Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   121417
Name   oriT_pECL-14-60-NDM-1-IncAC in_silico
Organism   Enterobacter hormaechei strain ECL-14-60
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_MZ836805 (87210..87360 [+], 151 nt)
oriT length   151 nt
IRs (inverted repeats)      93..98, 106..111  (TGGCCT..AGGCCA)
 12..17, 24..29  (AACCCT..AGGGTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 151 nt

>oriT_pECL-14-60-NDM-1-IncAC
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   13502 GenBank   WP_001337757
Name   mobH_LUX26_RS00575_pECL-14-60-NDM-1-IncAC insolico UniProt ID   A0A709FRI2
Length   992 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 992 a.a.        Molecular weight: 109581.85 Da        Isoelectric Point: 4.8956

>WP_001337757.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEADDGDDQEEGEAALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGPTESSK
PDAGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPF
DAFNASAETTSTDATNSEIPDVAMPGKQEEQPKQDFVPQEQNSLQGDDFPMFGGSDEPPSWAIEPLPMLT
DAPEQPTHTPEMPHTDNVNQHEKDAKTLLVEMLSGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVI
LYPDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQ
DAFELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKASPEQKAKGKDSQPQPKEKKVDVTSPVEEQQ
RKPVQEKQNVARLPKREAQPVAPEPKVEREKELGHVEVREREDPEVREFEPPKAKTNPKDINAEDFLPSG
VTPQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKK
RQGKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure


Source ID Structure
AlphaFold DB A0A709FRI2


T4CP

ID   15807 GenBank   WP_000178856
Name   traD_LUX26_RS00580_pECL-14-60-NDM-1-IncAC insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69197.66 Da        Isoelectric Point: 6.9403

>WP_000178856.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPDDMWLGSGFLWENRHAQRVFEILKRDWTSIVGRESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVMPDWEAEAAAYLEKAKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-297)

  Protein structure



No available structure.



ID   15808 GenBank   WP_000637386
Name   traC_LUX26_RS00660_pECL-14-60-NDM-1-IncAC insolico UniProt ID   _
Length   815 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 815 a.a.        Molecular weight: 92590.87 Da        Isoelectric Point: 5.9222

>WP_000637386.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIVTIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSAGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTTLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTERIFAKTNKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQQLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSKGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRQQAA

  Protein domains


Predicted by InterproScan.

TraG P-loop domain (P-loop_TraG)

(449-809)

Family of unknown function (DUF5934) (DUF5934)

(284-434)

TraC protein (TraC_F_IV)

(21-255)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 85294..110485

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LUX26_RS00565 81319..81552 - 234 WP_001191892 hypothetical protein -
LUX26_RS00570 81534..82151 - 618 WP_001249396 hypothetical protein -
LUX26_RS00575 82319..85297 + 2979 WP_001337757 MobH family relaxase -
LUX26_RS00580 85294..87159 + 1866 WP_000178856 conjugative transfer system coupling protein TraD virb4
LUX26_RS00585 87170..87754 + 585 WP_000332866 hypothetical protein -
LUX26_RS00590 87711..88340 + 630 WP_000743450 DUF4400 domain-containing protein tfc7
LUX26_RS00595 88350..88796 + 447 WP_000122506 hypothetical protein -
LUX26_RS00600 88806..89183 + 378 WP_000869261 hypothetical protein -
LUX26_RS00605 89183..89845 + 663 WP_001231463 hypothetical protein -
LUX26_RS00610 90026..90169 + 144 WP_001275801 hypothetical protein -
LUX26_RS00615 90169..90546 + 378 WP_000509509 hypothetical protein -
LUX26_RS00620 90691..90972 + 282 WP_000805625 type IV conjugative transfer system protein TraL traL
LUX26_RS00625 90969..91595 + 627 WP_001049716 TraE/TraK family type IV conjugative transfer system protein traE
LUX26_RS00630 91579..92496 + 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
LUX26_RS00635 92496..93809 + 1314 WP_024131605 TraB/VirB10 family protein traB
LUX26_RS00640 93806..94384 + 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
LUX26_RS00645 94388..94780 + 393 WP_000479535 TraA family conjugative transfer protein -
LUX26_RS00650 94981..100512 + 5532 WP_000606833 Ig-like domain-containing protein -
LUX26_RS00655 100661..101368 + 708 WP_001259347 DsbC family protein -
LUX26_RS00660 101365..103812 + 2448 WP_000637386 type IV secretion system protein TraC virb4
LUX26_RS00665 103827..104144 + 318 WP_000351984 hypothetical protein -
LUX26_RS00670 104141..104671 + 531 WP_001010738 S26 family signal peptidase -
LUX26_RS00675 104634..105899 + 1266 WP_000621288 TrbC family F-type conjugative pilus assembly protein traW
LUX26_RS00680 105896..106567 + 672 WP_001337754 EAL domain-containing protein -
LUX26_RS00685 106567..107571 + 1005 WP_043940352 TraU family protein traU
LUX26_RS00690 107675..110485 + 2811 WP_001909286 conjugal transfer mating pair stabilization protein TraN traN
LUX26_RS00695 110524..111384 - 861 WP_000709517 hypothetical protein -
LUX26_RS00700 111507..112148 - 642 WP_000796664 hypothetical protein -
LUX26_RS00705 112442..112762 + 321 WP_000547566 hypothetical protein -
LUX26_RS00710 113066..113251 + 186 WP_001186917 hypothetical protein -
LUX26_RS00715 113471..114439 + 969 WP_000085162 AAA family ATPase -
LUX26_RS00720 114450..115358 + 909 WP_000739139 hypothetical protein -

Host bacterium

ID   21844 GenBank   NZ_MZ836805
Plasmid name   pECL-14-60-NDM-1-IncAC Incompatibility group   IncA/C2
Plasmid size   171038 bp Coordinate of oriT [Strand]   87210..87360 [+]
Host baterium   Enterobacter hormaechei strain ECL-14-60

Cargo genes

Drug resistance gene   aph(3')-Ia, aac(6')-Ib-cr, blaOXA-1, catB3, ARR-3, qacE, sul1, blaNDM-1, qnrA7, armA, msr(E), mph(E), sul2
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -