Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 121245
• Name: oriT_pSU303_2
• Organism: Rhizobium sp. SU303
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP088097 (240404..240432 [+], 29 nt)
• oriT length: 29 nt
• IRs (inverted repeats): 14..19, 24..29 (CGTCGC..GCGACG)
• Location of nic site: _
• Conserved sequence flanking the nic site: _
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 29 nt

>oriT_pSU303_2
AGGGCGCAATATACGTCGCTGTCGCGACG

oriT secondary structure

Predicted by RNAfold.

Relaxase

traA_RlegSU303_RS30090_pSU303_2

• ID: 13416
• GenBank: WP_231284014
• Name: traA_RlegSU303_RS30090_pSU303_2
• UniProt ID: _
• Length: 1545 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 1545 a.a.
• Molecular weight: 170873.97 Da
• Isoelectric Point: 6.9969

>WP_231284014.1 Ti-type conjugative transfer relaxase TraA [Rhizobium sp. SU303]
MAIMFVRAQVISRGAGRSIVSAAAYRHRARMMDEQAGTSFSYRGGASELVHEELTLPDETPTWLRRAIDG
RSVAGTSEVLWNAVDVFEKRADAQLARELIIALPEELTRAENIALVREFVRDNLTSKGMVADWVYHDKDG
NPHIHLMTTLRPLTQEGFGRKKVAVTGEDGAPLRVVTPDRPKGRIVYKLWAGDKETMKTWKVAWAETANR
HLALAGHEIRLDGRSYAEQGLDGIAQKHLGPEKAALSRRGAEMYFAPADLARRQEMADRLLADPELLLKQ
LGNERSTFDERDIAKALHRTVDDPADFANIRARLMASDDLIMLKPQQVDAETGKAAEPAVFTTREILRIE
YDMSRSARVLSERRGFAVSHRVVAAAIESVETADPEKSFRLDPEQVDAVHHVTGDSGIAAVVGLAGAGKS
TLLAAARVAWESEGRRVIGAALAGKAAEGLEDSSGIRSRTLASWELIWANGHETLHRGDVVVVDEAGMVS
SQQMARVLKLVEQAGAKVVLVGDAMQLQPIQAGAAFRAITERIGFAELAGVRRQREDWAREASRLFARGE
IEKGLDAYAQQGHLVEAATRDEIIDRIVADWSEARKLAIETSVLEGNDGRLRGDELLVLAHTNADVKRLN
EALRSVMIGEGALGEGRSFRAERGAREFAAGDRIIFLENARFLEPRAPRLGPQYVKNGMLGTVVSTGDKR
GEVLLSVRLDNGRDVVISEDSYRNVDHGYAATIHKSQGATVERTFVLATGMMDQHLTYVSMTRHRDRADL
YAAKEDFEARPEWGRKPRVDHAAGVTGELVETGQAKFRPNDQDADDSPYADVKTDDGSVHRLWGVSLPKA
LEEGGVSEGDTVTLRKDGVEKVKVQVPIVDEQTGQKRFEEREVDRNVWTAKRIETAEARLERIEQESHRP
DLFKQLVERLSRAGAKTTTLDFESEAGYQAHADDFARRRGLDTLSRAAAGMEEEVSRRWAWIAEKREQVA
ELWERASMALGFAIERERRVSYNEERIETQPVSDADAGRARHLIPSTTVFARSVDEDARLAQLASPTWKE
REAILRPLLEKIYRDPDAALSALNALSSDTSIEPRKLAEDLAAAPDRLGRLRGSDLIVDGRAARDERNAA
ITALTELLPLARARATEFRRNAERFDLREQQRRAHMSLPIPALSKAAMARLAEIEAVRERGGDDAYKTAF
ALATEDRSLVQEVKAASDALSARFGWSAFTVKADAVAERNMVERMPEDLTSTRREKLARLFEAVKRFAEE
QHLVERRDRSKIVAAASADQGVETDKENAAVLPMLAAVTEFKTPVDEEARSRALATPLYRQQRAALAAVA
STIWRDPAGAVGKIEELLQKGFAAERIAAAVTNDPAAYGALRGSDRLKDRMLAAGRERKEAVQAVPEAAA
RLRALGSAYVNVLDATRQAVAEERRRMAVAIPGLSKAAEEALRRLTAEVKNNGRKRNATAVSLDPIIRQE
FAAVSRALDERFGRSAVIGGEKDLINRVPQAQHRVFEAMQDRLKVLQQTVRRESSEQIISERRQRAVSRG
HGVGL

Protein domains

Predicted by InterproScan.

• AAA domain (AAA_30) (391-577)

• MobA/MobL family (MobA_MobL) (17-249)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1047-1127)

• BID domain of Bartonella effector protein (Bep) (Bep_C_terminal) (1315-1406)

Protein structure

No available structure.

Host bacterium

• ID: 21673
• GenBank: NZ_CP088097
• Plasmid name: pSU303_2
• Incompatibility group: -
• Plasmid size: 529550 bp
• Coordinate of oriT [Strand]: 240404..240432 [+]
• Host baterium: Rhizobium sp. SU303

Cargo genes

• Drug resistance gene: -
• Virulence gene: -
• Metal resistance gene: actP
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA9