Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 121502
• Name: oriT_pWP4-W18-CRE-03_1
• Organism: Pseudomonas putida strain WP4-W18-CRE-03
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_AP022056 (1752..1785 [-], 34 nt)
• oriT length: 34 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 34 nt

>oriT_pWP4-W18-CRE-03_1
ACGATTTTGCGAAGCAAAGTCTAGTGAGTACACT

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_H7R82_RS25245_pWP4-W18-CRE-03_1

• ID: 13557
• GenBank: WP_182943414
• Name: mobV_H7R82_RS25245_pWP4-W18-CRE-03_1
• UniProt ID: _
• Length: 323 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 323 a.a.
• Molecular weight: 35791.75 Da
• Isoelectric Point: 10.0313

>WP_182943414.1 MobV family relaxase [Pseudomonas putida]
MAYAIMRAKKLATMGSVAASMQHCYRERETHNADQERTPDNQHLVAKSTDEAMGKLRALLPEKRRKDAVL
AVEYVMTASPEWFATATPEQEKAFFQRSVQWLADKYGADRIITASIHRDEVTPHLSAFVVPLTQDKRLSA
KEFIGSRDKMRADQSTYAACVADLGLERGIEGSKATHQRIQQHYTAVQQGVESKAILSPQSIEPRVLEKA
GLLDKLRGHGDLVESPEMIAERLTKAVNKGFAGTVAKASESAQNARKAKEAQDTANTLRKRLEAIEGPFK
GLTKDQVREVIRVAMTFQQENAKAKEQSKSKVKTTGKDSGLSL

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-184)

Protein structure

No available structure.

Host bacterium

• ID: 21929
• GenBank: NZ_AP022056
• Plasmid name: pWP4-W18-CRE-03_1
• Incompatibility group: -
• Plasmid size: 2292 bp
• Coordinate of oriT [Strand]: 1752..1785 [-]
• Host baterium: Pseudomonas putida strain WP4-W18-CRE-03

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -