Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 121374 |
| Name | oriT_pSMC1  |
| Organism | Serratia marcescens SM39 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_AP013064 (11914..12024 [-], 111 nt) |
| oriT length | 111 nt |
| IRs (inverted repeats) | 71..78, 81..88 (CACCATGC..GCATGGTG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 111 nt
>oriT_pSMC1
CCGGCTGGCCCCGCAGGGCAGGATGCCCCGTTGAGCGCCGCAGGCGCGAATAAGGGGAAGTGAAGAGGATCACCATGCTTGCATGGTGGGCCTACTTCACACATCCTGCCC
CCGGCTGGCCCCGCAGGGCAGGATGCCCCGTTGAGCGCCGCAGGCGCGAATAAGGGGAAGTGAAGAGGATCACCATGCTTGCATGGTGGGCCTACTTCACACATCCTGCCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 13483 | GenBank | WP_041038529 |
| Name | traI_SM39_RS24375_pSMC1 |
UniProt ID | _ |
| Length | 609 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 609 a.a. Molecular weight: 66661.54 Da Isoelectric Point: 10.6884
>WP_041038529.1 TraI/MobA(P) family conjugative relaxase [Serratia marcescens]
MIAKHVPMRSLGKSDFAGLVRYVTDAQSKDHRLGQVQVTNCAADTVRDAITEVLATQQANTRAKGDKTYH
LIVSFRAGEQPSADTLRQIEARICAGLGFAEHQRVSAVHTDTDNLHIHIAINKIHPTRGTMQEPYYSHRA
LADLCATLERDYGLERDNHEPRQRGAAARAGDLEQHAGVESLVGWIKRECLDEIKGAASWAELHQVMRDN
GLALRARGNGLVIEAGDGTIIKASTLARDLSKPALEKRLGTFEASPERQATPARRSYQKGRPMRLRGGLD
TTELYARYKGAQQTLTAARAGVLAEAKRRKDRAIEHAKKAAATRRAAIKHLTDGRLTKKLLYAQASAALR
ADLEAIHKRHDQERAAIYNEHRRRPWADWLKQQAMAGDGQALDALRAREAAQGLKGDTIQGDGQAKPGHA
PVIDNITKKGTIIFRAGSSAVRDDGERLAVSSQADRAGVAAALRLAMERYGERITVSGSVEFKAAVIRAA
VDAQLPITFADPALESRRQAEATKAQAKRRPGVPPVGQEPPPHRRHGLRTLGQLDGLHIEGEAQRPAQAP
TQATPTPPPAKVQQPARPAPSELEQREARLRAQMEAKKAKRQEGKKRGR
MIAKHVPMRSLGKSDFAGLVRYVTDAQSKDHRLGQVQVTNCAADTVRDAITEVLATQQANTRAKGDKTYH
LIVSFRAGEQPSADTLRQIEARICAGLGFAEHQRVSAVHTDTDNLHIHIAINKIHPTRGTMQEPYYSHRA
LADLCATLERDYGLERDNHEPRQRGAAARAGDLEQHAGVESLVGWIKRECLDEIKGAASWAELHQVMRDN
GLALRARGNGLVIEAGDGTIIKASTLARDLSKPALEKRLGTFEASPERQATPARRSYQKGRPMRLRGGLD
TTELYARYKGAQQTLTAARAGVLAEAKRRKDRAIEHAKKAAATRRAAIKHLTDGRLTKKLLYAQASAALR
ADLEAIHKRHDQERAAIYNEHRRRPWADWLKQQAMAGDGQALDALRAREAAQGLKGDTIQGDGQAKPGHA
PVIDNITKKGTIIFRAGSSAVRDDGERLAVSSQADRAGVAAALRLAMERYGERITVSGSVEFKAAVIRAA
VDAQLPITFADPALESRRQAEATKAQAKRRPGVPPVGQEPPPHRRHGLRTLGQLDGLHIEGEAQRPAQAP
TQATPTPPPAKVQQPARPAPSELEQREARLRAQMEAKKAKRQEGKKRGR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
| ID | 7439 | GenBank | WP_011997489 |
| Name | WP_011997489_pSMC1 |
UniProt ID | A0A482F300 |
| Length | 125 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
Auxiliary protein sequence
Download Length: 125 a.a. Molecular weight: 13548.38 Da Isoelectric Point: 8.9596
>WP_011997489.1 MULTISPECIES: conjugal transfer transcriptional regulator TraJ [Pseudomonadota]
MEANENKVTRKGSTPIKVYCLPDERAAIEANAQAAGMSTSAYLLAVGQGYQVKGVTDFENVRDLVRVNGD
LGRLGGLLKLWLTDDPRTARFGDDTIRALLARIEATQDKISTIAESVVRPRGGRS
MEANENKVTRKGSTPIKVYCLPDERAAIEANAQAAGMSTSAYLLAVGQGYQVKGVTDFENVRDLVRVNGD
LGRLGGLLKLWLTDDPRTARFGDDTIRALLARIEATQDKISTIAESVVRPRGGRS
Protein domains
No domain identified.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A482F300 |
Host bacterium
| ID | 21801 | GenBank | NZ_AP013064 |
| Plasmid name | pSMC1 | Incompatibility group | - |
| Plasmid size | 41517 bp | Coordinate of oriT [Strand] | 11914..12024 [-] |
| Host baterium | Serratia marcescens SM39 |
Cargo genes
| Drug resistance gene | aadA2, qacE, sul1, blaIMP-1, blaCMY-8 |
| Virulence gene | - |
| Metal resistance gene | merE, merD, merA, merP, merT, merR |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |