Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 121248
• Name: oriT_pWSM1455_2
• Organism: Rhizobium laguerreae strain WSM1455
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP088092 (243498..243526 [+], 29 nt)
• oriT length: 29 nt
• IRs (inverted repeats): 14..19, 24..29 (CGTCGC..GCGACG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 29 nt

>oriT_pWSM1455_2
AGGGCGCAATATACGTCGCTGTCGCGACG

oriT secondary structure

Predicted by RNAfold.

Relaxase

traA_RlegWSM1455_RS30300_pWSM1455_2

• ID: 13419
• GenBank: WP_231285803
• Name: traA_RlegWSM1455_RS30300_pWSM1455_2
• UniProt ID: _
• Length: 1538 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1538 a.a.
• Molecular weight: 170235.42 Da
• Isoelectric Point: 7.3304

>WP_231285803.1 Ti-type conjugative transfer relaxase TraA [Rhizobium laguerreae]
MAIMFVRAQVISRGVGRSIVSAAAYRHRARMMDEQAGTSFSYRGGRAELVHEELALPTQTPEWLRTAIDG
RTVAAASEALWNAVDAFEKRADAQLARELIIALPEELTRAENIALVREFVRDNLTSKGMVADWVYHDKDG
NPHIHLMTALRPLTQEGFGRKKVAVTGEDGAPLRVVTPDRPKGRIVYKLWAGDKETMKAWKIAWAETANR
HLALAGHEIRIDGRSYAEQGLDGIAQKHLGPEKAALSRRGATMYFAPADLARRQEMADRLLAEPEFLLKR
LGNERSTFDERDIAKALHRTVDDPTVFANIRARLMASDDLIMLKPQQVDAETGKVAEPAVFTTREILRIE
YDMARSAQVLSEQKGFAVPSRYFAAAVKSVEAGDPDKPFKLDAEQINAISHITGDSGIAAVVGLAGAGKS
TLLAAARVAWESGGRRVIGAALAGKAAEGLEDSSGISSRTLTSWELIWANGHETLHRGDVLVIDEAGMVS
SQQMARVLKLVEQAGAKVVLVGDAMQLQPIQAGAAFRAITERIGFAELAGVRRQREDWAREASRLFARGE
VEKGLDAYAGHGHLVEAETRAETIDRIVADWTEARKQAIRNSTVVADDGRLRGDELLVLAHTNQDVKRLN
EALRSVMSGEGALGEGRSFQTERGAREFAAGDRIIFLENARFLEPRARRLGPQYVKNGMLGTVVSTGDKR
GEVLLSVRLDNGRDVVLSEDSYRNVDHGYAATIHKSQGATVERTFVLATGMMDQHLTYVSMTRHRDRADL
YAAKEDFEARPEWGRKPRVDHAAGVTGELVETGQAKFRPNDEDADDSPYADVKTDDGSVHRLWGVSLPKA
LEEGGVSEGDTVTLRKDGVEKVKVQVPIVDEQTGQKRFEEREVDRNVWTAKRIETAAERQERIERESHRP
ELFKQLVERLSRSGAKTTTLDFESEAGYQAHADDFARRRGLDTLSQAAAGMEEEVSRRWAWIAEKREQVA
ELWERASVALGFAIERERRVSYNEARIETQTVSDADTGRARYLIAPTTGFARTVAEDARLAQLSSPAWTE
REAILRPLLEKIYRDPDAALVALNALSSDASIEPRRLADDLVVAPDRLGRLRGSDLIVDGRAARDERNAA
ITALTELLPLARAHATEFRRNGERFDLGEQQRRTHMSLPIPALSEKAMARLTEIEAVRERGGDDAYKTAF
ALVTEDRSIVQEVKAVSEALTARFGWSAFTPNADAVAERNMVERMPEDLASTRREKLTQLFKAVKRFADE
QHLAERRDRSKIVAGASVDPEKENVPMLAAVTEFKTPVDEEARSRVLSGPLYRQQRAALATVASMIWRDP
AGAVGKIEELLQKGFAADRIAAAVTNDPAAYGALRGSDRLMDRMLATGRERKEAVQAVPEAAARLRALGS
TYVNVFAAERQVVGEERRRMTVAIPGLSKAAEDVLMRLTAEAKNNGRKRNASAVSLDPTIRQEFAAVSTA
LDERFGRNAILRDEKDLINRVPPVQRRAFEAMQERLRILQTTVRMESSDKIVSERRQRAVGRGRGIGF

Protein domains

Predicted by InterproScan.

• AAA domain (AAA_30) (391-578)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1306-1399)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1055-1127)

• MobA/MobL family (MobA_MobL) (17-249)

Protein structure

No available structure.

Host bacterium

• ID: 21676
• GenBank: NZ_CP088092
• Plasmid name: pWSM1455_2
• Incompatibility group: -
• Plasmid size: 521923 bp
• Coordinate of oriT [Strand]: 243498..243526 [+]
• Host baterium: Rhizobium laguerreae strain WSM1455

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: actP
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: -