Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 121451
• Name: oriT_E4456|2
• Organism: Enterococcus faecium isolate E4456
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_LR135483 (142639..142676 [+], 38 nt)
• oriT length: 38 nt
• IRs (inverted repeats): 14..22, 29..37 (TAAAGTATA..TATACTTTA) 2..8, 13..19 (ACTTTAT..ATAAAGT)
• Location of nic site: 27..28
• Conserved sequence flanking the nic site: GTGTGTTATA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 38 nt

>oriT_E4456|2
CACTTTATGAATATAAAGTATAGTGTGTTATACTTTAC

oriT secondary structure

Predicted by RNAfold.

T4CP

t4cp2_EQB55_RS13650_E4456|2

• ID: 15829
• GenBank: WP_010725834
• Name: t4cp2_EQB55_RS13650_E4456|2
• UniProt ID: _
• Length: 869 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 869 a.a.
• Molecular weight: 100785.53 Da
• Isoelectric Point: 6.3146

>WP_010725834.1 VirD4-like conjugal transfer protein, CD1115 family [Enterococcus faecium]
MRVRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRISQKIFYSGLAYLPLNYVVTRIPEAFHR
FEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWNPLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLAD
GQKGDNRLKTLKEIQQIYPQIPESKGGFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLAT
IDNLSRAERQSSMVVNDPKTELYVASKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDIDTAT
QLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNNLVELLSEIGGFNYQDP
DNEFKQLNALDEFMNQLPPGNIAKKQYRATKIGSDKAKGNILSTAITGLNPFTLTKIAKMTSQSTIDYKS
IGFPKYLDLKLHESLLNQRIVVEFYHQGKKFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYDHRGKEYV
AWYEFSQRVLKDEEGNIVYRKKRGETHLPELEKEAVLTLDEDKSNLLVESIRMHYTDKPMAIFMLTPDYD
PSNHIIVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNMLWDLFIQS
YQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENSTINSLGMNTHINRNVDSDR
ILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNHGETNLPYAYQYIEDFNPSWDINDIDIHCTHA
DLDLKALQIDYDYFLPDIYARRLYHNMHDQIEEEAKENIFIKIVQSLDNSTAQKLLIQFYEEKQIDRLQE
YLEKLRQNEEITEVIRKRLLLEAEKAVKE

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (189-421)

• TraM recognition site of TraD and TraG (TraG-D_C) (593-709)

Protein structure

No available structure.

Host bacterium

• ID: 21878
• GenBank: NZ_LR135483
• Plasmid name: E4456|2
• Incompatibility group: -
• Plasmid size: 221062 bp
• Coordinate of oriT [Strand]: 142639..142676 [+]
• Host baterium: Enterococcus faecium isolate E4456

Cargo genes

• Drug resistance gene: aph(3')-III, ant(6)-Ia, erm(B), tet(L), lnu(B), lsa(E)
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA21