Detailed information of oriT
oriT
The information of the oriT region
oriTDB ID | 121636 |
Name | oriT_2022CK-00505|unnamed1 |
Organism | Klebsiella variicola strain 2022CK-00505 |
Sequence Completeness | - |
NCBI accession of oriT (coordinates [strand]) | NZ_CP114333 (193250..193299 [+], 50 nt) |
oriT length | 50 nt |
IRs (inverted repeats) | 7..14, 17..24 (GCAAAATT..AATTTTGC) |
Location of nic site | 33..34 |
Conserved sequence flanking the nic site |
GGTGTGGTGA |
Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 50 nt
>oriT_2022CK-00505|unnamed1
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
AAATCTGCAAAATTTTAATTTTGCGTGGGGTGTGGTGATTTTGTGGTGAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure fileRelaxase
ID | 13647 | GenBank | WP_269136661 |
Name | traI_OGU18_RS27745_2022CK-00505|unnamed1 | UniProt ID | _ |
Length | 1753 a.a. | PDB ID | |
Note | Predicted by oriTfinder 2.0 |
Relaxase protein sequence
Download Length: 1753 a.a. Molecular weight: 190450.44 Da Isoelectric Point: 6.2888
>WP_269136661.1 conjugative transfer relaxase/helicase TraI [Klebsiella variicola]
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANRHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFDLARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRLVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGQQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDRDGVRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEDGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKTRSGETDLDAAIAQQKAGLRTPAEQAIHLAIPLLESEKLTFSRPQLLATALETGGGKVPMADIDTTI
QAQIRSGQLLNVPVAHGHGNDLLISRQTWDAEKSILTHVLEGKDAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQR
SAADVAIMKEIVRQVPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGVWAPGSSVVEFTPAQEKAIQ
KALSEGETLPAGQPATLYEALVKDYTGRTPEAQSQTLIITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERYISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDLERGYVANSIWEVQSVSGDSVTLSDGKTTRTLTPKADQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIKHSPEKATAHDILE
PRNDRAVKSADLLFGRARPLDETAAGRAALQQSGLAQGNSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNDAARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAITGGRVWADPAPVGPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGPEKTTLPDDPQFRRQEAAIQQVASESLQRERLQAVERDMVRDLNREKTL
GGD
MMSIGSVKSAGSAGNYYTDKDNYYVIGSMDERWQGKGAEALGIDGKAVDKALFTELLKGKLPDGSDLTRI
QDGANRHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVTEAVRQLETLAATRVMTDGKSETVLTGNL
IVAKFNHDTNRNQEPQIHTHAVVINATQNGDKWQSLGTDKIGKTGFIENVYANQIAFGKLYREAFKPLVE
KLGYETEVVGKHGMWEMKGVPVEPFSTRSQEVREAAGPDASLKSRDVAALDTRKSKEAIDPAEKMVEWMN
TLKETGFDIRGYREAADARAAELARAPAAPVNTDGPDITDVVTKAIAGLSDRKVQFTYADLLARTVGQLE
AKDGVFDLARKGIDAAIEREQLIPLDREKGLFTSNIHVLDELAVKALSQEVQRQNHVSVTPDASVVRQVP
FSDAVSVLAQDRLVMGIVSGQGGATGQRERVAELTLMAREQGRDVHILAADNRSRDFLAGDVRLAGETVT
GKSALQDGTAFIPGGTLIVDQAEKLSLKETISLLDGAMRHNVQVLLSDSGKRSGTGSALTVLKDSGVNTY
RWQGGQQTTADIISEPDKGARYSRLAQEFAVSVREGQESVAQISGTREQSVLNGLIRDSLRQEGVLGEKD
TTITALTPVWLDSKSRGVRDYYREGMVMERWDPENRTHDRFVIDRVTASSNMLTLKDRDGVRLDLKVSAV
DSQWTLFRAETLPVAEGERLAVLGKIPDTRLKGGESITVMKVEDGQLTVQRPGQKTTQTLAVGAGVFDGI
KIGHGWVESPGRSVSETATVFASVTQRELDNATLNQLAQSGSHLRLYSAQDAARTTEKLSRHTAFSVVSE
QLKTRSGETDLDAAIAQQKAGLRTPAEQAIHLAIPLLESEKLTFSRPQLLATALETGGGKVPMADIDTTI
QAQIRSGQLLNVPVAHGHGNDLLISRQTWDAEKSILTHVLEGKDAVAPLMDRVPASLMTDLTAGQRAATR
MILESTDRFTVVQGYAGVGKTTQFRAVMSAISLLPEETRPRVIGLAPTHRAVGEMQSAGVDARTTASFLH
DTQLLQRNGQTPDFSNTLFLLDESSMVGLADMAKAHSLIVAGGGRAVSSGDNDQLQPIAPGQPFRLMQQR
SAADVAIMKEIVRQVPELRPAVYSLIERDVHRALTTIEQVTPEQVPRKEGVWAPGSSVVEFTPAQEKAIQ
KALSEGETLPAGQPATLYEALVKDYTGRTPEAQSQTLIITHLNKDRRALNSLIHDARRENGETGKEEITL
PVLVTSNIRDGELRKLSTWTAHKEAVALVDNVYHRISKVDKDNQLITLTDSEGKERYISPREASAEGVTL
YRQEKITVSQGDRMRFSKSDLERGYVANSIWEVQSVSGDSVTLSDGKTTRTLTPKADQAQQHIDLAYAIT
AHGAQGASEPYAIALEGVAGGREQMASFESAYVALSRMKQHVQVYTDSREGWIKAIKHSPEKATAHDILE
PRNDRAVKSADLLFGRARPLDETAAGRAALQQSGLAQGNSPGKFISPGKKYPQPHVALPAFDKNGKAAGI
WLSPLTDRDGRLEAIGGEGRIMGNDAARFVALQNSRNGESLLAGNMGEGVRMARDNPDTGVVVRLAGDDR
PWNPGAITGGRVWADPAPVGPVPQAGADIILPPEVLAQRAAEEQQRREMEKQAEQTAREVAGEARKAGEP
ADRVKEVIGDVIRGLERDRPGPEKTTLPDDPQFRRQEAAIQQVASESLQRERLQAVERDMVRDLNREKTL
GGD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
ID | 15952 | GenBank | WP_095835928 |
Name | traD_OGU18_RS27750_2022CK-00505|unnamed1 | UniProt ID | _ |
Length | 770 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
T4CP protein sequence
Download Length: 770 a.a. Molecular weight: 85922.99 Da Isoelectric Point: 5.1149
>WP_095835928.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Klebsiella]
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADITVSPAPVKAPPTTKMPAEEPSVRSTEPSALRLTTVPLIKPKAAAAAAAAATVSSAGTPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDNVEIGGNF
MSFNAKDMTQGGQIANMRFRMFGQIANIIFYVLFILFWVLCGLMLMYRLSWQTFVNGCVYWWCTTLGPMR
DIIRSQPVYTIQYYGQSLEYTSEQILADKYTIWCGEQLWTSFVFAAVVSLVICIVTFFIASWVLGRQGKQ
QSEDENTGGRQLSDKPKEVARQMKRDGMASDIKIGDLPILKNSEIQNFCLHGTVGSGKSEVIRRLLNYVR
ARGDMAIIYDRSCEFVKSYYDPSLDKILNPLDSRCAAWDLWKECLTLPDFDNISNTLIPMGTKEDPFWQG
SGRTIFAEGAYLMREDKDRSYEKLVDTMLSIKIDKLRAYLQNTPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEKNGEPFTIRDWMRGVREDRPNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WIFADELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGVKPAATLFDVMNTRAFFRSPSKEIA
EFAAGEIGEKEILKASEQYSYGADPVRDGVSTGKEKERETLVSYSDIQTLPDLSCYVTLPGPYPAVKLAL
KYKPRPKIAEGFIPRTLDARVDARLSALLEAREAEGSLARALFTPDAPASGPADTNSHAGEQPEPVSQPA
PADITVSPAPVKAPPTTKMPAEEPSVRSTEPSALRLTTVPLIKPKAAAAAAAAATVSSAGTPAAAAGGTE
QELAQQSAEQGQDMLPAGMNEDGVIEDMQAYDAWLADEQTQRDMQRREEVNINHSHRHDEQDNVEIGGNF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 164058..190752
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
OGU18_RS27750 (OGU18_27745) | 164058..166370 | - | 2313 | WP_095835928 | type IV conjugative transfer system coupling protein TraD | virb4 |
OGU18_RS27755 (OGU18_27750) | 166730..167461 | - | 732 | WP_032415736 | conjugal transfer complement resistance protein TraT | - |
OGU18_RS27760 (OGU18_27755) | 167713..168315 | - | 603 | WP_023280878 | hypothetical protein | - |
OGU18_RS27765 (OGU18_27760) | 168449..169429 | + | 981 | WP_000019445 | IS5-like element ISKpn26 family transposase | - |
OGU18_RS27770 (OGU18_27765) | 169530..172361 | - | 2832 | WP_269136662 | conjugal transfer mating-pair stabilization protein TraG | traG |
OGU18_RS27775 (OGU18_27770) | 172361..173740 | - | 1380 | WP_011977731 | conjugal transfer pilus assembly protein TraH | traH |
OGU18_RS27780 (OGU18_27775) | 173718..174161 | - | 444 | WP_015065638 | F-type conjugal transfer protein TrbF | - |
OGU18_RS27785 (OGU18_27780) | 174206..174748 | - | 543 | WP_013054815 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
OGU18_RS27790 (OGU18_27785) | 174735..174962 | - | 228 | WP_114506536 | type-F conjugative transfer system pilin chaperone TraQ | - |
OGU18_RS27795 (OGU18_27790) | 174973..175725 | - | 753 | WP_015065637 | type-F conjugative transfer system pilin assembly protein TraF | traF |
OGU18_RS27800 (OGU18_27795) | 175746..176072 | - | 327 | WP_032436744 | hypothetical protein | - |
OGU18_RS29465 | 176118..176303 | - | 186 | WP_032436745 | hypothetical protein | - |
OGU18_RS27805 (OGU18_27800) | 176300..176536 | - | 237 | WP_032436746 | conjugal transfer protein TrbE | - |
OGU18_RS27810 (OGU18_27805) | 176568..178523 | - | 1956 | WP_023307537 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
OGU18_RS27815 (OGU18_27810) | 178582..179220 | - | 639 | WP_015065635 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
OGU18_RS27820 (OGU18_27815) | 179233..180192 | - | 960 | WP_015065634 | conjugal transfer pilus assembly protein TraU | traU |
OGU18_RS27825 (OGU18_27820) | 180236..180862 | - | 627 | WP_023307538 | type-F conjugative transfer system protein TraW | traW |
OGU18_RS27830 (OGU18_27825) | 180862..181251 | - | 390 | WP_004152591 | type-F conjugative transfer system protein TrbI | - |
OGU18_RS27835 (OGU18_27830) | 181251..183890 | - | 2640 | WP_016528865 | type IV secretion system protein TraC | virb4 |
OGU18_RS27840 (OGU18_27835) | 183962..184360 | - | 399 | WP_072158599 | hypothetical protein | - |
OGU18_RS27845 (OGU18_27840) | 184368..184658 | - | 291 | WP_023307542 | hypothetical protein | - |
OGU18_RS27850 (OGU18_27845) | 184655..185059 | - | 405 | WP_023307543 | hypothetical protein | - |
OGU18_RS27855 (OGU18_27850) | 185126..185443 | - | 318 | WP_023307544 | hypothetical protein | - |
OGU18_RS27860 (OGU18_27855) | 185444..185662 | - | 219 | WP_004171484 | hypothetical protein | - |
OGU18_RS27865 (OGU18_27860) | 185686..185976 | - | 291 | WP_032423381 | hypothetical protein | - |
OGU18_RS27870 (OGU18_27865) | 185981..186391 | - | 411 | WP_269136663 | hypothetical protein | - |
OGU18_RS27875 (OGU18_27870) | 186523..187092 | - | 570 | WP_023307547 | type IV conjugative transfer system lipoprotein TraV | traV |
OGU18_RS27880 (OGU18_27875) | 187304..187717 | - | 414 | Protein_194 | conjugal transfer pilus-stabilizing protein TraP | - |
OGU18_RS27885 (OGU18_27880) | 187710..189134 | - | 1425 | WP_023307549 | F-type conjugal transfer pilus assembly protein TraB | traB |
OGU18_RS27890 (OGU18_27885) | 189134..189874 | - | 741 | WP_004152497 | type-F conjugative transfer system secretin TraK | traK |
OGU18_RS27895 (OGU18_27890) | 189861..190427 | - | 567 | WP_004152602 | type IV conjugative transfer system protein TraE | traE |
OGU18_RS27900 (OGU18_27895) | 190447..190752 | - | 306 | WP_004178059 | type IV conjugative transfer system protein TraL | traL |
OGU18_RS27905 (OGU18_27900) | 190766..191134 | - | 369 | WP_023307550 | type IV conjugative transfer system pilin TraA | - |
OGU18_RS27910 (OGU18_27905) | 191188..191559 | - | 372 | WP_004208838 | TraY domain-containing protein | - |
OGU18_RS27915 (OGU18_27910) | 191643..192338 | - | 696 | WP_023307551 | transcriptional regulator TraJ family protein | - |
OGU18_RS27920 (OGU18_27915) | 192545..192937 | - | 393 | WP_004206766 | conjugal transfer relaxosome DNA-binding protein TraM | - |
OGU18_RS27925 (OGU18_27920) | 193444..193851 | + | 408 | WP_020805750 | transglycosylase SLT domain-containing protein | - |
OGU18_RS27930 (OGU18_27925) | 193889..194419 | - | 531 | WP_015632491 | antirestriction protein | - |
Host bacterium
ID | 22063 | GenBank | NZ_CP114333 |
Plasmid name | 2022CK-00505|unnamed1 | Incompatibility group | IncFIB |
Plasmid size | 227788 bp | Coordinate of oriT [Strand] | 193250..193299 [+] |
Host baterium | Klebsiella variicola strain 2022CK-00505 |
Cargo genes
Drug resistance gene | - |
Virulence gene | - |
Metal resistance gene | silE, silS, silR, silC, silF, silB, silA, silP, pcoA, pcoB, pcoC, pcoD, pcoR, pcoS, pcoE, arsC, arsB, arsA, arsD, arsR, arsH |
Degradation gene | - |
Symbiosis gene | - |
Anti-CRISPR | AcrIE9 |