Detailed information of oriT

oriT

The information of the oriT region

• oriTDB ID: 121218
• Name: oriT_R17|unnamed
• Organism: Enterococcus hirae strain R17
• Sequence Completeness: -
• NCBI accession of oriT (coordinates [strand]): NZ_CP015517 (28228..28265 [+], 38 nt)
• oriT length: 38 nt
• IRs (inverted repeats): 14..22, 29..37 (TAAAGTATA..TATACTTTA) 2..8, 13..19 (ACTTTAT..ATAAAGT)
• Location of nic site: 27..28
• Conserved sequence flanking the nic site: GTGTGTTATA
• Note: Predicted by oriTfinder 2.0

oriT sequence

• Length: 38 nt

>oriT_R17|unnamed
CACTTTATGAATATAAAGTATAGTGTGTTATACTTTAC

oriT secondary structure

Predicted by RNAfold.

Relaxase

mobP2_A6P53_RS13120_R17|unnamed

• ID: 13401
• GenBank: WP_063627755
• Name: mobP2_A6P53_RS13120_R17|unnamed
• UniProt ID: _
• Length: 530 a.a.
• Note: Predicted by oriTfinder 2.0

Relaxase protein sequence

• Length: 530 a.a.
• Molecular weight: 63012.19 Da
• Isoelectric Point: 9.3856

>WP_063627755.1 MULTISPECIES: MobP2 family relaxase [Enterococcus]
MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKSLEEMERRTPEEEQELQRIKNALEDFYIPEGETFSESK
KDKEFSETANEAQSLMGNGINFDKLEEQNFTKYLSYMTRHYALEQKKELTQSEQKEYQRLNQAIKKYQTE
PHKKASGNLPGVFSMASDEVKGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEE
GLKAAGRKMMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKGKRRQ
ATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYDRHAMNLLNEVYKTLPD
KRSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQISKALKEEAKSLYGESKRESKDAEKNAL
FDLKKRTGNAILTELKKQDSQIKPLIESMDQMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRV
VTQRSLRQLNRTINAHEEKYHASKAYEEQQRRIQMEQERQ

Protein domains

Predicted by InterproScan.

• MobL relaxases (MobL) (4-442)

Protein structure

No available structure.

T4CP

t4cp2_A6P53_RS13185_R17|unnamed

• ID: 15707
• GenBank: WP_063627764
• Name: t4cp2_A6P53_RS13185_R17|unnamed
• UniProt ID: _
• Length: 871 a.a.
• PDB ID: _
• Note: Predicted by oriTfinder 2.0

T4CP protein sequence

• Length: 871 a.a.
• Molecular weight: 100557.60 Da
• Isoelectric Point: 6.2821

>WP_063627764.1 MULTISPECIES: VirD4-like conjugal transfer protein, CD1115 family [Enterococcus]
MRVRETTKRKNKPFNLEKKKYLSHFYRYAPAGERLPGKHDKRVKQKLMYSGLVYLPLDYLVTRIPEAFSR
FEIIQGSLQGMNLFQLQEMPQIFHFDSAGFWNPLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLAD
GQKGDNRLMTLKEIQQIYPQIPDTKGSFPGYGGIPITHYKKYWYIQTDTVNTCIIGTSRSGKGQIEVLAT
IDNLSRAEKQSSLVVNDPKNELYVASKDELEARGYDVYAYNLTEPLQSMSDNPLALIVKYWQRGDVDTAT
QLANTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNNLVELLSEIGGFNYYPD
PENEFKQLNALDEFMNQLPPGNLAKKQYGATKIGSDKAKGNILSTAITGLNPFTLTKIAKMTSQSTIEYK
SVGFPKYLDLKLHESLLNQRISIEFYHEDKKFHEERVKVGYKGFCEINFDCHLEQGDQVCLKYDHKGKEY
VAWYEFSQKVLKDEKGKIVYRKKRGETHLPEWEKEAVLKLDESKSNLLVESIRMHYTDKPMAIFMLTPDF
DPSNHIIVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNMLWDLFIQ
SYKQLRAKYGEDNADIIKENCQTHIYIMSSNTDTIEEFSQNVGNKTVEQENSTINALGMNTHINRNVDSD
RILPPERIRSLLKGETIVLAPLKREDLKGNKIRPYPIFNSEKTNSPYAYQYLEDFDTLGRTIHDIDIYCT
HADLDLKALQIDYDYFLPDEDARDLYHGLHEQLEEKIEDNMFYKIVQSLENETAKKLLIQFYEEQATDRL
QEYVEKLIKNQEISEMIGKRLLLEADKAVKE

Protein domains

Predicted by InterproScan.

• Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf) (188-419)

• TraM recognition site of TraD and TraG (TraG-D_C) (594-711)

Protein structure

No available structure.

Host bacterium

• ID: 21646
• GenBank: NZ_CP015517
• Plasmid name: R17|unnamed
• Incompatibility group: -
• Plasmid size: 73574 bp
• Coordinate of oriT [Strand]: 28228..28265 [+]
• Host baterium: Enterococcus hirae strain R17

Cargo genes

• Drug resistance gene: tet(M), tet(L), erm(B)
• Virulence gene: -
• Metal resistance gene: -
• Degradation gene: -
• Symbiosis gene: -
• Anti-CRISPR: AcrIIA21