Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 121610 |
| Name | oriT_2014C-4356|unnamed5  |
| Organism | Escherichia albertii strain 2014C-4356 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP024287 (1408..1530 [+], 123 nt) |
| oriT length | 123 nt |
| IRs (inverted repeats) | 100..105, 118..123 (TTTAAT..ATTAAA) 90..98, 112..120 (AATAATGTA..TACATTATT) 89..94, 106..111 (AAATAA..TTATTT) 38..45, 48..55 (GCAAAAAC..GTTTTTGC) 2..9, 14..21 (TTGGTGGT..ACCACCAA) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 123 nt
>oriT_2014C-4356|unnamed5
GTTGGTGGTTCTCACCACCAAAAGCACCACACCCCACGCAAAAACAAGTTTTTGCTGATTTGCTTTTTGAATCATTAGCTTATGTTTTAAATAATGTATTTTAATTTATTTTACATTATTAAA
GTTGGTGGTTCTCACCACCAAAAGCACCACACCCCACGCAAAAACAAGTTTTTGCTGATTTGCTTTTTGAATCATTAGCTTATGTTTTAAATAATGTATTTTAATTTATTTTACATTATTAAA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 13630 | GenBank | WP_099588277 |
| Name | traI_CCF14_RS27295_2014C-4356|unnamed5 |
UniProt ID | _ |
| Length | 1756 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 1756 a.a. Molecular weight: 192043.78 Da Isoelectric Point: 5.9263
>WP_099588277.1 conjugative transfer relaxase/helicase TraI [Escherichia albertii]
MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAEWMQT
LKETGFDIRAYRDAADQRAETRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVADSPFTALKL
ENGWVETPGHSVSDSAKVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETSLETAISHQKSALHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTSFVDLGREIDA
QIKRGDLLHVDVAKGYGTDLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMI
LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRLEGAWAPEHSVMEFSHSQEAKLAEA
QQKAMQNGGAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNCMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWETHRDALALVDNVYHWIAGISKDDGLITLQDAEGNTRLISPREAVAEGVT
LYTPDTIRVGTGDRMRFTKSDRECGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPARERAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGSRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVL
EPKSDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGRTEQAVREIAGQERERAVTSEREAALPESVLREPQREREAVREVARENLLQERLQQMERDMVRDLQKE
KTLGGD
MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGRLPDGADLSRMQ
DGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLV
MALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEA
LGYETEVVGKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAEWMQT
LKETGFDIRAYRDAADQRAETRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPE
NGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGR
RQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRW
QGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVT
MTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDS
SWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVADSPFTALKL
ENGWVETPGHSVSDSAKVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETSLETAISHQKSALHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTSFVDLGREIDA
QIKRGDLLHVDVAKGYGTDLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMI
LETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDT
QLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSA
ADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRLEGAWAPEHSVMEFSHSQEAKLAEA
QQKAMQNGGAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNCMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWETHRDALALVDNVYHWIAGISKDDGLITLQDAEGNTRLISPREAVAEGVT
LYTPDTIRVGTGDRMRFTKSDRECGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPARERAEQHIDLAYAI
TAHGAQGASETFAIALEGTEGSRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVL
EPKSDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAG
IWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGRTEQAVREIAGQERERAVTSEREAALPESVLREPQREREAVREVARENLLQERLQQMERDMVRDLQKE
KTLGGD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
| ID | 7515 | GenBank | WP_000124981 |
| Name | WP_000124981_2014C-4356|unnamed5 |
UniProt ID | A0A630FTW9 |
| Length | 127 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 127 a.a. Molecular weight: 14542.59 Da Isoelectric Point: 5.0278
>WP_000124981.1 MULTISPECIES: conjugal transfer relaxosome DNA-binding protein TraM [Gammaproteobacteria]
MARVNLYISNEVHEKINMIVEKRRQEGARDKDISLSGTASMLLELGLRVYDAQMERKESAFNQTEFNKLL
LECVVKTQSTVAKILGIESLSPHVSGNPKFEYASMVDDIREKVSVEMDRFFPKNDDE
MARVNLYISNEVHEKINMIVEKRRQEGARDKDISLSGTASMLLELGLRVYDAQMERKESAFNQTEFNKLL
LECVVKTQSTVAKILGIESLSPHVSGNPKFEYASMVDDIREKVSVEMDRFFPKNDDE
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A630FTW9 |
| ID | 7516 | GenBank | WP_001254386 |
| Name | WP_001254386_2014C-4356|unnamed5 |
UniProt ID | A0A3Z6KJB5 |
| Length | 75 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 75 a.a. Molecular weight: 9005.11 Da Isoelectric Point: 10.1422
>WP_001254386.1 MULTISPECIES: conjugal transfer relaxosome protein TraY [Gammaproteobacteria]
MRRRNARGGISRTVSVYLDEDTNNRLIKAKDRSGRSKTIEVQIRLRDHLKRFPDFYNEEIFREVTEESES
TFKEL
MRRRNARGGISRTVSVYLDEDTNNRLIKAKDRSGRSKTIEVQIRLRDHLKRFPDFYNEEIFREVTEESES
TFKEL
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3Z6KJB5 |
T4CP
| ID | 15933 | GenBank | WP_001360340 |
| Name | traD_CCF14_RS27290_2014C-4356|unnamed5 |
UniProt ID | _ |
| Length | 729 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 729 a.a. Molecular weight: 82860.46 Da Isoelectric Point: 5.0524
>WP_001360340.1 MULTISPECIES: type IV conjugative transfer system coupling protein TraD [Enterobacteriaceae]
MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLVLWVKISWQTFVNGCIYWWCTTLEGMR
DLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQ
QSENEVTGGRQLTENPKDVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYAR
QRGDMVVIYDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIA
EFAAGEIGEKEHLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL
KYQARPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQPQQPQQ
PQQPQQPVSPAINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAAYEAWQQENHPD
IQQQMQRREEVNINVHRERGEDVEPGDDF
MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLVLWVKISWQTFVNGCIYWWCTTLEGMR
DLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQ
QSENEVTGGRQLTENPKDVARMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYAR
QRGDMVVIYDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQG
SGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIEKTAISIRAVLTNYVKA
IRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGLLAMGENRNRRV
WFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIA
EFAAGEIGEKEHLKASEQYSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSL
KYQARPKVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQPQQPQQ
PQQPQQPVSPAINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAAYEAWQQENHPD
IQQQMQRREEVNINVHRERGEDVEPGDDF
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 3753..28793
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| CCF14_RS27125 | 835..1404 | - | 570 | Protein_1 | transglycosylase SLT domain-containing protein | - |
| CCF14_RS27130 | 1759..2142 | + | 384 | WP_000124981 | conjugal transfer relaxosome DNA-binding protein TraM | - |
| CCF14_RS27135 | 2332..3018 | + | 687 | WP_000332496 | PAS domain-containing protein | - |
| CCF14_RS27140 | 3112..3339 | + | 228 | WP_001254386 | conjugal transfer relaxosome protein TraY | - |
| CCF14_RS27145 | 3373..3738 | + | 366 | WP_089634697 | type IV conjugative transfer system pilin TraA | - |
| CCF14_RS27150 | 3753..4064 | + | 312 | WP_000012106 | type IV conjugative transfer system protein TraL | traL |
| CCF14_RS27155 | 4086..4652 | + | 567 | WP_000399792 | type IV conjugative transfer system protein TraE | traE |
| CCF14_RS27160 | 4639..5367 | + | 729 | WP_052923301 | type-F conjugative transfer system secretin TraK | traK |
| CCF14_RS27165 | 5367..6796 | + | 1430 | Protein_9 | F-type conjugal transfer pilus assembly protein TraB | - |
| CCF14_RS27170 | 6786..7376 | + | 591 | WP_000002778 | conjugal transfer pilus-stabilizing protein TraP | - |
| CCF14_RS27175 | 7363..7560 | + | 198 | WP_001324648 | conjugal transfer protein TrbD | - |
| CCF14_RS27180 | 7572..7823 | + | 252 | WP_001038342 | conjugal transfer protein TrbG | - |
| CCF14_RS27185 | 7820..8335 | + | 516 | WP_089634700 | type IV conjugative transfer system lipoprotein TraV | traV |
| CCF14_RS27190 | 8470..8691 | + | 222 | WP_099588274 | conjugal transfer protein TraR | - |
| CCF14_RS27195 | 8851..9837 | + | 987 | Protein_15 | TraC family protein | - |
| CCF14_RS27200 | 9869..11440 | - | 1572 | WP_000381395 | IS66-like element ISCro1 family transposase | - |
| CCF14_RS27205 | 11460..11807 | - | 348 | WP_000624622 | IS66 family insertion sequence element accessory protein TnpB | - |
| CCF14_RS27210 | 11807..12484 | - | 678 | WP_001682408 | IS66-like element accessory protein TnpA | - |
| CCF14_RS27215 | 12539..14185 | + | 1647 | Protein_19 | type IV secretion system protein TraC | - |
| CCF14_RS27220 | 14182..14568 | + | 387 | WP_000214082 | type-F conjugative transfer system protein TrbI | - |
| CCF14_RS27225 | 14565..15197 | + | 633 | WP_001203720 | type-F conjugative transfer system protein TraW | traW |
| CCF14_RS27230 | 15194..16186 | + | 993 | WP_000830839 | conjugal transfer pilus assembly protein TraU | traU |
| CCF14_RS27235 | 16195..16833 | + | 639 | WP_000777695 | type-F conjugative transfer system pilin assembly protein TrbC | trbC |
| CCF14_RS27240 | 16830..18638 | + | 1809 | WP_000821844 | type-F conjugative transfer system mating-pair stabilization protein TraN | traN |
| CCF14_RS27245 | 18665..18922 | + | 258 | WP_122988881 | conjugal transfer protein TrbE | - |
| CCF14_RS27250 | 18915..19658 | + | 744 | WP_001030371 | type-F conjugative transfer system pilin assembly protein TraF | traF |
| CCF14_RS27255 | 19819..20055 | + | 237 | WP_001346259 | type-F conjugative transfer system pilin chaperone TraQ | - |
| CCF14_RS27260 | 20042..20587 | + | 546 | WP_000052618 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | traF |
| CCF14_RS27265 | 20517..20879 | + | 363 | WP_002430925 | P-type conjugative transfer protein TrbJ | - |
| CCF14_RS27270 | 20879..22252 | + | 1374 | WP_170981808 | conjugal transfer pilus assembly protein TraH | traH |
| CCF14_RS27275 | 22249..25071 | + | 2823 | WP_099588275 | conjugal transfer mating-pair stabilization protein TraG | traG |
| CCF14_RS27280 | 25086..25589 | + | 504 | WP_099588276 | surface exclusion protein | - |
| CCF14_RS27285 | 25621..26352 | + | 732 | WP_001447147 | conjugal transfer complement resistance protein TraT | - |
| CCF14_RS27290 | 26604..28793 | + | 2190 | WP_001360340 | type IV conjugative transfer system coupling protein TraD | virb4 |
Host bacterium
| ID | 22037 | GenBank | NZ_CP024287 |
| Plasmid name | 2014C-4356|unnamed5 | Incompatibility group | - |
| Plasmid size | 124142 bp | Coordinate of oriT [Strand] | 1408..1530 [+] |
| Host baterium | Escherichia albertii strain 2014C-4356 |
Cargo genes
| Drug resistance gene | aph(6)-Id, aph(3'')-Ib |
| Virulence gene | - |
| Metal resistance gene | arsA, arsB, arsC, arsH |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |