Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 121344
• Name: oriT_18ISCm|unnamed3
• Organism: Carnobacterium maltaromaticum strain 18ISCm
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP045043 (60359..60394 [-], 36 nt)
• oriT length: 36 nt
• IRs (inverted repeats): _
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 36 nt

>oriT_18ISCm|unnamed3
CACACGTATGTGGGTACAGTTTCCCTTATGCTCTTT

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobP2_F9281_RS18780_18ISCm|unnamed3

• ID: 13468
• GenBank: WP_157456903
• Name: mobP2_F9281_RS18780_18ISCm|unnamed3
• UniProt ID: _
• Length: 413 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 413 a.a.
• Molecular weight: 48024.54 Da
• Isoelectric Point: 9.4699

>WP_157456903.1 MobP2 family relaxase [Carnobacterium maltaromaticum]
MAKGKPAIVMVAKFVVHSDAKFNSYVNYMDRTEAQRSEKFNEFNAFTFDGYHEYMENPYKSTGLFSKDYS
VMGDREKQLLKDKFTVAQENKSPMWQTVFSFDNAWLEEQGLMDGKVHYLNEDPIKEAVTLAMNEQMRLMK
MGDTANWTAAVHYNTDNVHVHVALVEEVPTRQKFIYGGEEQYKAKIPPKVMDRMKSTFSNSMVNRDMELA
RISYLIRTELKLGTGKDGLRDVRVRKTLTNLLNELPEDKRLWQYGNTAMIPFRNELNYVTDLILKDENPH
ALKELDGRLEDEMNFFKRLYGEGTKEFERFKEYKSNKLAELYKDNGNSILKELKVIQRNGGIEKVGQGEK
RRIQSSGKSFVSKKNVSAIKKAFGKNKQDYLGKLAYQRLQAEKENAAYQQKQNQETDRGYGYD

Protein domains

Predicted by InterproScan.

• MobL relaxases (MobL) (5-354)

Protein structure

No available structure.

T4CP

t4cp2_F9281_RS18840_18ISCm|unnamed3

• ID: 15776
• GenBank: WP_157456915
• Name: t4cp2_F9281_RS18840_18ISCm|unnamed3
• UniProt ID: _
• Length: 853 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 853 a.a.
• Molecular weight: 97224.62 Da
• Isoelectric Point: 4.8916

>WP_157456915.1 VirD4-like conjugal transfer protein, CD1115 family [Carnobacterium maltaromaticum]
MVIDKVSEIFQKFDQKKAQWIVATALFVIATLLGNLMSSGLKTLDNLFQLNPQTKTVSDFIGFHMIDFFP
LYVILYGIAGASFFVVWTQLKASYESMEQGNKGTARWTTLTELYEQYTQVPKLAKSDDETYPGLSGTLIA
QDGDVSFIDEGPAHNKITGRSRSGKDQTKVLPDLDLYSRAEEKPHIISVSTKYEVFAAAKERLEKRGYEV
SLFNLIHLERSLMFNPLELIKKAYMKGDVDEAIELCKTFSFPLYHNEEAKEPVWEETAMALVNAIILALC
YEFIEKSENPKLTEKYVTLYSAVMMLIELGATDKHGNTLLDKYFASLEPDNPARMEYSTVQFADGQMRSS
IFASTQAKLRNFIAPKVAKMMSKSNFGFDRFLGFEGAYTTLIVDEMADSEVDYRENPFKAFDLGSFKTGS
EVPLPIVEPVSSQSKMISGFAVPGSTVTVILPTLENATGEFEQLSVTATKSGTFSIKLEKQIESNQAVKV
NMAIIKPQAVFMVLPDYIETNYIIASTWIQQVYFVTSQYASENGDKLKKRIRAQLNEFGNLPAFSNIPSI
ISVGAGRGFLVDFYLQNDNQLRLKYDETHAKIIESEAMNTFFIFSKDKLTRDNFSEQLGETEYISKSRSG
GLFSFHKQYTETVETRPLMKSDELGRLMEGETVVDRSVKRRDLKGNKIVPHPIWNRGKTSYSYAYEYLTE
QFPNDRNWRQLDMPKVTDIPLKKYGEEFVKRIRRPLNKLLKEESEQENRVELDDIMSHEDAVLDKEYKGF
GTGESAAEFEDIQEVKLHVERKLMSEAFSSEDIYVMYQAAIGLYPEKTGEIDSMDYVEEYQDFLALPEHR
ELNEKYAKLGFFN

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (135-381)

• TraM recognition site of TraD and TraG (TraG-D_C) (541-658)

Protein structure

No available structure.

Host bacterium

• ID: 21771
• GenBank: NZ_CP045043
• Plasmid name: 18ISCm|unnamed3
• Incompatibility group: -
• Plasmid size: 107821 bp
• Coordinate of oriT [Strand]: 60359..60394 [-]
• Host baterium: Carnobacterium maltaromaticum strain 18ISCm

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA1