Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   121662
Name   oriT_pARO1.2 in_silico
Organism   Enterococcus faecalis ARO1/DG
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP022485 (55333..55382 [+], 50 nt)
oriT length   50 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 50 nt

>oriT_pARO1.2
AATATCGCAACATGGTACCATGTTGCTCTGCTCGCAAAAAGAAAGCCTAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   13664 GenBank   WP_002367697
Name   Relaxase_CG806_RS00735_pARO1.2 insolico UniProt ID   _
Length   561 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 561 a.a.        Molecular weight: 65315.04 Da        Isoelectric Point: 6.1225

>WP_002367697.1 relaxase/mobilization nuclease domain-containing protein [Enterococcus faecalis]
MVYTKHFVIHTFDKLNNACSYIENAEKTEVTNDNPSEHLEHLFQYIVNDDKTYMKKLVSGHGIVDPTNPY
EEFKLTKLQAAIQRKIGYTFDPKSERLLPPTLTELEKGNAVLAHHLIQSFSPEDDLTPEKIHEIGYNTVM
ELTGGEYEFVIATHVDKEHLHNHIIFSSTNLKTGKAFRWQKGTKKVFEQISDKIAAKEGAKIIEKSPKNS
HKKYTMWETESLYKEKIKSRLDFLLEQSSSINDFLEKAEALNLSVDFSKKWTTYRLLDEPQIKNTRSRSL
SKSDPTRYNYEKIVERLKENKSVLTLKEVVEKYGEKSEKSKNEFDYQLTIDEWQISHKTTRGYYVNVDFG
FGERGKVFIGAYKVDPLENGQYNIYVKRKDFFYLMNEKNADRNRYMTGETLIKQLRLYNGQTPLKKEPVM
QTIDELVNAINFLAANEIDDTRQLELLEEKLEAAFLEAEETLETLDEKMLELHQLSNLLLENELNGELEL
IQQKLKTLLPDATLAEFSYKDVRGEIEAIKTSQSLLENKLERTRNEINQLHEIQAVQKKETANQNNVKPK
L

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(37-296)


  Protein structure



No available structure.




Auxiliary protein

ID   7529 GenBank   WP_002367696
Name   WP_002367696_pARO1.2 insolico UniProt ID   Q82YL4
Length   118 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 118 a.a.        Molecular weight: 14042.10 Da        Isoelectric Point: 9.9724

>WP_002367696.1 MULTISPECIES: plasmid mobilization relaxosome protein MobC [Bacteria]
MENKQKLKRPIQRKIRFSKEENELINRKIEESFFPNFQNFALHLLIQGEIRHVDYSELNRLTTEIHKIGI
NINQMARLANQFHEISSEDIKDLTDKVQSLNALVQSELNKLIKRKEQS

  Protein domains


Predicted by InterproScan.

Bacterial mobilisation protein (MobC) (MobC)

(64-105)


  Protein structure


Source ID Structure
AlphaFold DB Q82YL4


T4CP

ID   15970 GenBank   WP_002367691
Name   t4cp2_CG806_RS00715_pARO1.2 insolico UniProt ID   _
Length   609 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 609 a.a.        Molecular weight: 70145.14 Da        Isoelectric Point: 6.1807

>WP_002367691.1 VirD4-like conjugal transfer protein, CD1115 family [Enterococcus faecalis]
MQTYKKELKPYLYLGIFLFVIGFGIGNFFWLLPGTDYFSKTNYLFTKDILTYIEQTFYRFFITPSLLGFS
VGILGFLLGLLMYVRDNDRGIYRHGEEYGSARFATPAEMKKYEDPIPENNIIVSKHVKISLFNKRLPIKL
QKNKNIAILGDSGAAKTLAFIKTNLMQRHASFITTDPDGGILPEIGLLLKKGKYKIKVLDLNTLSNSDTF
NVFEYMHSELDVDRVLEAVTEATKEVEKTTGDDFWIKAEGLLVRSLIAYLWFDGRRNDYTPHLGMIADML
RHLKRKDKNVPSPVEEWFEELNEAIPDNYAYRQWTLFNNLYESETRASVLGIAAARYSVFDHEEVVNMIR
TDTMDIDSWNEEKTAVFIAIPETNTSFNFIAALMFATVGEVLRNKSDQVRLGKRALEEGKELLHVRFLID
EFANIGRIPHFEKMLTTFRKREMSFTIVLQSLNQLQTLYRNGWQNILNGCACLLYLGGDEEETTKYLSAR
AGKQTLSIRKHSMNKGNQGGGSENRDKYGRDLIDRSEVALIGGDECLVFISKEHVFKDKKFYAFQHDRAD
ELATGPTDDKWYTWRRYMTDEEAILDKISATDIIDHGEITSELKEETHV

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(144-558)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 33951..52688

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CG806_RS00600 (CG806_00585) 29134..29514 + 381 WP_002367664 hypothetical protein -
CG806_RS00610 (CG806_00595) 30085..30390 + 306 WP_002367666 hypothetical protein -
CG806_RS00615 (CG806_00600) 30401..33073 + 2673 WP_002367667 surface exclusion protein SEA1/PrgA -
CG806_RS00620 (CG806_00605) 33139..33726 + 588 WP_002367669 hypothetical protein -
CG806_RS00625 (CG806_00610) 33951..37865 + 3915 WP_002367670 LPXTG-anchored aggregation substance PrgB/Asc10 prgB
CG806_RS00630 (CG806_00615) 37927..38283 + 357 WP_002367672 pheromone response system RNA-binding regulator PrgU -
CG806_RS00635 (CG806_00620) 38311..39168 + 858 WP_002367673 pheromone response LPXTG-anchored adhesin PrgC prgC
CG806_RS00640 (CG806_00625) 39209..40108 + 900 WP_025186739 hypothetical protein -
CG806_RS00645 (CG806_00630) 40128..40562 + 435 WP_002365857 hypothetical protein -
CG806_RS00650 (CG806_00635) 40609..40836 + 228 WP_002365858 hypothetical protein -
CG806_RS00655 (CG806_00640) 40849..41145 + 297 WP_002367677 hypothetical protein -
CG806_RS00660 (CG806_00645) 41160..41960 + 801 WP_002367679 type IV secretion system protein prgHb
CG806_RS00665 (CG806_00650) 41962..42315 + 354 WP_002365862 PrgI family mobile element protein prgIc
CG806_RS00670 (CG806_00655) 42269..44659 + 2391 WP_002365863 VirB4-like conjugal transfer ATPase, CD1110 family virb4
CG806_RS00675 (CG806_00660) 44671..47286 + 2616 WP_002367681 phage tail tip lysozyme prgK
CG806_RS00680 (CG806_00665) 47310..47936 + 627 WP_002367683 hypothetical protein prgL
CG806_RS00685 (CG806_00670) 47923..48168 + 246 WP_002365866 hypothetical protein -
CG806_RS00690 (CG806_00675) 48152..48760 + 609 WP_002367686 hypothetical protein -
CG806_RS00695 (CG806_00680) 48874..49629 + 756 WP_002367687 Fic family protein -
CG806_RS00700 (CG806_00685) 49646..50104 + 459 WP_002367688 single-stranded DNA-binding protein -
CG806_RS00705 (CG806_00690) 50150..50332 + 183 WP_002367689 hypothetical protein -
CG806_RS00710 (CG806_00695) 50374..50859 + 486 WP_002386493 PcfB family protein gbs1365
CG806_RS00715 (CG806_00700) 50859..52688 + 1830 WP_002367691 VirD4-like conjugal transfer protein, CD1115 family virb4
CG806_RS00720 (CG806_00705) 52739..54898 + 2160 WP_002367693 ArdC-like ssDNA-binding domain-containing protein -
CG806_RS00725 (CG806_00710) 54931..55203 + 273 WP_002367695 LtrD -
CG806_RS00730 (CG806_00715) 55449..55805 + 357 WP_002367696 plasmid mobilization relaxosome protein MobC -
CG806_RS00735 (CG806_00720) 55806..57491 + 1686 WP_002367697 relaxase/mobilization nuclease domain-containing protein -

Host bacterium

ID   22089 GenBank   NZ_CP022485
Plasmid name   pARO1.2 Incompatibility group   -
Plasmid size   59380 bp Coordinate of oriT [Strand]   55333..55382 [+]
Host baterium   Enterococcus faecalis ARO1/DG

Cargo genes

Drug resistance gene   -
Virulence gene   prgB/asc10
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA9