Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 121386
• Name: oriT_pNIB353_3
• Organism: Bacillus subtilis strain NIB353
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP089151 (4145..4167 [+], 23 nt)
• oriT length: 23 nt
• IRs (inverted repeats): 2..7, 17..22 (CCCCCC..GGGGGG) 1..6, 17..22 (CCCCCC..GGGGGG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 23 nt

>oriT_pNIB353_3
CCCCCCCACGCTAACAGGGGGGT

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobV_LRO88_RS23995_pNIB353_3

• ID: 13489
• GenBank: WP_231834329
• Name: mobV_LRO88_RS23995_pNIB353_3
• UniProt ID: _
• Length: 483 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 483 a.a.
• Molecular weight: 56855.39 Da
• Isoelectric Point: 9.7377

>WP_231834329.1 MULTISPECIES: MobV family relaxase [Bacillus]
MANYAVIRMEKYKKDRLNGTQKHNQREFQKSKNENIDRGRSHLNYDLINGQPISYAQAVREKIEGRVKRK
VRDDAVLVSEFLITASPDYMNGLSETEQRRYFEAAADHLKEKYSAENMLYATVHMDEATPHMHVGIVPIT
EDGRLSAKDFFNGKLKMKAIQDDFHRHMVKSGFDLERGEPSEKKHENVHQYKINQRKAELERLNAEIALK
EKRKAELEQQNRAVESVVSAKKEALTTKTQERRLPTVEYEKAGLFKKDKVTVPQHELENLYAYAEDQGKM
AAQLSRQLEAETEEKERWKSIAVEERERADSKDKMLLDLQTTMNEEVEKAKRGMRRKLTKEFTEEQREEL
RQEVKEELSALKTENSELRTENKDLKRLTLSLSEQKDNEVKKHNETKKSLKAERGRYGELLNFAQEQNKE
FQVLNKENKTLRERVAVLEQWKDKMVQWAKEKLPRVRKLAASFFRTAGMPREANKYKDHELER

Protein domains

Predicted by InterproScan.

• Plasmid recombination enzyme (Mob_Pre) (1-192)

Protein structure

No available structure.

Host bacterium

• ID: 21813
• GenBank: NZ_CP089151
• Plasmid name: pNIB353_3
• Incompatibility group: Col
• Plasmid size: 8494 bp
• Coordinate of oriT [Strand]: 4145..4167 [+]
• Host baterium: Bacillus subtilis strain NIB353

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -