Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 121649 |
| Name | oriT_2022CK-00499|unnamed2  |
| Organism | Klebsiella oxytoca strain 2022CK-00499 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP114310 (43605..43709 [+], 105 nt) |
| oriT length | 105 nt |
| IRs (inverted repeats) | 32..37, 40..45 (GCCGGC..GCCGGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 105 nt
>oriT_2022CK-00499|unnamed2
AGTACGGGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT
AGTACGGGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 13657 | GenBank | WP_004206924 |
| Name | traI_OEE41_RS29380_2022CK-00499|unnamed2 |
UniProt ID | A0A2V4FJ40 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75087.84 Da Isoelectric Point: 10.0285
>WP_004206924.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFAGIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQLRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFAGIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQLRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2V4FJ40 |
T4CP
| ID | 15961 | GenBank | WP_004206885 |
| Name | t4cp2_OEE41_RS29130_2022CK-00499|unnamed2 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79538.91 Da Isoelectric Point: 4.8317
>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 46507..65703
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| OEE41_RS29360 (OEE41_29355) | 41591..41902 | + | 312 | WP_004187333 | hypothetical protein | - |
| OEE41_RS29365 (OEE41_29360) | 42035..42571 | + | 537 | WP_004206920 | hypothetical protein | - |
| OEE41_RS29370 (OEE41_29365) | 43072..43437 | - | 366 | WP_004206922 | hypothetical protein | - |
| OEE41_RS29375 (OEE41_29370) | 43713..44030 | + | 318 | WP_004206923 | plasmid mobilization protein MobA | - |
| OEE41_RS29380 (OEE41_29375) | 44017..45996 | + | 1980 | WP_004206924 | TraI/MobA(P) family conjugative relaxase | - |
| OEE41_RS29385 (OEE41_29380) | 46010..46510 | + | 501 | WP_004206925 | DotD/TraH family lipoprotein | - |
| OEE41_RS29390 (OEE41_29385) | 46507..47286 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| OEE41_RS29395 (OEE41_29390) | 47297..48460 | + | 1164 | WP_004206926 | plasmid transfer ATPase TraJ | virB11 |
| OEE41_RS29400 (OEE41_29395) | 48450..48710 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| OEE41_RS29405 (OEE41_29400) | 48735..52040 | + | 3306 | WP_219818010 | LPD7 domain-containing protein | - |
| OEE41_RS29410 (OEE41_29405) | 52006..52518 | + | 513 | WP_011091071 | hypothetical protein | traL |
| OEE41_RS29415 (OEE41_29410) | 52519..52731 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| OEE41_RS29420 (OEE41_29415) | 52703..53113 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| OEE41_RS29425 (OEE41_29420) | 53181..53894 | + | 714 | WP_223294958 | DotI/IcmL family type IV secretion protein | traM |
| OEE41_RS29430 (OEE41_29425) | 53903..55054 | + | 1152 | WP_015062843 | DotH/IcmK family type IV secretion protein | traN |
| OEE41_RS29435 (OEE41_29430) | 55066..56415 | + | 1350 | WP_004206932 | conjugal transfer protein TraO | traO |
| OEE41_RS29440 (OEE41_29435) | 56427..57131 | + | 705 | WP_004206933 | conjugal transfer protein TraP | traP |
| OEE41_RS29445 (OEE41_29440) | 57155..57685 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| OEE41_RS29450 (OEE41_29445) | 57702..58091 | + | 390 | WP_011154472 | DUF6750 family protein | traR |
| OEE41_RS29455 (OEE41_29450) | 58137..58631 | + | 495 | WP_004187480 | hypothetical protein | - |
| OEE41_RS29460 (OEE41_29455) | 58628..61678 | + | 3051 | WP_011154474 | conjugative transfer protein | traU |
| OEE41_RS29465 (OEE41_29460) | 61675..62883 | + | 1209 | WP_004187486 | conjugal transfer protein TraW | traW |
| OEE41_RS29470 (OEE41_29465) | 62880..63530 | + | 651 | WP_004187488 | hypothetical protein | - |
| OEE41_RS29475 (OEE41_29470) | 63523..65703 | + | 2181 | WP_004187492 | DotA/TraY family protein | traY |
| OEE41_RS29480 (OEE41_29475) | 65706..66359 | + | 654 | WP_004206935 | hypothetical protein | - |
| OEE41_RS29485 (OEE41_29480) | 66433..66663 | + | 231 | WP_004187496 | IncL/M type plasmid replication protein RepC | - |
Host bacterium
| ID | 22076 | GenBank | NZ_CP114310 |
| Plasmid name | 2022CK-00499|unnamed2 | Incompatibility group | IncL/M |
| Plasmid size | 66959 bp | Coordinate of oriT [Strand] | 43605..43709 [+] |
| Host baterium | Klebsiella oxytoca strain 2022CK-00499 |
Cargo genes
| Drug resistance gene | blaKPC-2 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |