Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 121658 |
| Name | oriT_pAtLMG292b  |
| Organism | Agrobacterium tumefaciens strain LMG 292 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP116691 (64976..65017 [+], 42 nt) |
| oriT length | 42 nt |
| IRs (inverted repeats) | 22..27, 31..36 (CGTCGT..ACGACG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 42 nt
>oriT_pAtLMG292b
GGTTCCAAGGGCGCAATTATACGTCGTGATACGACGCGTTGC
GGTTCCAAGGGCGCAATTATACGTCGTGATACGACGCGTTGC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 13662 | GenBank | WP_080830924 |
| Name | traA_G6L31_RS24765_pAtLMG292b |
UniProt ID | _ |
| Length | 1100 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1100 a.a. Molecular weight: 123748.22 Da Isoelectric Point: 9.9274
>WP_080830924.1 MULTISPECIES: Ti-type conjugative transfer relaxase TraA [Agrobacterium]
MAIAHFSASIVSRGSGRSVVLSAAYRHCAKMEFEREARTIDYTRKQGLLHEEFVLPANAPEWARSLIADR
SVSGAIEAFWNKVEAFEKRADAQLARDLTIALPLELSAEQNIALVRDFVEKHILAKGMVADWVYHENPGN
PHIHLMTTLRPLSDESFGSKKVAVIGEDGQPVRTKSGKILYELWAGSTDDFNVLRDGWFERLNHHLALGG
IDLKIDGRSYEKQGINLEPTVHLGVGAKAIERKAEQRGVRPELERVELNEQRRSENTRRILNNPAIVLDL
ITREKSVFDERDIAKVLHRYIDDPALFQQLMLKIILNPDVLRLQRETIDFSTGEKLPARYSTRAMIRLEA
TMARQATWLSNREGRSVSPTALDATFRRHEQLSDEQKAAIERVAGPARIAAVVGRAGAGKTTMMKAAREV
WELAGYHVVGGALAGKAAEGLEKEAGIQSRTLASWELRWKRGRDLLDDKTIFIMDEAGMVASKQMAAFVD
AAVRAGAKIVLVGDPEQLQPIEAGAAFRAIVDRIGYAELETIYRQRAEWMRKASLDLARGNVENALSAYG
ANVRIAGERLKAEAVERLIADWNHDYDQTKTILILAHLRRDVRMLNVMAREKLVERGIVGEGHLFRTADG
ERRFDAGDQIVFLKNERSLGVKNGMIGHVVEAAANRIVATVGEGDRRRQVIVEQRFYNNLDHGYATTIHK
SQGATVDRVKVLASLSLDRHLTYVAMTRHREDLQLYYGTRSFSFNGGLAKVLSRRQAKETTLDYEHGQFY
REALRFAETRGLHVVQVARTMVRDRLDWTLRQKAKLVDLSRRLAAFAAHLGITQSPKTQTMKETAPMVAG
IKIFSGSVADTVGDRLGTDPALKQQWEEVSARFAYVFADPESAFRAINFDAVLADKEVANQVLRKIEVEP
ASIGPLKGKTDILATRSEREARRVAEVNVPALKRDLEQYLRMRETVTQRLQTDEQSLRQRVSIDIPALSP
GARVVLERVRDAIDRNDLPAAMAYAISNRETKLEIDGFNRAVTERFGERTLLSNSAREPSGKLYEKLSES
MKPEQKEQLKQAWPVMRTAQQLAAHERTVQSLRQAEEQRLTQRQTPVLKQ
MAIAHFSASIVSRGSGRSVVLSAAYRHCAKMEFEREARTIDYTRKQGLLHEEFVLPANAPEWARSLIADR
SVSGAIEAFWNKVEAFEKRADAQLARDLTIALPLELSAEQNIALVRDFVEKHILAKGMVADWVYHENPGN
PHIHLMTTLRPLSDESFGSKKVAVIGEDGQPVRTKSGKILYELWAGSTDDFNVLRDGWFERLNHHLALGG
IDLKIDGRSYEKQGINLEPTVHLGVGAKAIERKAEQRGVRPELERVELNEQRRSENTRRILNNPAIVLDL
ITREKSVFDERDIAKVLHRYIDDPALFQQLMLKIILNPDVLRLQRETIDFSTGEKLPARYSTRAMIRLEA
TMARQATWLSNREGRSVSPTALDATFRRHEQLSDEQKAAIERVAGPARIAAVVGRAGAGKTTMMKAAREV
WELAGYHVVGGALAGKAAEGLEKEAGIQSRTLASWELRWKRGRDLLDDKTIFIMDEAGMVASKQMAAFVD
AAVRAGAKIVLVGDPEQLQPIEAGAAFRAIVDRIGYAELETIYRQRAEWMRKASLDLARGNVENALSAYG
ANVRIAGERLKAEAVERLIADWNHDYDQTKTILILAHLRRDVRMLNVMAREKLVERGIVGEGHLFRTADG
ERRFDAGDQIVFLKNERSLGVKNGMIGHVVEAAANRIVATVGEGDRRRQVIVEQRFYNNLDHGYATTIHK
SQGATVDRVKVLASLSLDRHLTYVAMTRHREDLQLYYGTRSFSFNGGLAKVLSRRQAKETTLDYEHGQFY
REALRFAETRGLHVVQVARTMVRDRLDWTLRQKAKLVDLSRRLAAFAAHLGITQSPKTQTMKETAPMVAG
IKIFSGSVADTVGDRLGTDPALKQQWEEVSARFAYVFADPESAFRAINFDAVLADKEVANQVLRKIEVEP
ASIGPLKGKTDILATRSEREARRVAEVNVPALKRDLEQYLRMRETVTQRLQTDEQSLRQRVSIDIPALSP
GARVVLERVRDAIDRNDLPAAMAYAISNRETKLEIDGFNRAVTERFGERTLLSNSAREPSGKLYEKLSES
MKPEQKEQLKQAWPVMRTAQQLAAHERTVQSLRQAEEQRLTQRQTPVLKQ
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 15966 | GenBank | WP_080830930 |
| Name | traG_G6L31_RS24750_pAtLMG292b |
UniProt ID | _ |
| Length | 646 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 646 a.a. Molecular weight: 69204.28 Da Isoelectric Point: 9.3374
>WP_080830930.1 MULTISPECIES: Ti-type conjugative transfer system protein TraG [Agrobacterium]
MTANRLLLTILPAAIMIAALVMMSGLEHRLAALGTSAPARLALGRAGLVLPYVGAAAIGVVALFATHGST
NIKAAGLSVLAGSAVVVIIAMTREAIRLAAIASDVPAGQSVLAYADPATMLGAAVAFIGSVFALRVAIKG
NAAFAMAAPKRIGGKRAVHGEADWMKLPEAAKVFPEAGGIVIGERYRVDRDSVATMPFRSDEPQSWGAGG
KSPLLCFDGSFGSSHGIVFAGSGGFKTTSVTIPTALKWGGGLVVLDPSSEVAPMVIEHRRKAGRKVIVLD
PTASGVGLNALDWIGRHGNTKEEDIVAVATWIMTDNAHTASARDDFFRASAMQLLTALIADVCLSGHTDE
KEQTLRQVRANLSEPEPKLRARLTKIYEGSDSDFVKENVSVFVNMTPETFSGVYANAVKETHWLSYRNYA
GLVSGDSFSTDDLANGETDIFIALDLKVLEAHPGFARVVIGSLLNAIYNRNGDVKGRTLFLLDEVARLGY
LRILETARDAGRKYGITLTMIFQSLGQMREAYGGRDATSKWFESASWISFAAINDPDTADYISKRCGDTT
VEVDQTNRSTGMKGSSRSRSKQLNRRPLILPHEVLRMRADEQIVFTAGNAPLRCGRAVWFRREDMKACVG
ENRFHKNSSGTDSPGR
MTANRLLLTILPAAIMIAALVMMSGLEHRLAALGTSAPARLALGRAGLVLPYVGAAAIGVVALFATHGST
NIKAAGLSVLAGSAVVVIIAMTREAIRLAAIASDVPAGQSVLAYADPATMLGAAVAFIGSVFALRVAIKG
NAAFAMAAPKRIGGKRAVHGEADWMKLPEAAKVFPEAGGIVIGERYRVDRDSVATMPFRSDEPQSWGAGG
KSPLLCFDGSFGSSHGIVFAGSGGFKTTSVTIPTALKWGGGLVVLDPSSEVAPMVIEHRRKAGRKVIVLD
PTASGVGLNALDWIGRHGNTKEEDIVAVATWIMTDNAHTASARDDFFRASAMQLLTALIADVCLSGHTDE
KEQTLRQVRANLSEPEPKLRARLTKIYEGSDSDFVKENVSVFVNMTPETFSGVYANAVKETHWLSYRNYA
GLVSGDSFSTDDLANGETDIFIALDLKVLEAHPGFARVVIGSLLNAIYNRNGDVKGRTLFLLDEVARLGY
LRILETARDAGRKYGITLTMIFQSLGQMREAYGGRDATSKWFESASWISFAAINDPDTADYISKRCGDTT
VEVDQTNRSTGMKGSSRSRSKQLNRRPLILPHEVLRMRADEQIVFTAGNAPLRCGRAVWFRREDMKACVG
ENRFHKNSSGTDSPGR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 15967 | GenBank | WP_080831044 |
| Name | t4cp2_G6L31_RS25170_pAtLMG292b |
UniProt ID | _ |
| Length | 822 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 822 a.a. Molecular weight: 91808.73 Da Isoelectric Point: 6.0652
>WP_080831044.1 MULTISPECIES: conjugal transfer protein TrbE [Agrobacterium]
MVALKSFRHSGPSFADLVPYAGLVDNGVVLLKDGSLMAGWYFAGPDSESSTDAERNEVSRQINAILSRLG
SGWMIQVEAVRVPTGDYPTEDASHFPDPVTRVIDAERRAHFQKERGHYESRHALILTWRPPEPRRSGLTR
YVYSDAGSRSATYADKAIETFLTSIREVEQYLANVVSIRRMMTRETPERGGHRVARYDELFQFIRFCITG
ENHPVRLPQIPMYLDWLVTAELQHGLTPLVENRFLGVVAIDGLPAESWPGILNSLDLMPLTYRWSSRFVF
LDAEEARARLERTRKKWQQKVRPFFDQLFQTQSRSLDQDAMMMVAETEDAIAEASSQLVAYGYYTPVIVL
FDEDQSRLQEKCEAVRRLIQAEGFGARIETINATDAFLGSLPGVSYANIREPLINTRNLADLIPLNSIWS
GSPIAPCPFYPVGSPPLMQVASGSTPFRLNLHVDDVGHTLIFGPTGSGKSTLLSLIAAQFRRYAGAQIFA
FDKGGSMLPLTLGLDGDHYQIGGDLGPSANGQARTLSFCPLAELSTDGDRAFASEWIEMLVALQGVTITP
DYRNAISRQIGLMAESRGRSISDFVSGVQMREIKDALHHYTVDGPMGQLLDAEEDGLALGAFQCFEIEEL
MNMGERNLVPVLTYLFRRIEKRLTGVPSLIILDEAWLMLGHPVFRDKIREWLKVLRKANCAVVLATQSIS
DAERSGIIDVLKESCPTKICLPNGAAREPGTREFYERIGFNERQIEIVATAIPKRDYYVVSPEGRRLFDM
GLGPVALSFVGASGKEDLKRIRALHLEHGASWPLHWLQQRGIAHADTLFPTQ
MVALKSFRHSGPSFADLVPYAGLVDNGVVLLKDGSLMAGWYFAGPDSESSTDAERNEVSRQINAILSRLG
SGWMIQVEAVRVPTGDYPTEDASHFPDPVTRVIDAERRAHFQKERGHYESRHALILTWRPPEPRRSGLTR
YVYSDAGSRSATYADKAIETFLTSIREVEQYLANVVSIRRMMTRETPERGGHRVARYDELFQFIRFCITG
ENHPVRLPQIPMYLDWLVTAELQHGLTPLVENRFLGVVAIDGLPAESWPGILNSLDLMPLTYRWSSRFVF
LDAEEARARLERTRKKWQQKVRPFFDQLFQTQSRSLDQDAMMMVAETEDAIAEASSQLVAYGYYTPVIVL
FDEDQSRLQEKCEAVRRLIQAEGFGARIETINATDAFLGSLPGVSYANIREPLINTRNLADLIPLNSIWS
GSPIAPCPFYPVGSPPLMQVASGSTPFRLNLHVDDVGHTLIFGPTGSGKSTLLSLIAAQFRRYAGAQIFA
FDKGGSMLPLTLGLDGDHYQIGGDLGPSANGQARTLSFCPLAELSTDGDRAFASEWIEMLVALQGVTITP
DYRNAISRQIGLMAESRGRSISDFVSGVQMREIKDALHHYTVDGPMGQLLDAEEDGLALGAFQCFEIEEL
MNMGERNLVPVLTYLFRRIEKRLTGVPSLIILDEAWLMLGHPVFRDKIREWLKVLRKANCAVVLATQSIS
DAERSGIIDVLKESCPTKICLPNGAAREPGTREFYERIGFNERQIEIVATAIPKRDYYVVSPEGRRLFDM
GLGPVALSFVGASGKEDLKRIRALHLEHGASWPLHWLQQRGIAHADTLFPTQ
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 145121..154806
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| G6L31_RS25110 (G6L31_025110) | 140157..141011 | + | 855 | WP_080830805 | alpha/beta fold hydrolase | - |
| G6L31_RS25115 (G6L31_025115) | 141135..141578 | + | 444 | WP_080830803 | PaaI family thioesterase | - |
| G6L31_RS25120 (G6L31_025120) | 141866..142699 | - | 834 | WP_173991204 | SDR family oxidoreductase | - |
| G6L31_RS25125 (G6L31_025125) | 142780..144198 | - | 1419 | WP_137066522 | coniferyl aldehyde dehydrogenase | - |
| G6L31_RS25130 (G6L31_025130) | 144293..144943 | + | 651 | WP_080831087 | TetR/AcrR family transcriptional regulator | - |
| G6L31_RS25135 (G6L31_025135) | 145121..146425 | - | 1305 | WP_080831059 | IncP-type conjugal transfer protein TrbI | virB10 |
| G6L31_RS25140 (G6L31_025140) | 146437..146916 | - | 480 | WP_080831056 | conjugal transfer protein TrbH | - |
| G6L31_RS25145 (G6L31_025145) | 146916..147770 | - | 855 | WP_137066524 | P-type conjugative transfer protein TrbG | virB9 |
| G6L31_RS25150 (G6L31_025150) | 147788..148450 | - | 663 | WP_080831085 | conjugal transfer protein TrbF | virB8 |
| G6L31_RS25155 (G6L31_025155) | 148468..149658 | - | 1191 | WP_173991202 | P-type conjugative transfer protein TrbL | virB6 |
| G6L31_RS25160 (G6L31_025160) | 149652..149885 | - | 234 | WP_080831049 | entry exclusion protein TrbK | - |
| G6L31_RS25165 (G6L31_025165) | 149882..150691 | - | 810 | WP_080831047 | P-type conjugative transfer protein TrbJ | virB5 |
| G6L31_RS25170 (G6L31_025170) | 150663..153131 | - | 2469 | WP_080831044 | conjugal transfer protein TrbE | virb4 |
| G6L31_RS25175 (G6L31_025175) | 153143..153442 | - | 300 | WP_080831041 | conjugal transfer protein TrbD | virB3 |
| G6L31_RS25180 (G6L31_025180) | 153435..153845 | - | 411 | WP_080831038 | conjugal transfer pilin TrbC | virB2 |
| G6L31_RS25185 (G6L31_025185) | 153835..154806 | - | 972 | WP_080831036 | P-type conjugative transfer ATPase TrbB | virB11 |
| G6L31_RS25190 (G6L31_025190) | 154803..155438 | - | 636 | WP_080831034 | acyl-homoserine-lactone synthase | - |
Host bacterium
| ID | 22085 | GenBank | NZ_CP116691 |
| Plasmid name | pAtLMG292b | Incompatibility group | - |
| Plasmid size | 155815 bp | Coordinate of oriT [Strand] | 64976..65017 [+] |
| Host baterium | Agrobacterium tumefaciens strain LMG 292 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA11a.5 |