Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 121250
• Name: oriT_pHL28-2
• Organism: Staphylococcus sp. HL28
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP088220 (358..478 [-], 121 nt)
• oriT length: 121 nt
• IRs (inverted repeats): 53..60, 62..69 (TTGGGGAT..ATCCCCAA) 23..30, 35..42 (ATTTTTTC..GAAAAAAT) 1..8, 14..21 (AGTGGCTA..TAGCCACT)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 121 nt

>oriT_pHL28-2
AGTGGCTAGCAAATAGCCACTTATTTTTTCGACAGAAAAAATCCTAAGGGGCTTGGGGATTATCCCCAACAAGCTGGCGCGCCTGCCACGTTAGTGGCTAGCAAAGCCAATGCTTGCCAAA

oriT secondary structure

Predicted by RNAfold.

Relaxase

Relaxase_LPC11_RS12545_pHL28-2

• ID: 13421
• GenBank: WP_331275762
• Name: Relaxase_LPC11_RS12545_pHL28-2
• UniProt ID: _
• Length: 336 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 336 a.a.
• Molecular weight: 38545.15 Da
• Isoelectric Point: 6.2449

>WP_331275762.1 relaxase/mobilization nuclease domain-containing protein [Staphylococcus sp. HL28]
MATTKLSATKSTSRAINYAEKRAVEKSGLNCDVDYAKSSFKASRELYGKTDGNQGHVIIQSFKPNEVTSE
QCNQLGLELAEKMAPNHQVAVYTHSDTDHVHNHIVINSIDLETGKKFNNNKQALRNLRNFNDEVCREHGL
SVPEKDTARLRYTQTEKAITDPNTQSTAQYSWKDEIREAIDQSQATNMDEFKDHLNQHGIEIERVTPKSI
TYRHLAEDKKVRGRKLGEDYNKGGIEDGFERQIQRQQQTERATDTDVQLKQRTSSRDEATERDTGVTQAD
WDQFAQDTNELERSRQAAESARLAHEKAQRDREEREREKQASRTIINDDRDHGLEL

Protein domains

Predicted by InterproScan.

• Relaxase/Mobilisation nuclease domain (Relaxase) (9-242)

Protein structure

No available structure.

Host bacterium

• ID: 21678
• GenBank: NZ_CP088220
• Plasmid name: pHL28-2
• Incompatibility group: -
• Plasmid size: 8440 bp
• Coordinate of oriT [Strand]: 358..478 [-]
• Host baterium: Staphylococcus sp. HL28

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -