Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 121504 |
| Name | oriT_pWP5-S18-CRE-02_2  |
| Organism | Enterobacter cloacae strain WP5-S18-CRE-02 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_AP022128 (64825..64930 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_pWP5-S18-CRE-02_2
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 13559 | GenBank | WP_050868819 |
| Name | traI_H7R61_RS26760_pWP5-S18-CRE-02_2 |
UniProt ID | A0A142BMF1 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75234.98 Da Isoelectric Point: 10.0285
>WP_050868819.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPVYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSERDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPVYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSERDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A142BMF1 |
T4CP
| ID | 15845 | GenBank | WP_011091092 |
| Name | t4cp2_H7R61_RS26475_pWP5-S18-CRE-02_2 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79491.81 Da Isoelectric Point: 4.8270
>WP_011091092.1 MULTISPECIES: hypothetical protein [Gammaproteobacteria]
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFADWLDGTDTDDVLYHQEDDLFPMIEM
MQQESVDSKKVIRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMHFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFADWLDGTDTDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 67726..89274
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| H7R61_RS26740 (WP5S18C02_P20800) | 62810..63121 | + | 312 | WP_011091065 | hypothetical protein | - |
| H7R61_RS26745 (WP5S18C02_P20810) | 63254..63790 | + | 537 | WP_011091066 | hypothetical protein | - |
| H7R61_RS26750 (WP5S18C02_P20830) | 64291..64656 | - | 366 | WP_011091067 | hypothetical protein | - |
| H7R61_RS27600 | 64671..65249 | + | 579 | WP_223811519 | plasmid mobilization protein MobA | - |
| H7R61_RS26760 (WP5S18C02_P20850) | 65236..67215 | + | 1980 | WP_050868819 | TraI/MobA(P) family conjugative relaxase | - |
| H7R61_RS26765 (WP5S18C02_P20860) | 67229..67729 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| H7R61_RS26770 (WP5S18C02_P20870) | 67726..68505 | + | 780 | WP_004187315 | type IV secretory system conjugative DNA transfer family protein | traI |
| H7R61_RS26775 (WP5S18C02_P20880) | 68516..69679 | + | 1164 | WP_004187313 | plasmid transfer ATPase TraJ | virB11 |
| H7R61_RS26780 (WP5S18C02_P20890) | 69669..69929 | + | 261 | WP_004187310 | IcmT/TraK family protein | traK |
| H7R61_RS27605 (WP5S18C02_P20900) | 69954..73166 | + | 3213 | WP_011091070 | LPD7 domain-containing protein | - |
| H7R61_RS26790 (WP5S18C02_P20910) | 73132..73644 | + | 513 | WP_011091071 | hypothetical protein | traL |
| H7R61_RS26795 (WP5S18C02_P20920) | 73645..73857 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| H7R61_RS26800 (WP5S18C02_P20930) | 73829..74239 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| H7R61_RS26805 (WP5S18C02_P20940) | 74307..75020 | + | 714 | WP_109914255 | DotI/IcmL family type IV secretion protein | traM |
| H7R61_RS26810 (WP5S18C02_P20950) | 75029..76180 | + | 1152 | WP_011091074 | DotH/IcmK family type IV secretion protein | traN |
| H7R61_RS26815 (WP5S18C02_P20960) | 76192..77541 | + | 1350 | WP_011091075 | conjugal transfer protein TraO | traO |
| H7R61_RS26820 (WP5S18C02_P20970) | 77553..78257 | + | 705 | WP_011091076 | conjugal transfer protein TraP | traP |
| H7R61_RS26825 (WP5S18C02_P20980) | 78281..78811 | + | 531 | WP_011091077 | conjugal transfer protein TraQ | traQ |
| H7R61_RS26830 (WP5S18C02_P20990) | 78828..79217 | + | 390 | WP_011091078 | DUF6750 family protein | traR |
| H7R61_RS26835 (WP5S18C02_P21000) | 79263..79757 | + | 495 | WP_011091080 | hypothetical protein | - |
| H7R61_RS26840 (WP5S18C02_P21010) | 79754..82804 | + | 3051 | WP_011091081 | hypothetical protein | traU |
| H7R61_RS26845 (WP5S18C02_P21020) | 82801..84009 | + | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| H7R61_RS26850 (WP5S18C02_P21030) | 84006..84656 | + | 651 | WP_011091083 | hypothetical protein | - |
| H7R61_RS26855 (WP5S18C02_P21040) | 84649..86328 | + | 1680 | WP_232101164 | DotA/TraY family protein | traY |
| H7R61_RS26860 (WP5S18C02_P21050) | 86335..87870 | - | 1536 | WP_001000409 | IS66 family transposase | - |
| H7R61_RS26865 (WP5S18C02_P21060) | 87920..88267 | - | 348 | WP_000609174 | IS66 family insertion sequence element accessory protein TnpB | - |
| H7R61_RS26875 (WP5S18C02_P21070) | 88741..89274 | + | 534 | WP_232101165 | DotA/TraY family protein | traY |
| H7R61_RS26880 (WP5S18C02_P21080) | 89277..89930 | + | 654 | WP_011091084 | hypothetical protein | - |
| H7R61_RS26885 (WP5S18C02_P21090) | 90005..90235 | + | 231 | WP_011091085 | IncL/M type plasmid replication protein RepC | - |
| H7R61_RS26890 (WP5S18C02_P21100) | 90533..91588 | + | 1056 | WP_011091086 | plasmid replication initiator RepA | - |
Host bacterium
| ID | 21931 | GenBank | NZ_AP022128 |
| Plasmid name | pWP5-S18-CRE-02_2 | Incompatibility group | IncL/M |
| Plasmid size | 92491 bp | Coordinate of oriT [Strand] | 64825..64930 [+] |
| Host baterium | Enterobacter cloacae strain WP5-S18-CRE-02 |
Cargo genes
| Drug resistance gene | blaTEM-1B, dfrA12, aadA2, qacE, sul1, armA, msr(E), mph(E), blaCTX-M-3 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |