Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 121497
• Name: oriT_pWP2-W18-CRE-05_5
• Organism: Aeromonas sp. WP2-W18-CRE-05
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_AP021922 (11423..11471 [-], 49 nt)
• oriT length: 49 nt
• IRs (inverted repeats): 21..27, 31..37 (ACATGGC..GCCATGT)
• Location of nic site: 9..10
• Conserved sequence flanking the nic site: ATGCGGATTG
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 49 nt

>oriT_pWP2-W18-CRE-05_5
CAGGATGCGGATTGTCATACACATGGCTACGCCATGTTCCTGCCAATCC

oriT secondary structure

Predicted by RNAfold.

Relaxase

traI_H7R73_RS23865_pWP2-W18-CRE-05_5

• ID: 13553
• GenBank: WP_182951802
• Name: traI_H7R73_RS23865_pWP2-W18-CRE-05_5
• UniProt ID: _
• Length: 887 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 887 a.a.
• Molecular weight: 101665.14 Da
• Isoelectric Point: 9.8038

>WP_182951802.1 TraI/MobA(P) family conjugative relaxase [Aeromonas sp. WP2-W18-CRE-05]
MIVKKVPNSKTKHKTASIRDLADYIREPHNQNPDEKVLYANGRGFLCDGHAAQREEMVALAAESVRSKNP
VNHYIMSWREGEQPSPEQVEEAVSIFMEELGWKDHQAIYGLHKDTDNIHLHIAINRVHPETLKIVEINRG
FDIEMAHRAIARIEHAQGWQREQNGRYQVLENGELGRAPYDPEKPRQPDQRKRDMENRTGEKSALRIAIE
DGAAIIKRAQSWEQLHRELSAKGMRYEKTGSGATVFVGDVGVKASDVDRNASLAKMQKRLGEYQPAPQHQ
QVAQREPEPIKPDVPGWKDYIAGRKAHYAAKNAAKLALDKQQEQERKQLAEQQKARRDELMRGNWKGKGE
VLNAMRSVIAAEQAAEKAALKEKHQKQREQWRQQFRPYPDLEQWQRMQKSPELAEQWRHRASEPQRIEGD
RSEPPTPRDIRAYVPEIVGQQVHYSRKDDAERGGGVSFVDKGKSIDIHDWRNRDSTLAALQLSAQKWGSF
TVTGNDEYKAMCAKLAAEHGFKITNPELQESIQQERQRIQQERAQAMKSEQLKQFERYAEAVGAERYRVT
SIKMQADGRKQTFILDKKDGITRGFTPQEIAQRTPEMQRLQRRGENLYYTPLSDRKHHILIDDMNREKLE
RLIRDGYQPAVVLESSPGNYQAIITVSKLGTVHDKDVGNRLSDALNREYGDPKLSGAIHPHRAPGYENRK
PKHQREDGSYPEVRLLKAERRECVKALALSSQIDAEYQRQAALKAQQPERSKAKPALELAAASGSAIDAY
QRHYRDVLKRQRGGEVDLSRVDSMIAVRMRVTGHDQAAIEGAIRQCAPATRQKDEGRDWNDYAQRTARYA
YSAAGDRQAAELGKYRQQWEKLEGREPQRQQEQAKARKIERDSGLSL

Protein domains

Predicted by InterproScan.

• Large polyvalent protein-associated domain 7 (LPD7) (442-526)

• RepB DNA-primase from phage plasmid (RepB_primase) (547-806)

• Relaxase/Mobilisation nuclease domain (Relaxase) (15-273)

Protein structure

No available structure.

Host bacterium

• ID: 21924
• GenBank: NZ_AP021922
• Plasmid name: pWP2-W18-CRE-05_5
• Incompatibility group: -
• Plasmid size: 11490 bp
• Coordinate of oriT [Strand]: 11423..11471 [-]
• Host baterium: Aeromonas sp. WP2-W18-CRE-05

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -