Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 121642 |
| Name | oriT_2022CK-00567|unnamed3  |
| Organism | Klebsiella variicola strain 2022CK-00567 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP114357 (43544..43648 [+], 105 nt) |
| oriT length | 105 nt |
| IRs (inverted repeats) | 32..37, 40..45 (GCCGGC..GCCGGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 105 nt
>oriT_2022CK-00567|unnamed3
AGTACGGGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT
AGTACGGGACAAGATGTGTTTTTGGTACACCGCCGGCACGCCGGCAGTGACGCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTAT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 13651 | GenBank | WP_269776270 |
| Name | traI_OPU51_RS29210_2022CK-00567|unnamed3 |
UniProt ID | _ |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75118.85 Da Isoelectric Point: 10.0285
>WP_269776270.1 TraI/MobA(P) family conjugative relaxase [Klebsiella variicola]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFAGIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQLRSESDDKFNQK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFAGIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQLRSESDDKFNQK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 15957 | GenBank | WP_004206885 |
| Name | t4cp2_OPU51_RS28960_2022CK-00567|unnamed3 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79538.91 Da Isoelectric Point: 4.8317
>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 46446..65642
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| OPU51_RS29190 (OPU51_29190) | 41530..41841 | + | 312 | WP_004187333 | hypothetical protein | - |
| OPU51_RS29195 (OPU51_29195) | 41974..42510 | + | 537 | WP_004206920 | hypothetical protein | - |
| OPU51_RS29200 (OPU51_29200) | 43011..43376 | - | 366 | WP_004206922 | hypothetical protein | - |
| OPU51_RS29205 (OPU51_29205) | 43652..43969 | + | 318 | WP_004206923 | plasmid mobilization protein MobA | - |
| OPU51_RS29210 (OPU51_29210) | 43956..45935 | + | 1980 | WP_269776270 | TraI/MobA(P) family conjugative relaxase | - |
| OPU51_RS29215 (OPU51_29215) | 45949..46449 | + | 501 | WP_004206925 | DotD/TraH family lipoprotein | - |
| OPU51_RS29220 (OPU51_29220) | 46446..47225 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| OPU51_RS29225 (OPU51_29225) | 47236..48399 | + | 1164 | WP_004206926 | plasmid transfer ATPase TraJ | virB11 |
| OPU51_RS29230 (OPU51_29230) | 48389..48649 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| OPU51_RS29235 (OPU51_29235) | 48674..51979 | + | 3306 | WP_219818010 | LPD7 domain-containing protein | - |
| OPU51_RS29240 (OPU51_29240) | 51945..52457 | + | 513 | WP_011091071 | hypothetical protein | traL |
| OPU51_RS29245 (OPU51_29245) | 52458..52670 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| OPU51_RS29250 (OPU51_29250) | 52642..53052 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| OPU51_RS29255 (OPU51_29255) | 53120..53833 | + | 714 | WP_223294958 | DotI/IcmL family type IV secretion protein | traM |
| OPU51_RS29260 (OPU51_29260) | 53842..54993 | + | 1152 | WP_015062843 | DotH/IcmK family type IV secretion protein | traN |
| OPU51_RS29265 (OPU51_29265) | 55005..56354 | + | 1350 | WP_004206932 | conjugal transfer protein TraO | traO |
| OPU51_RS29270 (OPU51_29270) | 56366..57070 | + | 705 | WP_004206933 | conjugal transfer protein TraP | traP |
| OPU51_RS29275 (OPU51_29275) | 57094..57624 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| OPU51_RS29280 (OPU51_29280) | 57641..58030 | + | 390 | WP_011154472 | DUF6750 family protein | traR |
| OPU51_RS29285 (OPU51_29285) | 58076..58570 | + | 495 | WP_004187480 | hypothetical protein | - |
| OPU51_RS29290 (OPU51_29290) | 58567..61617 | + | 3051 | WP_011154474 | conjugative transfer protein | traU |
| OPU51_RS29295 (OPU51_29295) | 61614..62822 | + | 1209 | WP_004187486 | conjugal transfer protein TraW | traW |
| OPU51_RS29300 (OPU51_29300) | 62819..63469 | + | 651 | WP_004187488 | hypothetical protein | - |
| OPU51_RS29305 (OPU51_29305) | 63462..65642 | + | 2181 | WP_004187492 | DotA/TraY family protein | traY |
| OPU51_RS29310 (OPU51_29310) | 65645..66298 | + | 654 | WP_004206935 | hypothetical protein | - |
| OPU51_RS29315 (OPU51_29315) | 66372..66602 | + | 231 | WP_004187496 | IncL/M type plasmid replication protein RepC | - |
Host bacterium
| ID | 22069 | GenBank | NZ_CP114357 |
| Plasmid name | 2022CK-00567|unnamed3 | Incompatibility group | IncL/M |
| Plasmid size | 66898 bp | Coordinate of oriT [Strand] | 43544..43648 [+] |
| Host baterium | Klebsiella variicola strain 2022CK-00567 |
Cargo genes
| Drug resistance gene | ant(2'')-Ia, blaSHV-30, qnrS1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |