Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   121164
Name   oriT_LB UEL H-11|p1 in_silico
Organism   Proteus mirabilis strain LB UEL H-11
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP086376 (38233..38383 [+], 151 nt)
oriT length   151 nt
IRs (inverted repeats)      93..98, 106..111  (TGGCCT..AGGCCA)
 12..17, 24..29  (AACCCT..AGGGTT)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 151 nt

>oriT_LB UEL H-11|p1
TGGTCGATGTGAACCCTTTCGACAGGGTTATGAATGAATTGAAAAGTCGTGGCCGCAAGAACGCTCACATCCTGAGCATCCTCCAATTCGACTGGCCTGCATCGGAGGCCATCATCGAGAAGCTGAGCTGCTACATCACAGACGGGATTAA

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   13360 GenBank   WP_001337757
Name   mobH_LK398_RS00225_LB UEL H-11|p1 insolico UniProt ID   A0A709FRI2
Length   992 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 992 a.a.        Molecular weight: 109581.85 Da        Isoelectric Point: 4.8956

>WP_001337757.1 MULTISPECIES: MobH family relaxase [Gammaproteobacteria]
MLKALNKLFGGRSGVIETAPSARVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAELIEKVRNSLGF
TVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQASESVIFSIEGTPRERRDNE
PRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSESLHDWAHRHEIDRYFIRWRDKRHKRHEQFSL
LAVDRIIPAETREFLSKSGPSIMEAMLEAISGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVP
VERYVFDAIRRLVKTGKWKVNEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIER
GFAVPNTVQEKGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE
FVDPEEADDGDDQEEGEAALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKDAGPTESSK
PDAGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNPLQALKDVGGGLGDIDFPF
DAFNASAETTSTDATNSEIPDVAMPGKQEEQPKQDFVPQEQNSLQGDDFPMFGGSDEPPSWAIEPLPMLT
DAPEQPTHTPEMPHTDNVNQHEKDAKTLLVEMLSGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVI
LYPDGARSLGAPSEVLSKLSHANAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQ
DAFELVSPPGLDASKNKSAPKQQSRKKAQQQKPEVNAGKASPEQKAKGKDSQPQPKEKKVDVTSPVEEQQ
RKPVQEKQNVARLPKREAQPVAPEPKVEREKELGHVEVREREDPEVREFEPPKAKTNPKDINAEDFLPSG
VTPQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISKHILCGMLSRAQRRPLLKK
RQGKLYLEVNET

  Protein domains


Predicted by InterproScan.

Putative helicase (TraI_2)

(51-357)


  Protein structure


Source ID Structure
AlphaFold DB A0A709FRI2


T4CP

ID   15653 GenBank   WP_000178856
Name   traD_LK398_RS00230_LB UEL H-11|p1 insolico UniProt ID   _
Length   621 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 621 a.a.        Molecular weight: 69197.66 Da        Isoelectric Point: 6.9403

>WP_000178856.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Gammaproteobacteria]
MTMSYDPLAYEMPWRPNYEKNAVAGWLAASGAALAVEQVSTMPPEPFYWMTGICGVMAMARLPKAIKLHL
LQKHLKGRDLEFISIAELQKYIKDTPDDMWLGSGFLWENRHAQRVFEILKRDWTSIVGRESTVKKVVRKI
QGKKKELPIGQPWIHGVEPKEEKLMQPLKHTEGHSLIVGTTGSGKTRMFDILISQAILRGEAVIIIDPKG
DKEMRDNARRACEAMGQPERFVSFHPAFPEESVRIDPLRNFTRVTEIASRLAALIPSEAGADPFKSFGWQ
ALNNIAQGLVITHDRPNLTKLRRFLEGGAAGLVIKAVQAYSERVMPDWEAEAAAYLEKAKNGSREKIAFA
LMKFYYDIIQPEHPNSDLEGLLSMFQHDQTHFSKMVANLLPIMNMLTSGELGPLLSPDSSDLSDERQITD
SAKIINNAQVAYLGLDSLTDNMVGSAMGSIFLSDLTAVAGDRYNYGVNNRPVNIFVDEAAEVINDPFIQL
LNKGRGAKLRLFVATQTFADFAARLGSKDKALQVLGNINNTFALRIVDGETQEYIADNLPKTRLKYVMRT
QGQNSDGKEPIMHGGNQGERLMEEEADLFPAQLLGMLPNLEYIAKISGGTIVKGRLPILTQ

  Protein domains


Predicted by InterproScan.

TraM recognition site of TraD and TraG (TraG-D_C)

(471-598)

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(170-297)

  Protein structure



No available structure.



ID   15654 GenBank   WP_000637386
Name   traC_LK398_RS00310_LB UEL H-11|p1 insolico UniProt ID   _
Length   815 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 815 a.a.        Molecular weight: 92590.87 Da        Isoelectric Point: 5.9222

>WP_000637386.1 MULTISPECIES: type IV secretion system protein TraC [Gammaproteobacteria]
MIVTIKKKLEETLIPEHLRAAGIIPVLAYDEDDHVFLMDDHSAGFGFMCEPLCGADEKVQERMNGFLNQE
FPSKTTLQFVLFRSPDINQEMYRMMGLRDGFRHELLTSVIKERINFLQHHTTERIFAKTNKGIYDNGLIQ
DLKLFVTCKVPIKNNNPTESELQQLAQLRTKVESSLQTVGLRPRTMTAVNYIRIMSTILNWGPDASWRHD
SVDWEMDKPICEQIFDYGTDVEVSKNGIRLGDYHAKVMSAKKLPDVFYFGDALTYAGDLSGGNSSIKENY
MVVTNVFFPEAESTKNTLERKRQFTVNQAYGPMLKFVPVLADKKESFDTLYESMKEGAKPVKITYSVVLF
APTKERVEAAAMAARNIWRESRFELMEDKFVALPMFLNCLPFCTDRDAVRDLFRYKTMTTEQAAVVLPVF
GEWKGTGTYHAALISRNGQLMSLSLHDSNTNKNLVIAAESGSGKSFLTNELIFSYLSEGAQVWVIDAGKS
YQKLSEMLNGDFVHFEEGTHVCLNPFELIQNYEDEEDAIVSLVCAMASAKGLLDEWQISALKQVLSRLWE
EKGKEMKVDDIAERCLEEENDQRLKDIGQQLYAFTSKGSYGKYFSRKNNVSFQNQFTVLELDELQGRKHL
RQVVLLQLIYQIQQEVFLGERNRKKVVIVDEAWDLLKEGEVSVFMEHAYRKFRKYGGSVVIATQSINDLY
ENAVGRAIAENSASMYLLGQTEETVESVKRSGRLTLSEGGFHTLKTVHTIQGVYSEIFIKSKSGMGVGRL
IVGDFQKLLYSTDPVDVNAIDQFVKQGMSIPEAIKAVMRSRQQAA

  Protein domains


Predicted by InterproScan.

TraG P-loop domain (P-loop_TraG)

(449-809)

Family of unknown function (DUF5934) (DUF5934)

(284-434)

TraC protein (TraC_F_IV)

(21-255)

  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 36317..61508

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LK398_RS00215 (LK398_00215) 32342..32575 - 234 WP_001191892 hypothetical protein -
LK398_RS00220 (LK398_00220) 32557..33174 - 618 WP_001249396 hypothetical protein -
LK398_RS00225 (LK398_00225) 33342..36320 + 2979 WP_001337757 MobH family relaxase -
LK398_RS00230 (LK398_00230) 36317..38182 + 1866 WP_000178856 conjugative transfer system coupling protein TraD virb4
LK398_RS00235 (LK398_00235) 38232..38777 + 546 WP_000228720 hypothetical protein -
LK398_RS00240 (LK398_00240) 38734..39363 + 630 WP_000743450 DUF4400 domain-containing protein tfc7
LK398_RS00245 (LK398_00245) 39411..39587 + 177 WP_169342285 hypothetical protein -
LK398_RS00250 (LK398_00250) 39829..40206 + 378 WP_000869261 hypothetical protein -
LK398_RS00255 (LK398_00255) 40206..40868 + 663 WP_001231463 hypothetical protein -
LK398_RS00260 (LK398_00260) 41049..41192 + 144 WP_001275801 hypothetical protein -
LK398_RS00265 (LK398_00265) 41204..41569 + 366 WP_001052531 hypothetical protein -
LK398_RS00270 (LK398_00270) 41714..41995 + 282 WP_000805625 type IV conjugative transfer system protein TraL traL
LK398_RS00275 (LK398_00275) 41992..42618 + 627 WP_001049716 TraE/TraK family type IV conjugative transfer system protein traE
LK398_RS00280 (LK398_00280) 42602..43519 + 918 WP_000794249 type-F conjugative transfer system secretin TraK traK
LK398_RS00285 (LK398_00285) 43519..44832 + 1314 WP_024131605 TraB/VirB10 family protein traB
LK398_RS00290 (LK398_00290) 44829..45407 + 579 WP_000793435 type IV conjugative transfer system lipoprotein TraV traV
LK398_RS00295 (LK398_00295) 45411..45803 + 393 WP_000479535 TraA family conjugative transfer protein -
LK398_RS00300 (LK398_00300) 46004..51535 + 5532 WP_109829093 Ig-like domain-containing protein -
LK398_RS00305 (LK398_00305) 51684..52391 + 708 WP_001259347 DsbC family protein -
LK398_RS00310 (LK398_00310) 52388..54835 + 2448 WP_000637386 type IV secretion system protein TraC virb4
LK398_RS00315 (LK398_00315) 54850..55167 + 318 WP_000351984 hypothetical protein -
LK398_RS00320 (LK398_00320) 55164..55694 + 531 WP_001010738 S26 family signal peptidase -
LK398_RS00325 (LK398_00325) 55657..56922 + 1266 WP_000621288 TrbC family F-type conjugative pilus assembly protein traW
LK398_RS00330 (LK398_00330) 56919..57590 + 672 WP_001337754 EAL domain-containing protein -
LK398_RS00335 (LK398_00335) 57590..58594 + 1005 WP_043940352 TraU family protein traU
LK398_RS00340 (LK398_00340) 58710..61508 + 2799 WP_001256487 conjugal transfer mating pair stabilization protein TraN traN
LK398_RS00345 (LK398_00345) 61547..62407 - 861 WP_000709517 hypothetical protein -
LK398_RS00350 (LK398_00350) 62530..63171 - 642 WP_000796664 hypothetical protein -
LK398_RS00355 (LK398_00355) 63465..63785 + 321 WP_000547566 hypothetical protein -
LK398_RS00360 (LK398_00360) 64089..64274 + 186 WP_001186917 hypothetical protein -
LK398_RS00365 (LK398_00365) 64494..65462 + 969 WP_000085162 AAA family ATPase -
LK398_RS00370 (LK398_00370) 65473..66381 + 909 WP_000739139 hypothetical protein -

Host bacterium

ID   21592 GenBank   NZ_CP086376
Plasmid name   LB UEL H-11|p1 Incompatibility group   IncA/C2
Plasmid size   160563 bp Coordinate of oriT [Strand]   38233..38383 [+]
Host baterium   Proteus mirabilis strain LB UEL H-11

Cargo genes

Drug resistance gene   tet(G), floR, qacE, aadA2, dfrA12, aac(3)-IId, blaTEM-1B, aph(6)-Id, aph(3'')-Ib, sul2, blaCTX-M-14
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -