Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   121244
Name   oriT_pZN2-tetX-171kb in_silico
Organism   Proteus terrae subsp. cibarius strain ZN2
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP047350 (89200..89350 [+], 151 nt)
oriT length   151 nt
IRs (inverted repeats)      93..98, 106..111  (TGGCCT..AGGCCA)
 12..17, 24..29  (AACCCT..AGGGTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 151 nt

>oriT_pZN2-tetX-171kb
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   13415 GenBank   WP_001337757
Name   mobH_GTH25_RS18605_pZN2-tetX-171kb insolico UniProt ID   A0A709FRI2
Length   992 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 992 a.a.        Molecular weight: 109581.85 Da        Isoelectric Point: 4.8956

>WP_001337757.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEADDGDDQEEGEAALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGPTESSK
PDAGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPF
DAFNASAETTSTDATNSEIPDVAMPGKQEEQPKQDFVPQEQNSLQGDDFPMFGGSDEPPSWAIEPLPMLT
DAPEQPTHTPEMPHTDNVNQHEKDAKTLLVEMLSGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVI
LYPDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQ
DAFELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKASPEQKAKGKDSQPQPKEKKVDVTSPVEEQQ
RKPVQEKQNVARLPKREAQPVAPEPKVEREKELGHVEVREREDPEVREFEPPKAKTNPKDINAEDFLPSG
VTPQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKK
RQGKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure


Source ID Structure
AlphaFold DB A0A709FRI2


T4CP

ID   15719 GenBank   WP_000178856
Name   traD_GTH25_RS18610_pZN2-tetX-171kb insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69197.66 Da        Isoelectric Point: 6.9403

>WP_000178856.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPDDMWLGSGFLWENRHAQRVFEILKRDWTSIVGRESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVMPDWEAEAAAYLEKAKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-297)

  Protein structure



No available structure.



ID   15720 GenBank   WP_000637386
Name   traC_GTH25_RS18690_pZN2-tetX-171kb insolico UniProt ID   _
Length   815 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 815 a.a.        Molecular weight: 92590.87 Da        Isoelectric Point: 5.9222

>WP_000637386.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIVTIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSAGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTTLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTERIFAKTNKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQQLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSKGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRQQAA

  Protein domains


Predicted by InterproScan.

TraG P-loop domain (P-loop_TraG)

(449-809)

Family of unknown function (DUF5934) (DUF5934)

(284-434)

TraC protein (TraC_F_IV)

(21-255)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 87284..112475

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTH25_RS18595 (GTH25_18460) 83309..83542 - 234 WP_001191892 hypothetical protein -
GTH25_RS18600 (GTH25_18465) 83524..84141 - 618 WP_001249396 hypothetical protein -
GTH25_RS18605 (GTH25_18470) 84309..87287 + 2979 WP_001337757 MobH family relaxase -
GTH25_RS18610 (GTH25_18475) 87284..89149 + 1866 WP_000178856 conjugative transfer system coupling protein TraD virb4
GTH25_RS18615 (GTH25_18480) 89199..89744 + 546 WP_000228720 hypothetical protein -
GTH25_RS18620 (GTH25_18485) 89701..90330 + 630 WP_000743450 DUF4400 domain-containing protein tfc7
GTH25_RS19145 90378..90554 + 177 WP_169342285 hypothetical protein -
GTH25_RS18630 (GTH25_18495) 90796..91173 + 378 WP_000869261 hypothetical protein -
GTH25_RS18635 (GTH25_18500) 91173..91835 + 663 WP_001231463 hypothetical protein -
GTH25_RS18640 92016..92159 + 144 WP_001275801 hypothetical protein -
GTH25_RS18645 (GTH25_18505) 92171..92536 + 366 WP_001052531 hypothetical protein -
GTH25_RS18650 (GTH25_18510) 92681..92962 + 282 WP_000805625 type IV conjugative transfer system protein TraL traL
GTH25_RS18655 (GTH25_18515) 92959..93585 + 627 WP_001049716 TraE/TraK family type IV conjugative transfer system protein traE
GTH25_RS18660 (GTH25_18520) 93569..94486 + 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
GTH25_RS18665 (GTH25_18525) 94486..95799 + 1314 WP_024131605 TraB/VirB10 family protein traB
GTH25_RS18670 (GTH25_18530) 95796..96374 + 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
GTH25_RS18675 (GTH25_18535) 96378..96770 + 393 WP_000479535 TraA family conjugative transfer protein -
GTH25_RS18680 (GTH25_18540) 96971..102502 + 5532 WP_000606833 Ig-like domain-containing protein -
GTH25_RS18685 (GTH25_18545) 102651..103358 + 708 WP_001259347 DsbC family protein -
GTH25_RS18690 (GTH25_18550) 103355..105802 + 2448 WP_000637386 type IV secretion system protein TraC virb4
GTH25_RS18695 (GTH25_18555) 105817..106134 + 318 WP_000351984 hypothetical protein -
GTH25_RS18700 (GTH25_18560) 106131..106661 + 531 WP_001010738 S26 family signal peptidase -
GTH25_RS18705 (GTH25_18565) 106624..107889 + 1266 WP_000621288 TrbC family F-type conjugative pilus assembly protein traW
GTH25_RS18710 (GTH25_18570) 107886..108557 + 672 WP_001337754 EAL domain-containing protein -
GTH25_RS18715 (GTH25_18575) 108557..109561 + 1005 WP_043940352 TraU family protein traU
GTH25_RS18720 (GTH25_18580) 109677..112475 + 2799 WP_001256487 conjugal transfer mating pair stabilization protein TraN traN
GTH25_RS18725 (GTH25_18585) 112514..113374 - 861 WP_000709517 hypothetical protein -
GTH25_RS18730 (GTH25_18590) 113497..114138 - 642 WP_000796664 hypothetical protein -
GTH25_RS18735 (GTH25_18595) 114432..114752 + 321 WP_000547566 hypothetical protein -
GTH25_RS18740 (GTH25_18600) 115056..115241 + 186 WP_001186917 hypothetical protein -
GTH25_RS18745 (GTH25_18605) 115461..116429 + 969 WP_000085162 AAA family ATPase -
GTH25_RS18750 (GTH25_18610) 116440..117348 + 909 WP_000739139 hypothetical protein -

Host bacterium

ID   21672 GenBank   NZ_CP047350
Plasmid name   pZN2-tetX-171kb Incompatibility group   IncA/C2
Plasmid size   171208 bp Coordinate of oriT [Strand]   89200..89350 [+]
Host baterium   Proteus terrae subsp. cibarius strain ZN2

Cargo genes

Drug resistance gene   aph(3')-Ia, aac(3)-IId, blaTEM-1B, aph(6)-Id, aph(3'')-Ib, tet(X6), floR, sul2
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -