Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 121249
• Name: oriT_pWSM1455_4
• Organism: Rhizobium laguerreae strain WSM1455
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP088094 (127340..127368 [+], 29 nt)
• oriT length: 29 nt
• IRs (inverted repeats): 14..19, 24..29 (CGTCGC..GCGACG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 29 nt

>oriT_pWSM1455_4
AGGGCGCAATATACGTCGCTGTCGCGACG

oriT secondary structure

Predicted by RNAfold.

Relaxase

traA_RlegWSM1455_RS33510_pWSM1455_4

• ID: 13420
• GenBank: WP_231285911
• Name: traA_RlegWSM1455_RS33510_pWSM1455_4
• UniProt ID: _
• Length: 1550 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1550 a.a.
• Molecular weight: 171440.44 Da
• Isoelectric Point: 7.1457

>WP_231285911.1 Ti-type conjugative transfer relaxase TraA [Rhizobium laguerreae]
MAIMFVRAQVISRGTGRSIVSAAAYRHRARMMDEQAGTSFSYRGGRAELVHEELALPTQTPEWLRTAIDG
RTVAAASEALWNAVDAFEKRADAQLARELIIALPEELTRAENIALVREFVRDNLTSKGMVADWVYHDKDG
NPHIHLMTALRPLTQEGFGRKKVAVTGEDGAPLRVVTPDRPKGRIVYKLWTGDKETLQAWKVAWAETANR
HLALAGHEIRIDGRSYAEQGLDGIAQRHLGPEKAALSRRGAELYFAPADLARRQEMADRLLAEPELLLKQ
LGNERSTFDERDIAKALHRTVDDPTVFANVRARLMASDDLVMLKPQQVDAETGKASEPAVFTTRDILRVE
YDMARSAQVLSERRGFAVSAESVAAAIENVETADPEKSFRLDPEQVDAVRHVAGDNGIAAVVGLAGAGKS
TLLAAARVAWESGGRRVIGAALAGKAAEGLEDSSGIRSRTLASWELIWVNGHETLHRGDVLVIDEAGMVS
SQQMARVLKLVEQAGAKVVLVGDAMQLQSIQAGAAFRAITERIGFAELAGVRRQREDWAREASRLFARGE
TEKGLDAYAQQGRLVEAETRDEIIGRIVDDWTAARRQAIQASSTAGSDGRLRGNELLVLAHTNQDVKRLN
EALRSVMTGEGALGEGRSFRTERGAREFAAGDRMIFLENARFLEPRAPRLGPQYVKNGMLGTVVSTGDKR
GDTLLTVRLDNGRDVVISEDSYRNVDHGYAATIHKSQGATVERTFVLATGMMDQHLTYVSMTRHRDRADL
YAAKEDFEAKPEWGRKPHVDHAAGVTGELVETGQAKFRPNDEDADDSPYADVKTDDGSVHRLWGVSLPKA
LEEGGVSEGDTVTLRKDGVEKVKVQVPIVDEQTGQKRFEEREVDRNVWTAKRIETAAERQERIERESHRP
DVFKQLVERLSRSGAKTTTLDFESEASYQAHADDFARRRGIDTLSQAAAGMEEEVSRRWVWIAEKREQVA
ELWERASIALGFAIERERRVSYNEERIETQTVSDADAGRARHLMPSTTVFARSVDKDARLAQLASPAWKE
REAILRPLLEKIYRDPDAALSALNALSSDMSIEPRRLADDLAAAPDRLGRLRGSDLIVDGRAARDERNAA
ITALTELLPLARAHATEFRRNAERFDLREQQRRAHMSLPIPAPSRKAMKRLVEIEAVRRQGGDDAYKSAF
ALAAEDRSLVQEVKAVSEALTARFGWSAFTPNADTVAERNVIERMPEDLASTRREKLTRLFEAVKRFADE
QHLAERRNRSKIVAAASADQGMGTDKENAAVLPMLAAVTEFKTPVDEEARSRVLSGPLYRQQRAALAAVA
STIWRDPAGAVGKLEELLQKGFAADRIAAAVTNDPAAYGALRGSDRLMDRMLAAGRERKDAVQAMPEAAA
RLRALGSAYVNVLDGERRAIAEERRRMTVAIPGLTKAAEEVLMRLTAEAKNNGYKLSASAASLDPDIRRE
FAAVSRALDERFGRNAIIRGEKDLINHVPQAQHNALEAMREKLKVLQQAVRMESSEQIISERRQQAVNRG
RGIELRDALK

Protein domains

Predicted by InterproScan.

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1313-1405)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1045-1127)

• MobA/MobL family (MobA_MobL) (17-249)

• AAA domain (AAA_30) (391-578)

Protein structure

No available structure.

Host bacterium

• ID: 21677
• GenBank: NZ_CP088094
• Plasmid name: pWSM1455_4
• Incompatibility group: -
• Plasmid size: 277914 bp
• Coordinate of oriT [Strand]: 127340..127368 [+]
• Host baterium: Rhizobium laguerreae strain WSM1455

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -