Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 121629 |
| Name | oriT_8月1日|unnamed1  |
| Organism | Klebsiella michiganensis strain 8-1 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP089449 (58189..58294 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_8月1日|unnamed1
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 13643 | GenBank | WP_050868819 |
| Name | traI_LT930_RS28165_8月1日|unnamed1 |
UniProt ID | A0A142BMF1 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75234.98 Da Isoelectric Point: 10.0285
>WP_050868819.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPVYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSERDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPVYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSERDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A142BMF1 |
T4CP
| ID | 15947 | GenBank | WP_011091092 |
| Name | t4cp2_LT930_RS27880_8月1日|unnamed1 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79491.81 Da Isoelectric Point: 4.8270
>WP_011091092.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFADWLDGTDTDDVLYHQEDDLFPMIEM
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFADWLDGTDTDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 61090..80193
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LT930_RS28145 (LT930_28145) | 56174..56485 | + | 312 | WP_011091065 | hypothetical protein | - |
| LT930_RS28150 (LT930_28150) | 56618..57154 | + | 537 | WP_011091066 | hypothetical protein | - |
| LT930_RS28155 (LT930_28155) | 57655..58020 | - | 366 | WP_011091067 | hypothetical protein | - |
| LT930_RS28160 (LT930_28160) | 58086..58613 | + | 528 | WP_225986328 | plasmid mobilization protein MobA | - |
| LT930_RS28165 (LT930_28165) | 58600..60579 | + | 1980 | WP_050868819 | TraI/MobA(P) family conjugative relaxase | - |
| LT930_RS28170 (LT930_28170) | 60593..61093 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| LT930_RS28175 (LT930_28175) | 61090..61869 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| LT930_RS28180 (LT930_28180) | 61880..63043 | + | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| LT930_RS28185 (LT930_28185) | 63033..63293 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| LT930_RS28190 (LT930_28190) | 63318..66530 | + | 3213 | WP_011091070 | LPD7 domain-containing protein | - |
| LT930_RS28195 (LT930_28195) | 66496..67008 | + | 513 | WP_011091071 | hypothetical protein | traL |
| LT930_RS28200 (LT930_28200) | 67009..67221 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| LT930_RS28205 (LT930_28205) | 67193..67603 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| LT930_RS28210 (LT930_28210) | 67671..68384 | + | 714 | WP_109914255 | DotI/IcmL family type IV secretion protein | traM |
| LT930_RS28215 (LT930_28215) | 68393..69544 | + | 1152 | WP_011091074 | DotH/IcmK family type IV secretion protein | traN |
| LT930_RS28220 (LT930_28220) | 69556..70905 | + | 1350 | WP_011091075 | conjugal transfer protein TraO | traO |
| LT930_RS28225 (LT930_28225) | 70917..71621 | + | 705 | WP_011091076 | conjugal transfer protein TraP | traP |
| LT930_RS28230 (LT930_28230) | 71645..72175 | + | 531 | WP_011091077 | conjugal transfer protein TraQ | traQ |
| LT930_RS28235 (LT930_28235) | 72192..72581 | + | 390 | WP_011091078 | DUF6750 family protein | traR |
| LT930_RS28240 (LT930_28240) | 72627..73121 | + | 495 | WP_011091080 | hypothetical protein | - |
| LT930_RS28245 (LT930_28245) | 73118..76168 | + | 3051 | WP_011091081 | hypothetical protein | traU |
| LT930_RS28250 (LT930_28250) | 76165..77373 | + | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| LT930_RS28255 (LT930_28255) | 77370..78020 | + | 651 | WP_011091083 | hypothetical protein | - |
| LT930_RS28260 (LT930_28260) | 78013..80193 | + | 2181 | WP_004187492 | DotA/TraY family protein | traY |
| LT930_RS28265 (LT930_28265) | 80196..80849 | + | 654 | WP_011091084 | hypothetical protein | - |
| LT930_RS28270 (LT930_28270) | 80924..81154 | + | 231 | WP_011091085 | IncL/M type plasmid replication protein RepC | - |
Host bacterium
| ID | 22056 | GenBank | NZ_CP089449 |
| Plasmid name | 8月1日|unnamed1 | Incompatibility group | IncL/M |
| Plasmid size | 81451 bp | Coordinate of oriT [Strand] | 58189..58294 [+] |
| Host baterium | Klebsiella michiganensis strain 8-1 |
Cargo genes
| Drug resistance gene | blaTEM-1B, blaKPC-2, blaCTX-M-3 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |