Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 121453
• Name: oriT_pGSH8-2
• Organism: Aeromonas hydrophila strain GSH8-2
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_AP019194 (13497..13634 [-], 138 nt)
• oriT length: 138 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 138 nt

>oriT_pGSH8-2
CGAATGGGCAGGCGTCCGTTGGAACCACGTGGCGAAGCCATGTGTGTTACAACGGTCATCCTGTATTGCTCATCCGCTCTACTATCGTATCAGCAGTTTAGGTGCACGGGACGCACTTTGCAACAAGCACAAATGGCG

oriT secondary structure

Predicted by RNAfold.

Relaxase

traI_EZU31_RS23100_pGSH8-2

• ID: 13526
• GenBank: WP_033996285
• Name: traI_EZU31_RS23100_pGSH8-2
• UniProt ID: A0A0U2DHM7
• Length: 890 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 890 a.a.
• Molecular weight: 101454.99 Da
• Isoelectric Point: 9.8485

>WP_033996285.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Pseudomonadota]
MIVKKVKNPQKAASKAVRVSRLTDYIREPERENSQEKCIHAGARGFITDEPQSQTAEMIALSQEAVRSKD
TINHYVLSWREGEQPSPEQVEEAVSIFMDELGVKDHQAIYGLHADTDNLHLHLAINRVHPETLKVVKINN
GFDIEAAHKAIARIENAQGWQREQNGRYQVLENGELGREHIDKDKPRQPAQPKRDMENRTGEKSAERIAI
EDGAPIIKKAQTWEQLHRELAAKGMRYEKTGSGATLFVGDVGVKASSADRDASLSKLQKRLGAYQPPPQR
QQVAQREPEPIKPDVPGWKDYITGRKAHYAEKNAAKLALDKRQEQERKQIAEQQKARRDELMRGNWKGKG
EVLNAMRSVIAAEQAAEKAALKEKHQKQREQHRQQFRPYPDLEQWQRMQKSPELAEQWRHRASEPQRIEG
ASGEPPTPRDIRAYQPEIVGQQVHYSRKEEAGAGGGVSFVDKGKSIDIHDWRNRDSTLAALQLSAQKWGS
FTVTGNDEYKAMCAKLAAEHGFKITNPELQERIQQERQRIQQERAQAMKSEQLKQFELYAEAVGAERYRV
TSIKMQADGRKQTFILDKKDGITRGFTPQEIEQRTPEMLRLQRRGENLYYTPLSDKKHHILIDDMNREKL
ERLIRDGYRPAVVLESSPGNYQAIITVPKLGTAHDKDVGNRLSDALNREYGDPKLSGAIHPHRAPGYENR
KPKHQREDGSYPEVRLLKAERRECVKALALSSQIDAEYQRQAALKAQQPERSKAKPALELAAASGSAIDA
YQRHYRDVIKRQRGGEVDLSRVDSMIAVRMRVTGHDQAAIEGAIRQCAPATRQKDEGRDWNDYAQRTARY
AYSAAGDRQAAELGKYRQQWEKLEGREPVRQQEQAKAQKIERDNSPGMSR

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (19-273)

• Large polyvalent protein-associated domain 7 (LPD7) (439-527)

• RepB DNA-primase from phage plasmid (RepB_primase) (549-807)

Protein structure

• AlphaFold DB: A0A0U2DHM7

Host bacterium

• ID: 21880
• GenBank: NZ_AP019194
• Plasmid name: pGSH8-2
• Incompatibility group: IncP6
• Plasmid size: 39071 bp
• Coordinate of oriT [Strand]: 13497..13634 [-]
• Host baterium: Aeromonas hydrophila strain GSH8-2

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -