Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   121503
Name   oriT_FDAARGOS_734|unnamed1 in_silico
Organism   Burkholderia cenocepacia strain FDAARGOS_734
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP054818 (29842..30542 [-], 701 nt)
oriT length   701 nt
IRs (inverted repeats)      597..602, 607..612  (GCGGGG..CCCCGC)
 535..540, 545..550  (CGCGCC..GGCGCG)
 175..183, 197..205  (TGTCAGACA..TGTCTGACA)
 37..45, 51..59  (GAGTCGAGG..CCTCGACTC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 701 nt

>oriT_FDAARGOS_734|unnamed1
GAAAAACGCCTGGAATGAAAAGAGCGGGCGCTCGTGGAGTCGAGGAAAAACCTCGACTCGCGCACGAAACCGCGCTCGATTACGGCCCTCATGACTGCATTATTTTGAGCGACAGTAGCATGGCGACGAGCGTTTCGCAATAACCCATTTATTTTCATAGTGATACTTATCGAATGTCAGACACACCCGTTCTAGCTGTCTGACAACGTGGCCCAATCATGACGGGGCGGGCGCGACATACTGGCAATGTTCGCGCTTTCCGGTCATTCGGGTGTCTGACATCGCGAGCCTTAACGCAAGCCGTTTCTCGCGATACGTGGCGGCGTTTTCTCGACAGGTCGACGGTGGCTGTCTGACGCTGCAAACCGCTACCGTCAGTGGTCGAGCGGGAATTTTCTATTCCTCTGGAGTCCGAATTATATTCTCCGATTCCGAATTTGTCTGACACCGGAGGATATGACGTACAAGGGGTCGCGCTTGATCCCTGCCTCGCTTTTTTCGCCTATTTTCGTAGGTGTCTGACGCCGAAGGCACCGCGCCATACGGCGCGACACACCGCAACAACGTTGCATGGTGTGTGACGATCGTCTTAGGGGGCGGGGATTCCCCCGCTTCCTAAGACGATCGTAGCCAACTTCACTTCAGGTTTGGGCCGGTCGCGGAGCCTTCACCCGGTAAGGTACGGCGGTTCCCGCTCAAGG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   13558 GenBank   WP_006488612
Name   mobF_FOC42_RS14650_FDAARGOS_734|unnamed1 insolico UniProt ID   B4EQJ6
Length   939 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 939 a.a.        Molecular weight: 104810.59 Da        Isoelectric Point: 6.4316

>WP_006488612.1 MobF family relaxase [Burkholderia cenocepacia]
MLSLHNIASAAQTRNYFSKDNYYTQDENTKHSAWFGAGAARLGLAGQVQPAAFLNLLEGRVEEQQLGKWV
TDEETGERRQDHQPGIDITFSAPKSVSLMAEVAGDTAVRDAHEAAVQSALAYLEENVIRTRSTQNGVTSI
EHTGNMVAALFKHNTSRDLDPQTHTHAVVMNATLRADGKWRSIDNREIYQTQKLAGAIYNAELVSGLQQL
GYRLTNPDRHGNFEIQGISREQIEHFSQRRTAMKEKLGKRQLELDTASAADKERAALATRERKVDVDHEQ
LLADWQARALEQGIDLGALRATAAERKAEGIDPPTKLTGRQALAFAAAHLIEREAVVDKSDLVETAVVHG
TGRVSPQEVMKAFRQLEKNGDLVPLPDEKYTTRKMLESERWALDFVKQEKGNAPVVLEATKVQERIAEAE
RLQQFSYTFGQRDAIVLALTSKDRFVAIQGLAGTGKTTMLRTLNDIAAAEGYVVRGMAPTGAAAKVMVKE
TGIAAETVAMFQIKERQLQKDIEFAQQFAPDFKRKPEVWVVDESSFLAQRQMAQLHRLAEHAGAKVVYLG
DTLQLQGVEAGKPFELAQKDGIATAYMTDINRQKTDVMKEAVDIITGRNQLDAGTRLTDIELKRNGQSFQ
YLSQQGRVHETDEVVTSTVDKILAMSPEERARNITITPLNRDRVAINEGVRVGLQARGELSKNESTHEIL
VSKGFTRAQIKEAQYYERGDVVRFNRTYQQINADKGDYTRVIDVDHESGIVTLRKRDGSTLAWEPAKHNK
VEVYDPDLRQLAVGDVIRLTRNDETFKNGETARVVQLTGEHAVLQVDDRGVISHHSVNLETSQHWDHAYA
STVHAAQGASRNTTIFAINMPDNEDKKAQEREFQALAKVFGDRSYYVGVTRASHDISIVTNDIDLAQRAI
TGKLDKTTVIEAMNQEVREQTIQREQHEI

  Protein domains


Predicted by InterproScan.

AAA domain (AAA_30)

(427-609)

TrwC relaxase (TrwC)

(12-290)


  Protein structure


Source ID Structure
AlphaFold DB B4EQJ6


T4CP

ID   15844 GenBank   WP_006488610
Name   t4cp2_FOC42_RS14640_FDAARGOS_734|unnamed1 insolico UniProt ID   _
Length   639 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 639 a.a.        Molecular weight: 72007.72 Da        Isoelectric Point: 6.9695

>WP_006488610.1 type IV secretion system DNA-binding domain-containing protein [Burkholderia cenocepacia]
MTGKKPNGLFQDFSTGSEIWTHRVALVLVAIRNILLLSLFLGLAAGFIYAAFFVDRDIWRPAVANVIAHL
RSVVMMTAVPMEYEVNGQTVRLPVDQVVALTDPVWDVAYFFLRRFLIIVATVTACTCVSIAAYWYEFGRS
TMADKDLRGATLVNGKQLAKMIRQDDMESPYSIAGVPMLRNAETLHTLVVGAQGTGKSQQFFDRLRQVRA
RGKRAIVYDPSGEFAEEFFREGKDVMMNPFDDRSPNWNIKYEIEEEYHYDSMATALIPDPREADPFWSLA
GREVFRDVARTLHREGNLTNRNLYNSIAMSNLDAIYQLLRNTAGASYVDPKTERTGMSLKMTVQNQLSSF
RYLRDDGKPFSIRQWIYNESDSWMFISTREEMLEAIKPIMSLWIDIAIKAVLNLTPIHRERLHFSIDEVP
TLQKLDIMKLAVSNTRKYGLCMMLGAQDMPQFLDIYGEYLARTIINGCQTKLLLRVTDAEAAEMFSKIIG
QTEVEEKDESLSFGVNSQRDGFSIAKRRLLRDLALPSQILRLRDMTGYLVYPGEYPIARVKYGFVKPRKN
APAFIKRQAFSELFAPAAGSPATAAASESVVAGPSPDWIAHPEILVAASGGVDPETGEILPPRPGAWRWG
DGNQRGDHA

  Protein domains


Predicted by InterproScan.

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(171-552)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 43394..69875

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOC42_RS14660 (FOC42_14655) 39552..40412 + 861 WP_227743146 LuxR family transcriptional regulator -
FOC42_RS14665 (FOC42_14660) 40535..41575 + 1041 WP_006493948 TRAP transporter substrate-binding protein DctP -
FOC42_RS14670 (FOC42_14665) 41572..41808 + 237 WP_006487429 hypothetical protein -
FOC42_RS14675 (FOC42_14670) 41795..42565 + 771 WP_006487404 5-oxoprolinase subunit PxpA -
FOC42_RS14680 (FOC42_14675) 42687..42959 + 273 WP_006493934 hypothetical protein -
FOC42_RS14685 (FOC42_14680) 43394..43870 + 477 WP_006487418 lytic transglycosylase domain-containing protein virB1
FOC42_RS14690 (FOC42_14685) 43867..44211 + 345 WP_006493942 hypothetical protein -
FOC42_RS14695 (FOC42_14690) 44240..44632 + 393 WP_006487426 hypothetical protein -
FOC42_RS14700 (FOC42_14695) 44646..44939 + 294 WP_006487365 type IV conjugative transfer system protein TraL traL
FOC42_RS14705 (FOC42_14700) 44946..45536 + 591 WP_006487381 TraE/TraK family type IV conjugative transfer system protein traE
FOC42_RS14710 (FOC42_14705) 45475..46221 + 747 WP_012493842 type-F conjugative transfer system secretin TraK traK
FOC42_RS14715 (FOC42_14710) 46214..47647 + 1434 WP_006487384 TraB/VirB10 family protein traB
FOC42_RS14720 (FOC42_14715) 47640..48434 + 795 WP_006487398 DsbC family protein trbB
FOC42_RS14725 (FOC42_14720) 48431..49093 + 663 WP_006487356 type IV conjugative transfer system lipoprotein TraV traV
FOC42_RS14730 (FOC42_14725) 49096..51702 + 2607 WP_006487362 type IV secretion system protein TraC virb4
FOC42_RS14735 (FOC42_14730) 51699..52214 + 516 WP_006487408 hypothetical protein -
FOC42_RS14740 (FOC42_14735) 52211..52951 + 741 WP_006487402 hypothetical protein -
FOC42_RS14745 (FOC42_14740) 52948..53562 + 615 WP_006487383 hypothetical protein -
FOC42_RS14750 (FOC42_14745) 53559..53951 + 393 WP_006493936 hypothetical protein -
FOC42_RS14755 (FOC42_14750) 53948..54313 + 366 WP_006487376 hypothetical protein -
FOC42_RS14760 (FOC42_14755) 54389..54922 + 534 WP_020980048 phospholipase D family protein -
FOC42_RS14765 (FOC42_14760) 54936..55748 + 813 WP_006487420 FadR/GntR family transcriptional regulator -
FOC42_RS14770 (FOC42_14765) 55726..55944 + 219 WP_006487354 hypothetical protein -
FOC42_RS14775 (FOC42_14770) 55962..56492 + 531 WP_006487449 signal peptidase I -
FOC42_RS14780 (FOC42_14775) 56479..57117 + 639 WP_006487379 type-F conjugative transfer system protein TraW traW
FOC42_RS14785 (FOC42_14780) 57114..58124 + 1011 WP_006487375 conjugal transfer pilus assembly protein TraU traU
FOC42_RS14790 (FOC42_14785) 58121..58822 + 702 WP_006487447 type-F conjugative transfer system pilin assembly protein TrbC trbC
FOC42_RS14795 (FOC42_14790) 58824..59201 + 378 WP_006493944 hypothetical protein -
FOC42_RS14800 (FOC42_14795) 59203..60324 + 1122 WP_006487394 hypothetical protein -
FOC42_RS14805 (FOC42_14800) 60332..61576 + 1245 WP_006487422 conjugal transfer protein TraN traN
FOC42_RS14810 (FOC42_14805) 61573..61839 + 267 WP_174900806 hypothetical protein -
FOC42_RS14815 (FOC42_14810) 61836..62144 + 309 WP_006493941 hypothetical protein -
FOC42_RS14820 (FOC42_14815) 62141..62530 + 390 WP_006493947 hypothetical protein -
FOC42_RS14825 (FOC42_14820) 62535..63368 + 834 WP_006487410 conjugal transfer protein TraF traF
FOC42_RS14830 (FOC42_14825) 63379..63909 + 531 WP_006487388 hypothetical protein -
FOC42_RS14835 (FOC42_14830) 63906..64349 + 444 WP_006487377 HNH endonuclease -
FOC42_RS14840 (FOC42_14835) 64346..65380 + 1035 WP_006487369 phage Gp37/Gp68 family protein -
FOC42_RS14845 (FOC42_14840) 65373..66827 + 1455 WP_006487442 conjugal transfer protein TraH traH
FOC42_RS14850 (FOC42_14845) 66837..69875 + 3039 WP_006487373 conjugal transfer protein TraG N-terminal domain-containing protein traG
FOC42_RS14855 (FOC42_14850) 69865..70245 - 381 WP_020980049 hypothetical protein -
FOC42_RS14860 (FOC42_14855) 70889..71155 + 267 WP_006487406 hypothetical protein -
FOC42_RS14865 (FOC42_14860) 71166..72578 + 1413 WP_006487358 zeta toxin family protein -
FOC42_RS14870 (FOC42_14865) 72575..73891 + 1317 WP_006487416 DUF1173 domain-containing protein -
FOC42_RS14875 (FOC42_14870) 73888..74055 + 168 WP_006487400 hypothetical protein -
FOC42_RS14880 (FOC42_14875) 74052..74624 + 573 WP_006487431 PH domain-containing protein -

Host bacterium

ID   21930 GenBank   NZ_CP054818
Plasmid name   FDAARGOS_734|unnamed1 Incompatibility group   -
Plasmid size   92661 bp Coordinate of oriT [Strand]   29842..30542 [-]
Host baterium   Burkholderia cenocepacia strain FDAARGOS_734

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -