Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   121600
Name   oriT_pT212 in_silico
Organism   Proteus mirabilis strain T21
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP017084 (75628..75778 [-], 151 nt)
oriT length   151 nt
IRs (inverted repeats)      93..98, 106..111  (TGGCCT..AGGCCA)
 12..17, 24..29  (AACCCT..AGGGTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 151 nt

>oriT_pT212
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   13621 GenBank   WP_001337757
Name   mobH_BG257_RS19910_pT212 insolico UniProt ID   A0A709FRI2
Length   992 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 992 a.a.        Molecular weight: 109581.85 Da        Isoelectric Point: 4.8956

>WP_001337757.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEADDGDDQEEGEAALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGPTESSK
PDAGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPF
DAFNASAETTSTDATNSEIPDVAMPGKQEEQPKQDFVPQEQNSLQGDDFPMFGGSDEPPSWAIEPLPMLT
DAPEQPTHTPEMPHTDNVNQHEKDAKTLLVEMLSGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVI
LYPDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQ
DAFELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKASPEQKAKGKDSQPQPKEKKVDVTSPVEEQQ
RKPVQEKQNVARLPKREAQPVAPEPKVEREKELGHVEVREREDPEVREFEPPKAKTNPKDINAEDFLPSG
VTPQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKK
RQGKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure


Source ID Structure
AlphaFold DB A0A709FRI2


T4CP

ID   15921 GenBank   WP_000637386
Name   traC_BG257_RS19830_pT212 insolico UniProt ID   _
Length   815 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 815 a.a.        Molecular weight: 92590.87 Da        Isoelectric Point: 5.9222

>WP_000637386.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIVTIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSAGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTTLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTERIFAKTNKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQQLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSKGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRQQAA

  Protein domains


Predicted by InterproScan.

TraG P-loop domain (P-loop_TraG)

(449-809)

Family of unknown function (DUF5934) (DUF5934)

(284-434)

TraC protein (TraC_F_IV)

(21-255)

  Protein structure



No available structure.



ID   15922 GenBank   WP_000178856
Name   traD_BG257_RS19905_pT212 insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69197.66 Da        Isoelectric Point: 6.9403

>WP_000178856.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPDDMWLGSGFLWENRHAQRVFEILKRDWTSIVGRESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVMPDWEAEAAAYLEKAKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-297)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 52503..77694

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG257_RS19770 (BG257_19370) 47630..48538 - 909 WP_000739139 hypothetical protein -
BG257_RS19775 (BG257_19375) 48549..49517 - 969 WP_000085162 AAA family ATPase -
BG257_RS19780 (BG257_19380) 49737..49922 - 186 WP_001186917 hypothetical protein -
BG257_RS19785 (BG257_19385) 50226..50546 - 321 WP_000547566 hypothetical protein -
BG257_RS19790 (BG257_19390) 50840..51481 + 642 WP_000796664 hypothetical protein -
BG257_RS19795 (BG257_19395) 51604..52464 + 861 WP_000709517 hypothetical protein -
BG257_RS19800 (BG257_19400) 52503..55301 - 2799 WP_001256487 conjugal transfer mating pair stabilization protein TraN traN
BG257_RS19805 (BG257_19405) 55417..56421 - 1005 WP_043940352 TraU family protein traU
BG257_RS19810 (BG257_19410) 56421..57092 - 672 WP_001337754 EAL domain-containing protein -
BG257_RS19815 (BG257_19415) 57089..58354 - 1266 WP_000621288 TrbC family F-type conjugative pilus assembly protein traW
BG257_RS19820 (BG257_19420) 58317..58847 - 531 WP_001010738 S26 family signal peptidase -
BG257_RS19825 (BG257_19425) 58844..59161 - 318 WP_000351984 hypothetical protein -
BG257_RS19830 (BG257_19430) 59176..61623 - 2448 WP_000637386 type IV secretion system protein TraC virb4
BG257_RS19835 (BG257_19435) 61620..62327 - 708 WP_001259347 DsbC family protein -
BG257_RS19840 (BG257_19440) 62476..68007 - 5532 WP_085281193 Ig-like domain-containing protein -
BG257_RS19845 (BG257_19445) 68208..68600 - 393 WP_000479535 TraA family conjugative transfer protein -
BG257_RS19850 (BG257_19450) 68604..69182 - 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
BG257_RS19855 (BG257_19455) 69179..70492 - 1314 WP_024131605 TraB/VirB10 family protein traB
BG257_RS19860 (BG257_19460) 70492..71409 - 918 WP_096043119 type-F conjugative transfer system secretin TraK traK
BG257_RS19865 (BG257_19465) 71393..72019 - 627 WP_001049716 TraE/TraK family type IV conjugative transfer system protein traE
BG257_RS19870 (BG257_19470) 72016..72297 - 282 WP_000805625 type IV conjugative transfer system protein TraL traL
BG257_RS19875 (BG257_19475) 72442..72807 - 366 WP_001052531 hypothetical protein -
BG257_RS20960 72819..72962 - 144 WP_001275801 hypothetical protein -
BG257_RS19880 (BG257_19480) 73143..73805 - 663 WP_001231463 hypothetical protein -
BG257_RS19885 (BG257_19485) 73805..74182 - 378 WP_000869261 hypothetical protein -
BG257_RS20965 74424..74600 - 177 WP_169342285 hypothetical protein -
BG257_RS19895 (BG257_19490) 74648..75277 - 630 WP_000743450 DUF4400 domain-containing protein tfc7
BG257_RS19900 (BG257_19495) 75234..75779 - 546 WP_000228720 hypothetical protein -
BG257_RS19905 (BG257_19500) 75829..77694 - 1866 WP_000178856 conjugative transfer system coupling protein TraD virb4
BG257_RS19910 (BG257_19505) 77691..80669 - 2979 WP_001337757 MobH family relaxase -
BG257_RS19915 (BG257_19510) 80837..81454 + 618 WP_001249396 hypothetical protein -
BG257_RS19920 (BG257_19515) 81436..81669 + 234 WP_001191892 hypothetical protein -

Host bacterium

ID   22027 GenBank   NZ_CP017084
Plasmid name   pT212 Incompatibility group   IncA/C2
Plasmid size   171489 bp Coordinate of oriT [Strand]   75628..75778 [-]
Host baterium   Proteus mirabilis strain T21

Cargo genes

Drug resistance gene   blaCTX-M-14, sul2, aph(3'')-Ib, aph(6)-Id, blaTEM-1B, aac(3)-IId, dfrA12, aadA2, aph(3')-Ia, rmtB, tet(G)
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -