Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   121549
Name   oriT_AR_0156|unitig_1 in_silico
Organism   Proteus mirabilis strain AR_0156
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP021853 (117276..117426 [+], 151 nt)
oriT length   151 nt
IRs (inverted repeats)      93..98, 106..111  (TGGCCT..AGGCCA)
 12..17, 24..29  (AACCCT..AGGGTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 151 nt

>oriT_AR_0156|unitig_1
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   13588 GenBank   WP_001326173
Name   mobH_AM403_RS20480_AR_0156|unitig_1 insolico UniProt ID   F5BPR1
Length   990 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 990 a.a.        Molecular weight: 109356.90 Da        Isoelectric Point: 4.9395

>WP_001326173.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGSTESSKPD
AGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDA
FSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQNSLQGDDFPMFGSSDEPPSWAIEPLPMLTDA
PEQTTPAPAMPPTDKPNLHEKDAKTLLVEMLAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILY
PDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDA
FELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ
PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDINAEDFLPSGVT
PQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKKRQ
GKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure


Source ID Structure
AlphaFold DB F5BPR1


T4CP

ID   15871 GenBank   WP_000178857
Name   traD_AM403_RS20485_AR_0156|unitig_1 insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69225.71 Da        Isoelectric Point: 6.9403

>WP_000178857.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPDDMWLGSGFLWENRHAQRVFEILKRDWTSIVGRESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVMPDWEAEAAAYLEKVKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-299)

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

  Protein structure



No available structure.



ID   15872 GenBank   WP_000637384
Name   traC_AM403_RS20560_AR_0156|unitig_1 insolico UniProt ID   _
Length   815 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 815 a.a.        Molecular weight: 92604.86 Da        Isoelectric Point: 5.9216

>WP_000637384.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIVTIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSAGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTTLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTDRIFAKTNKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQQLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSQGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRRQAA

  Protein domains


Predicted by InterproScan.

Family of unknown function (DUF5934) (DUF5934)

(284-434)

TraC protein (TraC_F_IV)

(21-255)

TraG P-loop domain (P-loop_TraG)

(449-809)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 115360..140503

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM403_RS20470 (AM403_20470) 111391..111624 - 234 WP_001191890 hypothetical protein -
AM403_RS20475 (AM403_20475) 111606..112223 - 618 WP_001249395 hypothetical protein -
AM403_RS20480 (AM403_20480) 112391..115363 + 2973 WP_001326173 MobH family relaxase -
AM403_RS20485 (AM403_20485) 115360..117225 + 1866 WP_000178857 conjugative transfer system coupling protein TraD virb4
AM403_RS20490 (AM403_20490) 117275..117820 + 546 WP_000228720 hypothetical protein -
AM403_RS20495 (AM403_20495) 117777..118406 + 630 WP_000743449 DUF4400 domain-containing protein tfc7
AM403_RS21535 118454..118630 + 177 WP_169342285 hypothetical protein -
AM403_RS20505 (AM403_20505) 118872..119249 + 378 WP_000869297 hypothetical protein -
AM403_RS20510 (AM403_20510) 119249..119911 + 663 WP_001231464 hypothetical protein -
AM403_RS21540 120092..120235 + 144 WP_001275801 hypothetical protein -
AM403_RS20515 (AM403_20515) 120247..120612 + 366 WP_001052530 hypothetical protein -
AM403_RS20520 (AM403_20520) 120757..121038 + 282 WP_000805625 type IV conjugative transfer system protein TraL traL
AM403_RS20525 (AM403_20525) 121035..121661 + 627 WP_001049717 TraE/TraK family type IV conjugative transfer system protein traE
AM403_RS20530 (AM403_20530) 121645..122562 + 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
AM403_RS20535 (AM403_20535) 122562..123875 + 1314 WP_024131605 TraB/VirB10 family protein traB
AM403_RS20540 (AM403_20540) 123872..124450 + 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
AM403_RS20545 (AM403_20545) 124454..124846 + 393 WP_000479535 TraA family conjugative transfer protein -
AM403_RS20550 (AM403_20550) 125047..130533 + 5487 WP_000606835 Ig-like domain-containing protein -
AM403_RS20555 (AM403_20555) 130682..131389 + 708 WP_001259346 DsbC family protein -
AM403_RS20560 (AM403_20560) 131386..133833 + 2448 WP_000637384 type IV secretion system protein TraC virb4
AM403_RS20565 (AM403_20565) 133848..134165 + 318 WP_000351984 hypothetical protein -
AM403_RS20570 (AM403_20570) 134162..134692 + 531 WP_001010740 S26 family signal peptidase -
AM403_RS20575 (AM403_20575) 134655..135920 + 1266 WP_001447719 TrbC family F-type conjugative pilus assembly protein traW
AM403_RS20580 (AM403_20580) 135917..136588 + 672 WP_000575345 EAL domain-containing protein -
AM403_RS20585 (AM403_20585) 136588..137592 + 1005 WP_005507569 TraU family protein traU
AM403_RS20590 (AM403_20590) 137708..140503 + 2796 WP_001256484 conjugal transfer mating pair stabilization protein TraN traN
AM403_RS20595 (AM403_20595) 140542..141402 - 861 WP_000709517 hypothetical protein -
AM403_RS20600 (AM403_20600) 141525..142166 - 642 WP_000796664 hypothetical protein -
AM403_RS20605 (AM403_20605) 142461..142781 + 321 WP_000547566 hypothetical protein -
AM403_RS20610 (AM403_20610) 143085..143270 + 186 WP_001186917 hypothetical protein -
AM403_RS20615 (AM403_20615) 143490..144458 + 969 WP_000085160 AAA family ATPase -
AM403_RS20620 (AM403_20620) 144469..145377 + 909 WP_000739139 hypothetical protein -

Host bacterium

ID   21976 GenBank   NZ_CP021853
Plasmid name   AR_0156|unitig_1 Incompatibility group   IncA/C2
Plasmid size   180262 bp Coordinate of oriT [Strand]   117276..117426 [+]
Host baterium   Proteus mirabilis strain AR_0156

Cargo genes

Drug resistance gene   qacE, sul1, blaKPC-2, tet(D), floR, sul2, dfrA14, ARR-3, cmlA1, blaOXA-10
Virulence gene   -
Metal resistance gene   merE, merD, merA, merP, merT, merR
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -