Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 121174 |
| Name | oriT_plca36  |
| Organism | Lacticaseibacillus paracasei |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NC_011352 (3222..3259 [-], 38 nt) |
| oriT length | 38 nt |
| IRs (inverted repeats) | 1..7, 19..25 (ACACAAC..GTTGTGT) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 38 nt
>oriT_plca36
ACACAACCAACTTTAGTTGTTGTGTGTAAGTGCGCATT
ACACAACCAACTTTAGTTGTTGTGTGTAAGTGCGCATT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 13368 | GenBank | WP_043925530 |
| Name | mobQ_LCAZH_RS00035_plca36 |
UniProt ID | _ |
| Length | 687 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 687 a.a. Molecular weight: 80720.36 Da Isoelectric Point: 9.6432
>WP_043925530.1 MobQ family relaxase [Lacticaseibacillus paracasei]
MAIFHMSFSNISAGKGRSAIASAAYRSGKKLFDKKEGRSYFYARSVMPESFILTPKNAPEWASDREQLWN
EVERKDRRANSRYAKEFNVALPVELSEDEQKELLTKYVQENFVDEGMVADVAIHRDHPDNPHAHVMLTNR
PFNPDGTWGLKSKRENILDENGNKTYTGNSRFPRSRKVWLVDWDKKEKINQWRHNWAVSVNQVLEQKNIP
DRISEKSFIEQGIDDTPMQHEGINSKRYERKEFNQQVKNYRKAKASYKNNQEKVINRGHLDSLSRHFSFN
EKRVVKELSHELKTYISLESLDDKRRMLFNWKNSTLIKHAVGEDVTKQLLTINQQESSLKKADELLNKVV
DRTTKKLYPELNFEQTTQAERRELIKETDSEQTIFKGSELNERLMNIRDDLLTQQLLTFTKRPYVGWKLL
MQQEKEVKIELKYTLMIHDDNLESLEHVDQGLLEKYSPTEQQKITRAVKDLRAIMAVKQVIKTQYHEVLK
RAFPKGDLNGLPLIKQEQAYTAVMYYDPVLKPCQAETIAQWQENPPRVFNTQEHLQGLAYLSGQLSLDQL
ENHHLQRVLKHDGTRQLFLGECKVDPTIKNSQIEKIQKQLKGQQAKDDQYRKANIGHYQPLNYRPVSPSY
YLKTAFSNAIMTALYAHDEDYERQKQARSLKETEWEMTKKQRQHQTRNRHEDGGMHL
MAIFHMSFSNISAGKGRSAIASAAYRSGKKLFDKKEGRSYFYARSVMPESFILTPKNAPEWASDREQLWN
EVERKDRRANSRYAKEFNVALPVELSEDEQKELLTKYVQENFVDEGMVADVAIHRDHPDNPHAHVMLTNR
PFNPDGTWGLKSKRENILDENGNKTYTGNSRFPRSRKVWLVDWDKKEKINQWRHNWAVSVNQVLEQKNIP
DRISEKSFIEQGIDDTPMQHEGINSKRYERKEFNQQVKNYRKAKASYKNNQEKVINRGHLDSLSRHFSFN
EKRVVKELSHELKTYISLESLDDKRRMLFNWKNSTLIKHAVGEDVTKQLLTINQQESSLKKADELLNKVV
DRTTKKLYPELNFEQTTQAERRELIKETDSEQTIFKGSELNERLMNIRDDLLTQQLLTFTKRPYVGWKLL
MQQEKEVKIELKYTLMIHDDNLESLEHVDQGLLEKYSPTEQQKITRAVKDLRAIMAVKQVIKTQYHEVLK
RAFPKGDLNGLPLIKQEQAYTAVMYYDPVLKPCQAETIAQWQENPPRVFNTQEHLQGLAYLSGQLSLDQL
ENHHLQRVLKHDGTRQLFLGECKVDPTIKNSQIEKIQKQLKGQQAKDDQYRKANIGHYQPLNYRPVSPSY
YLKTAFSNAIMTALYAHDEDYERQKQARSLKETEWEMTKKQRQHQTRNRHEDGGMHL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 15667 | GenBank | WP_012552819 |
| Name | t4cp2_LCAZH_RS00095_plca36 |
UniProt ID | _ |
| Length | 514 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 514 a.a. Molecular weight: 57737.01 Da Isoelectric Point: 5.1180
>WP_012552819.1 VirD4-like conjugal transfer protein, CD1115 family [Lacticaseibacillus paracasei]
MNGTILGIVDKQIVYQNNSTKPNRNIFVVGGPGSYKTQSVVITNLFNETENSIVVTDPKGELYEKAAGIK
LAQGYQVHVVNFANMAHSDRYNPFDYIQRDIQAETVATKIVQSENAEGKKDVWFSTQRQLLKALILFVMN
HRSPEQRNLAGVTQVLQENDVEAEEKGADSPLDTLFLELTMTDPARRAYELGFKKAKGEMKASIIESLLA
TISKFVDKEVADFTSFSDFDLKEIGKAKVVLYVIIPVMDNTYESFINLFFSQLFDELYKLAADHHAKLPH
QVDFILDEFVNLGKFPKYEEFLATCRGYGIGVTTICQTLTQLQALYGKEKAESILGNHAVKICLNAANDV
TAKYFSDLLGKSTVKVETGSESTSHSKEESHSKSDSYSYTSRSLMTPDEIMRMPEDQSLLIFSNARPVKA
TKAFQFKLFPGADHLVNLSQNEYQGQPEASQETNFHQRVAKWKAKLKETAQKKAANKMSQTEEEQQQDSL
DSDLERVSNKDSQTEEPDDDNLLG
MNGTILGIVDKQIVYQNNSTKPNRNIFVVGGPGSYKTQSVVITNLFNETENSIVVTDPKGELYEKAAGIK
LAQGYQVHVVNFANMAHSDRYNPFDYIQRDIQAETVATKIVQSENAEGKKDVWFSTQRQLLKALILFVMN
HRSPEQRNLAGVTQVLQENDVEAEEKGADSPLDTLFLELTMTDPARRAYELGFKKAKGEMKASIIESLLA
TISKFVDKEVADFTSFSDFDLKEIGKAKVVLYVIIPVMDNTYESFINLFFSQLFDELYKLAADHHAKLPH
QVDFILDEFVNLGKFPKYEEFLATCRGYGIGVTTICQTLTQLQALYGKEKAESILGNHAVKICLNAANDV
TAKYFSDLLGKSTVKVETGSESTSHSKEESHSKSDSYSYTSRSLMTPDEIMRMPEDQSLLIFSNARPVKA
TKAFQFKLFPGADHLVNLSQNEYQGQPEASQETNFHQRVAKWKAKLKETAQKKAANKMSQTEEEQQQDSL
DSDLERVSNKDSQTEEPDDDNLLG
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 6811..16893
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LCAZH_RS00020 (LCAZH_p004) | 1892..2572 | + | 681 | WP_012552805 | zeta toxin family protein | - |
| LCAZH_RS00025 (LCAZH_p005) | 2562..2840 | - | 279 | WP_012552806 | hypothetical protein | - |
| LCAZH_RS00030 (LCAZH_p006) | 2863..3090 | - | 228 | WP_012552807 | hypothetical protein | - |
| LCAZH_RS00035 (LCAZH_p007) | 3344..5407 | + | 2064 | WP_043925530 | MobQ family relaxase | - |
| LCAZH_RS15300 | 5491..5802 | + | 312 | WP_003712158 | hypothetical protein | - |
| LCAZH_RS00045 (LCAZH_p008) | 5839..6453 | + | 615 | WP_012552809 | hypothetical protein | - |
| LCAZH_RS00050 (LCAZH_p009) | 6455..6790 | + | 336 | WP_012552810 | CagC family type IV secretion system protein | - |
| LCAZH_RS00055 (LCAZH_p010) | 6811..7173 | + | 363 | WP_003590770 | conjugal transfer protein | trsC |
| LCAZH_RS00060 (LCAZH_p011) | 7142..7801 | + | 660 | WP_012552811 | TrsD/TraD family conjugative transfer protein | trsD |
| LCAZH_RS00065 (LCAZH_p012) | 7813..9831 | + | 2019 | WP_012552812 | VirB4 family type IV secretion system protein | virb4 |
| LCAZH_RS00070 (LCAZH_p013) | 9824..11242 | + | 1419 | WP_012552813 | conjugal transfer protein | trsF |
| LCAZH_RS00075 (LCAZH_p014) | 11243..12400 | + | 1158 | WP_012552814 | phage tail tip lysozyme | prgK |
| LCAZH_RS00080 (LCAZH_p015) | 12414..13031 | + | 618 | WP_012552815 | hypothetical protein | - |
| LCAZH_RS00085 (LCAZH_p016) | 13018..13386 | + | 369 | WP_012552816 | thioredoxin family protein | - |
| LCAZH_RS00090 (LCAZH_p017) | 13387..13857 | + | 471 | WP_012552817 | hypothetical protein | trsJ |
| LCAZH_RS00095 (LCAZH_p018) | 14087..15631 | + | 1545 | WP_012552819 | VirD4-like conjugal transfer protein, CD1115 family | - |
| LCAZH_RS00100 (LCAZH_p019) | 15646..16035 | + | 390 | WP_003712166 | hypothetical protein | - |
| LCAZH_RS00105 (LCAZH_p020) | 16054..16893 | + | 840 | WP_012552820 | conjugal transfer protein TrbL family protein | trsL |
| LCAZH_RS00110 (LCAZH_p021) | 16908..17318 | + | 411 | WP_012552821 | hypothetical protein | - |
| LCAZH_RS00115 (LCAZH_p022) | 17325..19460 | + | 2136 | WP_012552822 | type IA DNA topoisomerase | - |
| LCAZH_RS00120 (LCAZH_p023) | 19583..19798 | + | 216 | WP_012552823 | hypothetical protein | - |
| LCAZH_RS14690 (LCAZH_p024) | 19802..20926 | + | 1125 | WP_012552824 | hypothetical protein | - |
| LCAZH_RS14695 | 21080..21169 | - | 90 | WP_021353390 | putative holin-like toxin | - |
Host bacterium
| ID | 21602 | GenBank | NC_011352 |
| Plasmid name | plca36 | Incompatibility group | - |
| Plasmid size | 36487 bp | Coordinate of oriT [Strand] | 3222..3259 [-] |
| Host baterium | Lacticaseibacillus paracasei |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA21 |