Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 121452 |
| Name | oriT_E7471|4  |
| Organism | Enterococcus faecium isolate E7471 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_LR135334 (63654..63696 [+], 43 nt) |
| oriT length | 43 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 43 nt
>oriT_E7471|4
AATATCGCAACATGGGCCCATGTTGCTCTGCTCGCAAAAGAAA
AATATCGCAACATGGGCCCATGTTGCTCTGCTCGCAAAAGAAA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 13525 | GenBank | WP_127843788 |
| Name | Relaxase_EQB74_RS16690_E7471|4 |
UniProt ID | _ |
| Length | 555 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 555 a.a. Molecular weight: 63725.09 Da Isoelectric Point: 6.4729
>WP_127843788.1 relaxase/mobilization nuclease domain-containing protein [Enterococcus faecium]
MVYTKHFTIHSSKHLKQAEEYVENAAKTAIDSKRGIDSHLDNIFPYVTSDKKTINKQLVSGYGIIDVYNS
AKEFLLTKQSAAFAKGKDLVFNPKTGKVVLDPQALEKNNAVLAHHIIQSFSPDDDLTPEQIHEIGRKTIL
EFTGGEYEFVIATHIDKAHIHNHIILNSTNTFTGNALRWQKGTKKSFEQVSDKIASKYGAKIIEKSPKNS
HKKYTMWQTENLFKNKIKSRLDFLLDHSSSIDDFKIKAEALDLQVDFSGKWAKYCLLDEPQIKNTRSRSL
SKKDPEKYNLDRIEARLKENSHEFSVQEVLERYEEKVDAVNNDFDYQLILESWQISHKTPKGYYVNVDFG
AENHGQIFIGAYKVDQLEDGNYSLFVKKNDYFYFMNDKSSNRNRYMTGYTLAKQLSLYNGTVPLKKEPVM
TTITELVDAINFLAEHGVTESKQMTRLENKLIESFELAEESLQKLDDKIIQLNQLGKLILGEKLEDIDQE
FPVLKEVGDKDELTFEDIQNELSSAKLSRKLLHEKMESTVEKINELHAIQTVAEQKSSEENLGKL
MVYTKHFTIHSSKHLKQAEEYVENAAKTAIDSKRGIDSHLDNIFPYVTSDKKTINKQLVSGYGIIDVYNS
AKEFLLTKQSAAFAKGKDLVFNPKTGKVVLDPQALEKNNAVLAHHIIQSFSPDDDLTPEQIHEIGRKTIL
EFTGGEYEFVIATHIDKAHIHNHIILNSTNTFTGNALRWQKGTKKSFEQVSDKIASKYGAKIIEKSPKNS
HKKYTMWQTENLFKNKIKSRLDFLLDHSSSIDDFKIKAEALDLQVDFSGKWAKYCLLDEPQIKNTRSRSL
SKKDPEKYNLDRIEARLKENSHEFSVQEVLERYEEKVDAVNNDFDYQLILESWQISHKTPKGYYVNVDFG
AENHGQIFIGAYKVDQLEDGNYSLFVKKNDYFYFMNDKSSNRNRYMTGYTLAKQLSLYNGTVPLKKEPVM
TTITELVDAINFLAEHGVTESKQMTRLENKLIESFELAEESLQKLDDKIIQLNQLGKLILGEKLEDIDQE
FPVLKEVGDKDELTFEDIQNELSSAKLSRKLLHEKMESTVEKINELHAIQTVAEQKSSEENLGKL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 15830 | GenBank | WP_127843785 |
| Name | t4cp2_EQB74_RS16660_E7471|4 |
UniProt ID | _ |
| Length | 600 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 600 a.a. Molecular weight: 69128.94 Da Isoelectric Point: 5.9061
>WP_127843785.1 VirD4-like conjugal transfer protein, CD1115 family [Enterococcus faecium]
MQIYKKNIKNYLILSVLLFLIFYITGNFFWLLPGSDYTAKMYFLFTEELNRYIEMNWLNFVLTPSLMAFC
FGILGFLCGMLAYVRDNDRGVYRYGEEHGSARYATVEEMSRFADRDPEKNIILTKNACMGISNRRLDLKY
QKNKNDVVIGGPGSGKTYTFIKPNIMQMNASFIVTDPKGLLVHETGSMLEKNGYKIKIFDLLNLSNSDSF
NVFNYIHTELDVDRVLEFITEGTKKSEQTGEDFWIQAEALLIRSFIAFLWFDGKDNDYMPNLSMVGDMLR
HTERKDPKVASPVENWFEEQNEIRPNNYAYKQWKLFNDTYKAETRASVLAMAAGRYSVFDHDQVVDMVRE
DTMEIEKWNEEKTAVFISIPETSSAYNFLAAIFMATIMETLRSKVDAVLQGKKLLGKGKELLHVRFLIDE
FANIGRIPNIDKALATFRSREMSIVIVLQALNQLEAMYKNSWQTLVNTCDSLLFLGGDEKSTTEYLSKRA
GKQTISLRKHSKSKSGGSESRDKTGRDLLMSDEIGRLGNGKALLFISGQHVFKDDKYTVNDHKNASLLAN
DVYDENWYNYRRYRNEEEELLDMVKPENIIDHGVVGEEVS
MQIYKKNIKNYLILSVLLFLIFYITGNFFWLLPGSDYTAKMYFLFTEELNRYIEMNWLNFVLTPSLMAFC
FGILGFLCGMLAYVRDNDRGVYRYGEEHGSARYATVEEMSRFADRDPEKNIILTKNACMGISNRRLDLKY
QKNKNDVVIGGPGSGKTYTFIKPNIMQMNASFIVTDPKGLLVHETGSMLEKNGYKIKIFDLLNLSNSDSF
NVFNYIHTELDVDRVLEFITEGTKKSEQTGEDFWIQAEALLIRSFIAFLWFDGKDNDYMPNLSMVGDMLR
HTERKDPKVASPVENWFEEQNEIRPNNYAYKQWKLFNDTYKAETRASVLAMAAGRYSVFDHDQVVDMVRE
DTMEIEKWNEEKTAVFISIPETSSAYNFLAAIFMATIMETLRSKVDAVLQGKKLLGKGKELLHVRFLIDE
FANIGRIPNIDKALATFRSREMSIVIVLQALNQLEAMYKNSWQTLVNTCDSLLFLGGDEKSTTEYLSKRA
GKQTISLRKHSKSKSGGSESRDKTGRDLLMSDEIGRLGNGKALLFISGQHVFKDDKYTVNDHKNASLLAN
DVYDENWYNYRRYRNEEEELLDMVKPENIIDHGVVGEEVS
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 46081..54587
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EQB74_RS16585 | 41593..42198 | + | 606 | WP_000599739 | protein adenylyltransferase Fic | - |
| EQB74_RS16590 | 42214..42795 | + | 582 | WP_127843781 | recombinase family protein | - |
| EQB74_RS16595 | 42829..43509 | - | 681 | WP_001015311 | IS6-like element IS1216 family transposase | - |
| EQB74_RS16600 | 43590..44384 | + | 795 | WP_232044221 | hypothetical protein | - |
| EQB74_RS16605 | 44464..44859 | + | 396 | WP_033628807 | hypothetical protein | - |
| EQB74_RS16610 | 45187..45291 | + | 105 | Protein_57 | recombinase family protein | - |
| EQB74_RS16615 | 45339..46019 | - | 681 | WP_001015311 | IS6-like element IS1216 family transposase | - |
| EQB74_RS16620 | 46081..46476 | + | 396 | WP_197724945 | hypothetical protein | prgHb |
| EQB74_RS16625 | 46476..46826 | + | 351 | WP_086328746 | PrgI family mobile element protein | prgIc |
| EQB74_RS16630 | 46780..49155 | + | 2376 | WP_086328747 | VirB4-like conjugal transfer ATPase, CD1110 family | virb4 |
| EQB74_RS16635 | 49167..51827 | + | 2661 | WP_127843782 | phage tail tip lysozyme | prgK |
| EQB74_RS16640 | 51845..52570 | + | 726 | WP_127843783 | hypothetical protein | prgL |
| EQB74_RS16645 | 52554..52799 | + | 246 | WP_010731382 | hypothetical protein | - |
| EQB74_RS16650 | 52823..54079 | + | 1257 | WP_127843784 | hypothetical protein | - |
| EQB74_RS16655 | 54102..54587 | + | 486 | WP_086328751 | DUF3801 domain-containing protein | gbs1365 |
| EQB74_RS16660 | 54587..56389 | + | 1803 | WP_127843785 | VirD4-like conjugal transfer protein, CD1115 family | - |
| EQB74_RS16665 | 56524..57276 | + | 753 | WP_002332613 | Fic family protein | - |
| EQB74_RS16670 | 57343..57537 | + | 195 | WP_086328753 | hypothetical protein | - |
Host bacterium
| ID | 21879 | GenBank | NZ_LR135334 |
| Plasmid name | E7471|4 | Incompatibility group | - |
| Plasmid size | 72466 bp | Coordinate of oriT [Strand] | 63654..63696 [+] |
| Host baterium | Enterococcus faecium isolate E7471 |
Cargo genes
| Drug resistance gene | aac(6')-Im, aph(2'')-Ib |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA21 |