Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 121666
• Name: oriT_pB2801
• Organism: Bacillus siamensis strain B28
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP066220 (5441..5463 [+], 23 nt)
• oriT length: 23 nt
• IRs (inverted repeats): 2..7, 17..22 (CCCCCC..GGGGGG) 1..6, 17..22 (CCCCCC..GGGGGG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 23 nt

>oriT_pB2801
CCCCCCCATGCTAACAGGGGGGT

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_JD965_RS20115_pB2801

• ID: 13667
• GenBank: WP_198698999
• Name: mobV_JD965_RS20115_pB2801
• UniProt ID: _
• Length: 483 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 483 a.a.
• Molecular weight: 56802.39 Da
• Isoelectric Point: 9.7332

>WP_198698999.1 MULTISPECIES: MobV family relaxase [Bacillus]
MANYAVIRMEKYKKDRLNGTQKHNQREFQKSKNENIDRGRSHLNYDLVNGQPISYAQAVREKIEGRVKRK
VRDDAVLVSEFLITASPDYMNGLSETEQRRYFEAAVDHLKEKYTAENMLYATVHMDEATPHMHVGIVPIT
EDGRLSAKDFFNGKLKMKAIQDDFHRHMVKSGFDLERGEPSEKKHENVHQYKINQRKAELERLNAEIALK
EKRKAELEQQNRAVESVVSVKKEALTAKMQEQRIPTIEYEKAGLFKKDKVSVPQHELENLYAYAEDQGKM
AAQLSRQLEAETEEKERWKSIAVEERERADSKDKMLSDLQKAMNEEVEKAKRGMRRKLTKEFTEEQREEL
RQEVKEELSALKTENSELRTENNDLKRLTLSLSAQKDNEVKKHAETKKTLKTERGRYSELLNFAKEQNKE
FKSLNEENKTLRERVAVLEQWKDKMVQWAKEKLPRVRKLAASFFRTAGMPREANKYKDHELER

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-192)

Protein structure

No available structure.

Host bacterium

• ID: 22093
• GenBank: NZ_CP066220
• Plasmid name: pB2801
• Incompatibility group: Col
• Plasmid size: 6117 bp
• Coordinate of oriT [Strand]: 5441..5463 [+]
• Host baterium: Bacillus siamensis strain B28

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -