Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 121479
• Name: oriT_SGT-39|5
• Organism: Aquicella lusitana strain SGT-39
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_LR699118 (17054..17084 [+], 31 nt)
• oriT length: 31 nt
• IRs (inverted repeats): 1..7, 11..17 (ACGTTGC..GCAACGT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 31 nt

>oriT_SGT-39|5
ACGTTGCGAAGCAACGTATAAGCGCGCACTT

oriT secondary structure

Predicted by RNAfold.

Relaxase

traA_AQUSIP_RS12275_SGT-39|5

• ID: 13540
• GenBank: WP_114835393
• Name: traA_AQUSIP_RS12275_SGT-39|5
• UniProt ID: _
• Length: 892 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 892 a.a.
• Molecular weight: 101847.72 Da
• Isoelectric Point: 7.3292

>WP_114835393.1 Ti-type conjugative transfer relaxase TraA [Aquicella lusitana]
MAIYHFSGTVISRSQGRSAVACAAYRSAEKLHDEKYDREHDYTHKQDVSSTEILLPENAPAWMADREKLW
NAVESHEKRKDAQLAREFNFALPRELTLEQNIALAKDFVKEAFVRKGMVADFCIHNDKQHDGQFHPHAHV
MLTMREVTQDGFGQKVRAWNDKAMLLNWREEWAEVANKHLALHGHDLRIDHRTLGEQGIALEPQHKIGAT
VARDRLVRMEDHQRIARENGDKLLADPSVALNAITRQQSTFTHQDIARFVHRHTVDAEQFSLVYEKVKSH
ESLVRLGIDEKDRERFTTNEMLSLESKMMERAVNLSKSDSHVVSDTHQEKALSQKSLTVEQKTAFDHLVG
GGDLKCVVGFAGTGKSYLLGAAKDAWEKEGYAVHGVTLSGIAAENLEGSSGIDSRTFASRCYYWDKGEQL
LTKKDVLVVDEAGMLSSRQMARLMEEASRGGAKVVLVGDPQQLQAIEAGAAFRAIAEQVHYVELTDIRRQ
SEKWQQDATKELATGKTTDAINRYDQHFHVHDFETKAVAKVALTEVWNDARLSNTDKTQIMLAYNRDDVL
ELNQIARGLRHKQGELGKDVLFQTERGERAFAERDRVYFLKNDKSLGVMNGTLGTIEAIKDGAITVRLDG
DGRTNVDRRVTVTMERYNQLDYGYAATIHKAQGVTVDRSYVLASKYLDAHSTYVAMSRHRESADLFYGRD
VFLNKDDLVKTLSRERTKDVTLDYLNSRDRRQEPVTRVMDEIKPLTRDEERLMRQAKLKEFTEATKAKRG
IDLDRSVDSLSGTKQRPDLSEFKRQFEAKNPERASALRSEVMPEHEKKALSLVNEFKRLEAIVDKGGRTS
RMAQDHLEKLADKISRQKDVMQYVREHHAPMEKQIDKQAREFQRDRGLELER

Protein domains

Predicted by InterproScan.

• MobA/MobL family (MobA_MobL) (17-212)

• AAA domain (AAA_30) (337-524)

Protein structure

No available structure.

T4CP

t4cp2_AQUSIP_RS12285_SGT-39|5

• ID: 15836
• GenBank: WP_170131887
• Name: t4cp2_AQUSIP_RS12285_SGT-39|5
• UniProt ID: _
• Length: 562 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 562 a.a.
• Molecular weight: 63477.53 Da
• Isoelectric Point: 7.6007

>WP_170131887.1 type IV secretory system conjugative DNA transfer family protein [Aquicella lusitana]
MKHQRYPKAENIFFSILIFIVSFVFGCIFFCLILGWIGLFFGLTLSISLVCLYLYWPCFIIWKIKRDTGK
KPSEKILEQFDERYDWLKKKIIEKGLVEIDKFEEINDKQIVIKTQITTGFSLWLGKSTGLLSTLWHRAGM
AANQQVALTLEDACQNVLVLGGIGSGKTTCVMQPLLLQCLDQHCGGLIFDIKGDVKEAVSQFAAATNRNL
VILGPHHRRMNLIEGLTPEVAASFLKSAFLLGNKGNVDSFWVDTASELCRNTLGMLSFLPKRYDLQSLHQ
YLFELDSQETINNEIDALLPILPEKKARLLKTYCNYHDLIFSHFDAKIKSGVNATIAQALAPFNHPDLLD
AFCSSHSEPAKMEDILNGCVYLVDMPLSIWGLGGKVAYTFIKLRFFNLMQNRNQHPTQNKNNPVFFMCDE
YQEIVSANRDGLSDLNFWDKSRSSKTIGIISSQSIASFYAALGSHDLAHALLQNFRQKLCLRTEDPVTLD
FMERLVGRARVKKVSDSLGNQNHTKTITESREGVIDAQLFRELHPGQAVTILSLSGHSMDDIVNLKPVYL
NN

Protein domains

Predicted by InterproScan.

• Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind) (146-266)

• Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind) (407-520)

Protein structure

No available structure.

Host bacterium

• ID: 21906
• GenBank: NZ_LR699118
• Plasmid name: SGT-39|5
• Incompatibility group: -
• Plasmid size: 20426 bp
• Coordinate of oriT [Strand]: 17054..17084 [+]
• Host baterium: Aquicella lusitana strain SGT-39

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -