Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 121477
• Name: oriT_1|2
• Organism: Pseudomonas aeruginosa isolate
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_LS998784 (6136..6272 [-], 137 nt)
• oriT length: 137 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 137 nt

>oriT_1|2
CGAATGGTCTGGCGTCCGTTGGAACCACGTGGCGAAGCCATGTGTGTTACAACGGCCATCCTGTTAAGCTCATCTGCTCTACTATCGTATCAGCAGTTTAGGTGCACGGGACGTGCTTTGCAACAAGCATAAAAGGC

oriT secondary structure

Predicted by RNAfold.

Relaxase

traI_PA5486_RS35130_1|2

• ID: 13538
• GenBank: WP_134163281
• Name: traI_PA5486_RS35130_1|2
• UniProt ID: _
• Length: 889 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 889 a.a.
• Molecular weight: 101309.85 Da
• Isoelectric Point: 9.8369

>WP_134163281.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Gammaproteobacteria]
MIVKKVPNSKTKHKTGSIRDLADYIREPHNQNPDEKVLYANGRGFLCDGHAAQREEMVALAAESVRSKNP
VNHYIMSWREGEQPSPEQVEEAVSIFMDELGVKDHQAIYGLHADTDNLHLHLAINRVHPETLKVVKINNG
FDIEAAHKAIARIENAQGWQREQNGRYQVLENGELGREHIDKDKPRQPAQPKRDMENRTGEKSAERIAIE
DGAPIIKKAQTWEQLHRELAAKGMRYEKTGSGATLFVGDVGVKASSADRDASLSKLQKRLGAYQPPPQRQ
QVAQREPEPIKPDVPGWKDYITGRKAHYAAKNAAKLALDKQQEQERKQLAEQQKARRDELMRGNWNGKGE
VLNAMRSVIAAEQAAEKAALKEKHKKQREQWRQQFRPYPDLEQWQRMQKSPELAEQWRHRASEPQRIEGA
SGEPPTPRDIRAYQPEIVGQQVHYSRKEEAGAGGGVSFVDKGKSIDIHDWRNRDSTLAALQLSAQKWGSF
TVTGNDEYKAMCAKLAAEHGFKITNPELQERIQQERQRIQQERAQAMKSEQLKQFELYAEAVGAERYRVT
SIKMQADGRKQTFILDKKDGITRGFTPQEIEQRTPEMLRLQRRGENLYYTPLSDKKHHILIDDMNREKLE
RLIRDGYRPAVVLESSPGNYQAIITVPKLGTAHDKDVGNRLSDALNREYGDPKLSGAIHPHRAPGYENRK
PKHQREDGSYPEVRLLKVERRECVKALALSSQIDAEYQRQAALKAQQPERSKAKPALELAAASGSAIDAY
QRHYRDVIKRQRGGEVDLSRVDSMIAVRMRVTGHDQAAIEGAIRQCAPATRQKDEGRDWNDYAQRTARYA
YSAAGDRQAAELGKYRQQWEKLEGREPVRQQEQAKAQKIERDNSPGMSR

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (17-272)

• Large polyvalent protein-associated domain 7 (LPD7) (438-526)

• RepB DNA-primase from phage plasmid (RepB_primase) (548-806)

Protein structure

No available structure.

Host bacterium

• ID: 21904
• GenBank: NZ_LS998784
• Plasmid name: 1|2
• Incompatibility group: -
• Plasmid size: 24853 bp
• Coordinate of oriT [Strand]: 6136..6272 [-]
• Host baterium: Pseudomonas aeruginosa isolate

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -