Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   121550
Name   oriT_pTX1 in_silico
Organism   Thauera sp. K11
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP023440 (23231..23333 [+], 103 nt)
oriT length   103 nt
IRs (inverted repeats)      65..70, 73..78  (ACCGGC..GCCGGT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 103 nt

>oriT_pTX1
GCCTCGCAGAGCAGGATTCCGTTGAGCGCCCCCGGCGCGAATAAGGCCAAAGCGAAGGGGGATGACCGGCTTGCCGGTTAGCCAACTTCGCCCATCCTGCCCG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   13589 GenBank   WP_096452955
Name   traI_CCZ27_RS22570_pTX1 insolico UniProt ID   _
Length   777 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 777 a.a.        Molecular weight: 83692.30 Da        Isoelectric Point: 10.9342

>WP_096452955.1 TraI/MobA(P) family conjugative relaxase [Thauera sp. K11]
MIAKHVPMRTLARSNFAELVSYLTDAQDKTERLGDIRVTNCAAATLPAVIGEVLATQRQNTRAAGDKTFH
LLVSFRPGEAPEAATLQAIEARLAAGLGFAEHQRVSAVHHDTDNVHLHIAINKIHPERRTLHEPFQSYRT
LADLCTTLEREYGLQTDNHVSRRSVAEGRAADMERHAGVESLLGWVRRVCLEDLLGAHSWAQLHQVLSDN
GLVLRPRGNGLIVESRDGTRVKASTLARELSKPALEARLGPFEAPTEVLADGASDGPPSPSPAVGPHYSK
APVRLRIDTTALHVRYQAEQQRSSAQRSEALASARRRKLRAVDDARRANRLWRATIRVADGKGIDKRLLY
AQASRALRERLAQIHTAYARERAVLYDGFRRRTWADWLKHEALQGNAEALAALRAREAAQGLTGNTLAGA
GTGAGANVDPLGPGPDPVQDTVTKKGTILFRAGSSAVRDDGDRLQVSRTLTREGVKVALGLAMARYGGEI
TVCGTPEFKALVIRVAVDARFPLTFADPALEGRRQRLFTQETAHESTEHPERGRADRGRPRGAGARANRY
GSRSDERSGDRPDENRAGAGVDAHARAAGKPHPGRAGGVPPPASRHRLRGLSELGVVRFAGGGEVLLPGD
VPHHLEQRGAAPDHPLRRGVAGAGGLRAGRMTPAQRAAAGRYIAEREEKRQKGIDIPKHDLYDGRAGVIF
FAGLRTVDGQSLALLRPGDAASSVRASGNAGAERGADVVLVLPVDAATARRLSRVPRGDAVSVSPHGTIQ
RTGGHSR

  Protein domains


Predicted by InterproScan.

Large polyvalent protein-associated domain 7 (LPD7)

(438-526)

Relaxase/Mobilisation nuclease domain (Relaxase)

(14-251)


  Protein structure



No available structure.




T4CP

ID   15873 GenBank   WP_096452953
Name   t4cp2_CCZ27_RS22565_pTX1 insolico UniProt ID   _
Length   634 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 634 a.a.        Molecular weight: 68939.99 Da        Isoelectric Point: 8.9404

>WP_096452953.1 type IV secretory system conjugative DNA transfer family protein [Thauera sp. K11]
MKLEVNNAVGPQVRAGRAPGGKLLPVLGAVSLAGGLQSATQFFAHRFGYPAQLGEHLHHVYAPWAILEWA
SNWSARYPDELLQAASVGMMVSALGLFGVAVAKVVSSNSSRANATLHGSARWAGKTDIEAAGLLPRPRTL
RDVLTGEAARRSAGVYVGGWEDARGRFHYLRHDGPEHVLTYAPTRSGKGVGLVVPTLLSWGASAVVTDLK
GELWALTAGWRKAHARNTVLRFEPATASGSVCWNPLDEIRLETEDAVGDVQNLVTLIVDPNGKGLESHWQ
KTAFALLVGVILHALYKAKADGVSATLPAVDAMLADPNRDVGELWMEMATTGHLDGQPHPAIASAARDMM
DRPEEEAGSVLSTAKSYLALYRDPVVARNVSRSEFRIRDLMHADDPVTLYIVTQPNDKDRLRPLVRILVS
MVVRRLADRMAFAHGRPVAHYRHRLLLMLDEFAALGRLPILQEALAFVAGYGIKCYLICQDLNQLKSRET
GYGPDETITSNCHVQNAYPPNRLETAEHLSRLTGQTTVVKAQVTTSGRRTAAMLGQVSRTFQEVQRPLLT
PDECLRMPGPRKGADGHIEAPGDMVVYVAGFPAIYGKQPLYFKDPVFRARAAVPAPAVTDRLVSFADTGE
AIRL

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(121-620)

  Protein structure



No available structure.



ID   15874 GenBank   WP_096453085
Name   t4cp2_CCZ27_RS23030_pTX1 insolico UniProt ID   _
Length   855 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 855 a.a.        Molecular weight: 94134.97 Da        Isoelectric Point: 6.5500

>WP_096453085.1 MULTISPECIES: VirB4 family type IV secretion/conjugal transfer ATPase [unclassified Thauera]
MIAAIAFVVAGLGAALLLILVARIRAVEARVQLRRHRSRDAGLADLLNYAAVVDDGVIVGKNGAFMAAWC
YRGEDNASRTDEAREQVSVRLNQALAGLGSGWMVHVDAVRRPAPGYPERGRSHFPDAVSAAIDEERRRLF
ERLGTMYEGYFVLTLTWFPPVLAQRKFVELMFDDDAIAPDHTARTMNLIAQFRREIAAIESRLSAAVTLT
RLRGEPRVEEDGSTVTHDAFLGWLQCCLTGAHHPVRLPANPMYLDAVIGGQELWGGVVPRIGRQFIQVVA
IEGFPLESAPGLLTALGELPCAYRWSSRFIFMDPHEAVRHLERYRKKWRQKVRGFFDQVFNTHLGPIDQD
ALSMVADAEAAIAEVNSGLVAQGYYTSVVVLMDEDRDALEASARLIEKAINRLGFVARIETINTLDAFLG
SLPGHGVENVRRPLINTMNLADLLPTSSIWTGSPEAPCPLYPSGAPALMHGVTQGATPFRLNLHVRDLGH
TFMFGPTGAGKSAHLALIAAQLRRYAGMSIYAFDKGLSLYALTRAVGGQHYAVASDDGRLAFCPLQFLES
RGDRAWAMEWIETVLALNGVEATPAQRNEIGHAILSMQRSGARTLSEFSVTIQDEAIRQAIRQYTVDGAM
GHLLDAEEDGLALAGFTTFELEELLALGEKYALPVLLYLFRRIERSLSGQPAAILLDEAWVMLGHPVFRA
KIREWLKVLRKANCLVLMATQSLSDAANSGLLDVIVESTATKIFLPNVFARDPDTAALYRRMGLNTRQIE
ILASAVPKRQYYYVSESGHRLYELALGPIALAFVGATDKASVAAIQRLEARVGERWVHEWLAARGLTIND
QRDDGDPVDAIREAA

  Protein domains


Predicted by InterproScan.

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(221-431)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 112972..123503

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CCZ27_RS22995 (CCZ27_22995) 108083..109513 - 1431 WP_096453073 IS1182 family transposase -
CCZ27_RS23000 (CCZ27_23000) 109816..110145 + 330 WP_232516694 hypothetical protein -
CCZ27_RS23005 (CCZ27_23005) 110854..111882 - 1029 WP_096453152 ParB/RepB/Spo0J family partition protein -
CCZ27_RS23010 (CCZ27_23010) 112310..112672 + 363 WP_096453077 transcriptional regulator -
CCZ27_RS23015 (CCZ27_23015) 112972..113931 + 960 WP_096453079 P-type conjugative transfer ATPase TrbB virB11
CCZ27_RS23020 (CCZ27_23020) 113945..114391 + 447 WP_096453081 conjugal transfer system pilin TrbC virB2
CCZ27_RS23025 (CCZ27_23025) 114393..114704 + 312 WP_096453083 conjugal transfer protein TrbD virB3
CCZ27_RS23030 (CCZ27_23030) 114701..117268 + 2568 WP_096453085 VirB4 family type IV secretion/conjugal transfer ATPase virb4
CCZ27_RS23035 (CCZ27_23035) 117265..118065 + 801 WP_096453087 conjugal transfer protein TrbF virB8
CCZ27_RS23040 (CCZ27_23040) 118082..118978 + 897 WP_096453090 P-type conjugative transfer protein TrbG virB9
CCZ27_RS23045 (CCZ27_23045) 118981..119457 + 477 WP_096453092 conjugal transfer protein TrbH -
CCZ27_RS23050 (CCZ27_23050) 119463..120866 + 1404 WP_096453094 TrbI/VirB10 family protein virB10
CCZ27_RS23055 (CCZ27_23055) 120885..121646 + 762 WP_096453096 P-type conjugative transfer protein TrbJ virB5
CCZ27_RS23060 (CCZ27_23060) 121661..121891 + 231 WP_096453098 entry exclusion lipoprotein TrbK -
CCZ27_RS23065 (CCZ27_23065) 121902..123503 + 1602 WP_096453100 P-type conjugative transfer protein TrbL virB6
CCZ27_RS23070 (CCZ27_23070) 123615..124193 + 579 WP_096453102 TrbM/KikA/MpfK family conjugal transfer protein -
CCZ27_RS23075 (CCZ27_23075) 124196..124717 + 522 WP_096453104 transglycosylase SLT domain-containing protein -
CCZ27_RS23080 (CCZ27_23080) 125092..125817 - 726 WP_096453106 CBASS effector endonuclease NucC -
CCZ27_RS23085 (CCZ27_23085) 125818..126765 - 948 WP_096453108 AAA family ATPase -
CCZ27_RS23090 (CCZ27_23090) 126765..127280 - 516 WP_096453154 hypothetical protein -
CCZ27_RS23095 (CCZ27_23095) 127286..128251 - 966 WP_096453110 CBASS oligonucleotide cyclase -

Host bacterium

ID   21977 GenBank   NZ_CP023440
Plasmid name   pTX1 Incompatibility group   -
Plasmid size   140963 bp Coordinate of oriT [Strand]   23231..23333 [+]
Host baterium   Thauera sp. K11

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   czcA
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIB