Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 121427 |
| Name | oriT_FERM P-14531|1  |
| Organism | Vibrio rumoiensis strain FERM P-14531 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_AP018687 (140117..140267 [-], 151 nt) |
| oriT length | 151 nt |
| IRs (inverted repeats) | 61..67, 73..79 (ATGCTCA..TGAGCAT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 151 nt
>oriT_FERM P-14531|1
TGGTCGATGTGAACCCTTTTGACCGGGTTATGAATGAATTGAAAAATCGTGGCCGCAAAAATGCTCATATCCTGAGCATCCTTCAATTCGATTGGCCTGCATCAGAGGTCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGTATTAA
TGGTCGATGTGAACCCTTTTGACCGGGTTATGAATGAATTGAAAAATCGTGGCCGCAAAAATGCTCATATCCTGAGCATCCTTCAATTCGATTGGCCTGCATCAGAGGTCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGTATTAA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 13509 | GenBank | WP_089140453 |
| Name | mobH_VRUMOI_RS18635_FERM P-14531|1 |
UniProt ID | _ |
| Length | 989 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 989 a.a. Molecular weight: 109289.47 Da Isoelectric Point: 4.7510
>WP_089140453.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIEATPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWATQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHGIERYFIRWRDKRHKRHEQFSL
LAWHRIIPAETQEFLSKSGPSIIEAMLEAISGTTVNQPVTRLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISQDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEATGSLKILMLRLESNDLVFTNEPPSAVAAEVVGDVEDAEIE
FVDPDESDDDQDEGGVSLNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSTEPEDTPAQGAAETKDSPKAP
SATTPSKGKQVKAKPKKDTEEQTEKPEAKEELSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDAF
NASTDACIADEESLKNSNEAELEQPKPDFVPEEQNSLQDDDFPMFSGSDEPPSWAIEPLPMLAVESEEAS
ATTEMSQANIPNQQEKDSKTLLSEMLAGYGEASALLEQAIMPVLEGKKTLGEVLCLMKGQAVILYPEGAR
SLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAQQLSDAVVAAIKEVEASMGGYQDVFELVS
PPDSDVRKSKSAPKQQSQPMQKKNQQQKSDAGSGQAAPAKDTKGKAAQKPQKEKKGDTSSVVAGHESKPI
EEKQNVARLPKREAQPEAVVVTKVEREKELGHIEVREREEQEVREFEPPKAKTNPKDIKDEDFLPPGVTP
EKAILILKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGINKHILCGMLSRAQKRPLLKKRQG
KLYLEVDET
MLKALNKLFGGRSGVIEATPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWATQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHGIERYFIRWRDKRHKRHEQFSL
LAWHRIIPAETQEFLSKSGPSIIEAMLEAISGTTVNQPVTRLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISQDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEATGSLKILMLRLESNDLVFTNEPPSAVAAEVVGDVEDAEIE
FVDPDESDDDQDEGGVSLNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSTEPEDTPAQGAAETKDSPKAP
SATTPSKGKQVKAKPKKDTEEQTEKPEAKEELSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPFDAF
NASTDACIADEESLKNSNEAELEQPKPDFVPEEQNSLQDDDFPMFSGSDEPPSWAIEPLPMLAVESEEAS
ATTEMSQANIPNQQEKDSKTLLSEMLAGYGEASALLEQAIMPVLEGKKTLGEVLCLMKGQAVILYPEGAR
SLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAQQLSDAVVAAIKEVEASMGGYQDVFELVS
PPDSDVRKSKSAPKQQSQPMQKKNQQQKSDAGSGQAAPAKDTKGKAAQKPQKEKKGDTSSVVAGHESKPI
EEKQNVARLPKREAQPEAVVVTKVEREKELGHIEVREREEQEVREFEPPKAKTNPKDIKDEDFLPPGVTP
EKAILILKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGINKHILCGMLSRAQKRPLLKKRQG
KLYLEVDET
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 15817 | GenBank | WP_022635545 |
| Name | traC_VRUMOI_RS18555_FERM P-14531|1 |
UniProt ID | _ |
| Length | 814 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 814 a.a. Molecular weight: 92275.44 Da Isoelectric Point: 6.0244
>WP_022635545.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIVTIKKKLQETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSVGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKATLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTDRISAKTNKGTYDNGLIQ
DLKLFVTCKVPINNNNPSESELQNLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
AVDWEMDKPICEQVFDYGTDVEVSKNGLRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKISYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWD
EKGKEMKVDDIAERCLEGEDMRLRDIGQQLYAFTSQGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHLR
QVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSTFMEHAYRKFRKYGGSVVIATQSINDLYE
NAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRLI
VGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRNRKQAA
MIVTIKKKLQETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSVGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKATLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTDRISAKTNKGTYDNGLIQ
DLKLFVTCKVPINNNNPSESELQNLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
AVDWEMDKPICEQVFDYGTDVEVSKNGLRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKISYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWD
EKGKEMKVDDIAERCLEGEDMRLRDIGQQLYAFTSQGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHLR
QVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSTFMEHAYRKFRKYGGSVVIATQSINDLYE
NAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRLI
VGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRNRKQAA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 15818 | GenBank | WP_022635536 |
| Name | traD_VRUMOI_RS18630_FERM P-14531|1 |
UniProt ID | _ |
| Length | 621 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 621 a.a. Molecular weight: 69430.92 Da Isoelectric Point: 7.1173
>WP_022635536.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLRGRDLEFISITELQKYIKDTPEDMWLGSGFLWENRHAQRVFEILKRDWTSIVGKESTVKKVVRKI
QGKRKELPIGQPWIHGVEPKEEKLMQPLKHTEGHTLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIRAVQAYSERVRPDWEAEAAPFLEKVKNGSREKIAFA
LMRFYYEIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSTDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLRGRDLEFISITELQKYIKDTPEDMWLGSGFLWENRHAQRVFEILKRDWTSIVGKESTVKKVVRKI
QGKRKELPIGQPWIHGVEPKEEKLMQPLKHTEGHTLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIRAVQAYSERVRPDWEAEAAPFLEKVKNGSREKIAFA
LMRFYYEIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSTDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 116981..142183
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| VRUMOI_RS18495 | 112109..113014 | - | 906 | WP_089140447 | hypothetical protein | - |
| VRUMOI_RS18500 | 113025..113993 | - | 969 | WP_022635552 | AAA family ATPase | - |
| VRUMOI_RS18505 | 114213..114398 | - | 186 | WP_154398366 | hypothetical protein | - |
| VRUMOI_RS18510 | 114703..115023 | - | 321 | WP_013141561 | hypothetical protein | - |
| VRUMOI_RS18515 | 115317..115958 | + | 642 | WP_071681740 | hypothetical protein | - |
| VRUMOI_RS18520 | 116080..116943 | + | 864 | WP_022635550 | hypothetical protein | - |
| VRUMOI_RS18525 | 116981..119797 | - | 2817 | WP_022635549 | conjugal transfer mating pair stabilization protein TraN | traN |
| VRUMOI_RS18530 | 119901..120884 | - | 984 | WP_231398180 | TraU family protein | traU |
| VRUMOI_RS18535 | 120905..121576 | - | 672 | WP_071681744 | EAL domain-containing protein | - |
| VRUMOI_RS18540 | 121573..122838 | - | 1266 | WP_089140448 | TrbC family F-type conjugative pilus assembly protein | traW |
| VRUMOI_RS18545 | 122801..123331 | - | 531 | WP_089140449 | S26 family signal peptidase | - |
| VRUMOI_RS18550 | 123328..123645 | - | 318 | WP_013141553 | hypothetical protein | - |
| VRUMOI_RS18555 | 123660..126104 | - | 2445 | WP_022635545 | type IV secretion system protein TraC | virb4 |
| VRUMOI_RS18560 | 126101..126808 | - | 708 | WP_089140450 | DsbC family protein | trbB |
| VRUMOI_RS18565 | 126957..132488 | - | 5532 | WP_089140451 | Ig-like domain-containing protein | - |
| VRUMOI_RS18570 | 132691..133074 | - | 384 | WP_089140452 | TraA family conjugative transfer protein | - |
| VRUMOI_RS18575 | 133087..133665 | - | 579 | WP_010845916 | type IV conjugative transfer system lipoprotein TraV | traV |
| VRUMOI_RS18580 | 133662..134981 | - | 1320 | WP_071681777 | TraB/VirB10 family protein | traB |
| VRUMOI_RS18585 | 134981..135898 | - | 918 | WP_022635540 | type-F conjugative transfer system secretin TraK | traK |
| VRUMOI_RS18590 | 135882..136508 | - | 627 | WP_022635539 | TraE/TraK family type IV conjugative transfer system protein | traE |
| VRUMOI_RS18595 | 136505..136786 | - | 282 | WP_000805625 | type IV conjugative transfer system protein TraL | traL |
| VRUMOI_RS18600 | 136934..137296 | - | 363 | WP_231897578 | hypothetical protein | - |
| VRUMOI_RS19400 | 137308..137451 | - | 144 | WP_001275801 | hypothetical protein | - |
| VRUMOI_RS18605 | 137633..138295 | - | 663 | WP_022635538 | hypothetical protein | - |
| VRUMOI_RS18610 | 138295..138672 | - | 378 | WP_013141541 | hypothetical protein | - |
| VRUMOI_RS18615 | 138682..139128 | - | 447 | WP_071681752 | hypothetical protein | - |
| VRUMOI_RS18620 | 139137..139766 | - | 630 | WP_022635537 | DUF4400 domain-containing protein | tfc7 |
| VRUMOI_RS18625 | 139723..140268 | - | 546 | WP_023044870 | hypothetical protein | - |
| VRUMOI_RS18630 | 140318..142183 | - | 1866 | WP_022635536 | conjugative transfer system coupling protein TraD | virb4 |
| VRUMOI_RS18635 | 142180..145149 | - | 2970 | WP_089140453 | MobH family relaxase | - |
| VRUMOI_RS18640 | 145317..145934 | + | 618 | WP_071681756 | hypothetical protein | - |
| VRUMOI_RS18645 | 145916..146149 | + | 234 | WP_071681758 | hypothetical protein | - |
Host bacterium
| ID | 21854 | GenBank | NZ_AP018687 |
| Plasmid name | FERM P-14531|1 | Incompatibility group | IncA/C2 |
| Plasmid size | 159324 bp | Coordinate of oriT [Strand] | 140117..140267 [-] |
| Host baterium | Vibrio rumoiensis strain FERM P-14531 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | katA |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |