCTATTTTCGGTCATAATATAGTACCTACTTTTGGTCATAGTTTCGTCCGTAGT
CCAAGGAATTAATACAAAGAGCATAAGGGAAAACTGATAGCACCTTCCTAAAGGAAGGTGGCTAAGTTGGCTGTGCCAACTGGTTTTCTTTCAAAATAGCTTCATATTTTTTGCTATCACAAAAAAATCCATTTTCGACCTATTTTCGGTCATAATATAGTACCTACTTTTGGTCATAGTTTCGTCCGTAGT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 13668 | GenBank | WP_002326870 |
| Name | mobP2_JCP89_RS15220_p66_65kb |
UniProt ID | B5U8V6 |
| Length | 506 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 506 a.a. Molecular weight: 60597.01 Da Isoelectric Point: 9.3485
>WP_002326870.1 MULTISPECIES: MobP2 family relaxase [Enterococcus]
MTEHIFNRKSASIILKAKFETGKSKKNNHMVELQKFVDYISRQEAIRQDKLDHDYSEDELNELARIEKAL
EKIEQETDQPVIKDLREMDKYIDYMTRKKAILENVDNEIVNGAFSNTKRYITKKDISKIKESVIEAKNNG
SVMFQDVISFDNDFLVREGYYNPETNELNENVLYEATKSMMGKMQEKEELVDPFWFATIHRNTEHIHIHV
TAMERKNTREIMEYDGVLQARGKRKQSTLDDMIFKFGSKILDRTNEFEKISKLRKEVPLELKQSVKDSLI
QLYVENNSRYDKELVKYLKELKKEIPSTTRGYNELPEETKEKIDEVTNYMTKDNPKRKEYDRMTKEIDEL
YQTTYGKRYNPQEYYLNRKKDFNARMGNAVVGQIKLLKQSEEKNLKSNELKNLLDGKKLNVKFDREKPEF
KTKYAKQSYDDFQKRLEERQLAREKNEVHFKKISEKFKDKKLIRKIDRVINDDLNMYRAKNDYEVAQRMI
EQEKMRQAREFEIGMN
MTEHIFNRKSASIILKAKFETGKSKKNNHMVELQKFVDYISRQEAIRQDKLDHDYSEDELNELARIEKAL
EKIEQETDQPVIKDLREMDKYIDYMTRKKAILENVDNEIVNGAFSNTKRYITKKDISKIKESVIEAKNNG
SVMFQDVISFDNDFLVREGYYNPETNELNENVLYEATKSMMGKMQEKEELVDPFWFATIHRNTEHIHIHV
TAMERKNTREIMEYDGVLQARGKRKQSTLDDMIFKFGSKILDRTNEFEKISKLRKEVPLELKQSVKDSLI
QLYVENNSRYDKELVKYLKELKKEIPSTTRGYNELPEETKEKIDEVTNYMTKDNPKRKEYDRMTKEIDEL
YQTTYGKRYNPQEYYLNRKKDFNARMGNAVVGQIKLLKQSEEKNLKSNELKNLLDGKKLNVKFDREKPEF
KTKYAKQSYDDFQKRLEERQLAREKNEVHFKKISEKFKDKKLIRKIDRVINDDLNMYRAKNDYEVAQRMI
EQEKMRQAREFEIGMN
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | B5U8V6 |
T4CP
| ID | 15973 | GenBank | WP_002311855 |
| Name | t4cp2_JCP89_RS14930_p66_65kb |
UniProt ID | _ |
| Length | 952 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 952 a.a. Molecular weight: 108225.12 Da Isoelectric Point: 7.4751
>WP_002311855.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Enterococcus]
MKLVQNNEVKEIKNGFVKNSNKILGKLKKVGSFKKNKYHTGKIEGEDLPKSLQDKLNVPLVFIGVFLGSI
VFLAITYLLNFLKALLVAIGQSESGLLGVEFDPKFSLAYLKPLGDTKQYFMCAVVAVIGVAYLIYGKLNH
ESEKNLVYGQKGDSRLSTIKEIKRDYHEIPEKTEVYDGIGGVPISHYQDKYYIDRDTVNTVVLGASRSGK
GETFVVPLLDNLSRAKIQSNMVVNDPKGELFSASKETLEKRGYRVLVLNIDDPLESMSFNPLQLVIDSWA
NKDYHEASKRANTLTSMLFASGMGTDNEFFYKSAKSAVNAIILTIVEHCFNNDCIEKITMYNVAQMLNEL
GSLFYTDPKTGKEKNALDEYFNTLPQGNQAKIQYGSTSFAGDKAKGSILSTASQGIEMFTSDLFGKLTSK
MSIDLKEIGFPKSIQFKVNTNLVGKRISISFLRKENDVIRLIKKYRVKVKALGLCVLNFNEYLQDGDMLD
IRYEEEKSRAWYRIEFPKKQANSHDTSLVTKKFKSGKNYSDLEISTLRLKYTDQPTAVFMVIPDYDPSNH
VLASIFISQLYTELASNCKQTPDKKCFRRVHFLLDEFGNMPAIDNMDGIMTVCLGRNMLFDLVIQSYSQL
ETRYDKAFKTIKENCQNHILIMSNDEETIEEISKKCGHKTTINRSASSKHLDTDSSVTSSAEQERVITVE
RASQLIEGEQIILRNLHRQDTKRNKIRPFPIFNTKETNMPYRYQFLAEDFDTQRDINEIDIACEHINLSL
QDNQIPYARFIKDLKTRLEYSIINNIPISEEDYQDYLNLSGSQQTINEEELLKLVNTEVKSQDMEGQVLS
EQEMKINIYLKESQKEAIELVKVINGRLADENSADMVIKTINSQILDTPFIQSLINDSIAIHNVQESKDQ
IMILEQQLANAYELAVFKTKNSILKNKIIKLKNAMTKLAKAI
MKLVQNNEVKEIKNGFVKNSNKILGKLKKVGSFKKNKYHTGKIEGEDLPKSLQDKLNVPLVFIGVFLGSI
VFLAITYLLNFLKALLVAIGQSESGLLGVEFDPKFSLAYLKPLGDTKQYFMCAVVAVIGVAYLIYGKLNH
ESEKNLVYGQKGDSRLSTIKEIKRDYHEIPEKTEVYDGIGGVPISHYQDKYYIDRDTVNTVVLGASRSGK
GETFVVPLLDNLSRAKIQSNMVVNDPKGELFSASKETLEKRGYRVLVLNIDDPLESMSFNPLQLVIDSWA
NKDYHEASKRANTLTSMLFASGMGTDNEFFYKSAKSAVNAIILTIVEHCFNNDCIEKITMYNVAQMLNEL
GSLFYTDPKTGKEKNALDEYFNTLPQGNQAKIQYGSTSFAGDKAKGSILSTASQGIEMFTSDLFGKLTSK
MSIDLKEIGFPKSIQFKVNTNLVGKRISISFLRKENDVIRLIKKYRVKVKALGLCVLNFNEYLQDGDMLD
IRYEEEKSRAWYRIEFPKKQANSHDTSLVTKKFKSGKNYSDLEISTLRLKYTDQPTAVFMVIPDYDPSNH
VLASIFISQLYTELASNCKQTPDKKCFRRVHFLLDEFGNMPAIDNMDGIMTVCLGRNMLFDLVIQSYSQL
ETRYDKAFKTIKENCQNHILIMSNDEETIEEISKKCGHKTTINRSASSKHLDTDSSVTSSAEQERVITVE
RASQLIEGEQIILRNLHRQDTKRNKIRPFPIFNTKETNMPYRYQFLAEDFDTQRDINEIDIACEHINLSL
QDNQIPYARFIKDLKTRLEYSIINNIPISEEDYQDYLNLSGSQQTINEEELLKLVNTEVKSQDMEGQVLS
EQEMKINIYLKESQKEAIELVKVINGRLADENSADMVIKTINSQILDTPFIQSLINDSIAIHNVQESKDQ
IMILEQQLANAYELAVFKTKNSILKNKIIKLKNAMTKLAKAI
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 1631..21292
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| JCP89_RS14880 (JCP89_14875) | 56..952 | - | 897 | WP_002311863 | hypothetical protein | - |
| JCP89_RS14885 (JCP89_14880) | 966..1616 | - | 651 | WP_002311862 | cell division protein FtsL | - |
| JCP89_RS14890 (JCP89_14885) | 1631..2767 | - | 1137 | WP_002311861 | bifunctional lytic transglycosylase/C40 family peptidase | orf14 |
| JCP89_RS14895 (JCP89_14890) | 2789..3109 | - | 321 | WP_010729568 | hypothetical protein | virb4 |
| JCP89_RS14900 (JCP89_14895) | 3297..5174 | - | 1878 | WP_002305177 | group II intron reverse transcriptase/maturase | - |
| JCP89_RS14905 (JCP89_14900) | 5862..7487 | - | 1626 | WP_233097250 | VirB4 family type IV secretion system protein | virb4 |
| JCP89_RS14910 (JCP89_14905) | 7503..7970 | - | 468 | WP_002311859 | hypothetical protein | - |
| JCP89_RS14915 (JCP89_14910) | 7986..8603 | - | 618 | WP_002311858 | hypothetical protein | virb4 |
| JCP89_RS14920 (JCP89_14915) | 8600..8911 | - | 312 | WP_002317413 | DUF5592 family protein | - |
| JCP89_RS14925 (JCP89_14920) | 8959..11628 | - | 2670 | WP_002326868 | pLS20_p028 family conjugation system transmembrane protein | - |
| JCP89_RS14930 (JCP89_14925) | 11646..14504 | - | 2859 | WP_002311855 | VirD4-like conjugal transfer protein, CD1115 family | - |
| JCP89_RS14935 (JCP89_14930) | 14517..15233 | - | 717 | WP_002311854 | hypothetical protein | - |
| JCP89_RS14940 (JCP89_14935) | 15246..17072 | - | 1827 | WP_002311853 | hypothetical protein | - |
| JCP89_RS14945 (JCP89_14940) | 17181..17456 | - | 276 | WP_002311852 | TrbC/VirB2 family protein | virB2 |
| JCP89_RS14950 (JCP89_14945) | 17475..17696 | - | 222 | WP_002326867 | hypothetical protein | - |
| JCP89_RS14955 | 17708..17854 | - | 147 | WP_002326866 | hypothetical protein | - |
| JCP89_RS14960 (JCP89_14950) | 17868..18230 | - | 363 | WP_002311851 | DUF4320 family protein | - |
| JCP89_RS14965 (JCP89_14955) | 18248..19045 | - | 798 | WP_002311850 | hypothetical protein | - |
| JCP89_RS14970 (JCP89_14960) | 19045..19860 | - | 816 | WP_002313315 | hypothetical protein | - |
| JCP89_RS14975 (JCP89_14965) | 19877..21292 | - | 1416 | WP_002311848 | CpaF/VirB11 family protein | virB11 |
| JCP89_RS14980 (JCP89_14970) | 21304..21726 | - | 423 | WP_002311847 | hypothetical protein | - |
| JCP89_RS14985 (JCP89_14975) | 21794..21946 | - | 153 | WP_002311846 | hypothetical protein | - |
| JCP89_RS14990 (JCP89_14980) | 21960..22322 | - | 363 | WP_002311845 | hypothetical protein | - |
| JCP89_RS14995 (JCP89_14985) | 22382..23167 | - | 786 | WP_046669026 | hypothetical protein | - |
| JCP89_RS15000 (JCP89_14990) | 23317..24543 | + | 1227 | WP_046650365 | IS21 family transposase | - |
| JCP89_RS15005 (JCP89_14995) | 24558..25313 | + | 756 | WP_010818485 | IS21-like element helper ATPase IstB | - |
Host bacterium
| ID | 22094 | GenBank | NZ_CP066436 |
| Plasmid name | p66_65kb | Incompatibility group | - |
| Plasmid size | 65409 bp | Coordinate of oriT [Strand] | 64937..65128 [+] |
| Host baterium | Enterococcus faecium strain Dallas 66 |
Cargo genes
| Drug resistance gene | dfrG |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA1 |