Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   121601
Name   oriT_pTT39_p3 in_silico
Organism   Enterococcus faecium strain K60-39
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP023426 (25124..25146 [+], 23 nt)
oriT length   23 nt
IRs (inverted repeats)      1..7, 14..20  (AAGTATA..TATACTT)
Location of nic site      12..13
Conserved sequence flanking the
  nic site    		  GTGTGTTATA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 23 nt

>oriT_pTT39_p3
AAGTATAGTGTGTTATACTTTAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   13622 GenBank   WP_000119405
Name   mobV_CNX66_RS15675_pTT39_p3 insolico UniProt ID   F5X340
Length   420 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 420 a.a.        Molecular weight: 49660.33 Da        Isoelectric Point: 9.9693

>WP_000119405.1 MULTISPECIES: MobV family relaxase [Bacteria]
MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYDLKNDKNIDYNERVKEIIESQKTGTR
KTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPM
RDGKLQGKNVFNRQELLWLQDKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVK
KDEWTAYSDKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDN
DRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSKISDLKRDVSL
IYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNEFELTHNREVKKERSRDQGMSL

  Protein domains


Predicted by InterproScan.

Plasmid recombination enzyme (Mob_Pre)

(1-193)


  Protein structure


Source ID Structure
AlphaFold DB F5X340


Host bacterium

ID   22028 GenBank   NZ_CP023426
Plasmid name   pTT39_p3 Incompatibility group   -
Plasmid size   40017 bp Coordinate of oriT [Strand]   25124..25146 [+]
Host baterium   Enterococcus faecium strain K60-39

Cargo genes

Drug resistance gene   lsa(E), lnu(B), ant(6)-Ia, aph(3')-III, erm(B)
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -