Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   121162
Name   oriT_pKBSW16i-B in_silico
Organism   Escherichia albertii strain KBSW16i
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP110262 (33690..33778 [-], 89 nt)
oriT length   89 nt
IRs (inverted repeats)     _
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 89 nt

>oriT_pKBSW16i-B
GGGGTGTCGGGGCGAAGCCCTGACCAGATGGTAATTGTAATAGCGTCGCGTGTGACGGTATTACAATTACACATCCTGTCCCATTTTTC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   13359 GenBank   WP_001405892
Name   nikB_N6N74_RS24125_pKBSW16i-B insolico UniProt ID   A0A3V4WX06
Length   899 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 899 a.a.        Molecular weight: 103883.32 Da        Isoelectric Point: 7.3373

>WP_001405892.1 MULTISPECIES: IncI1-type relaxase NikB [Enterobacteriaceae]
MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRLRNESFVALVD
VMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIAQQAHYAKDNTDPVFHYILSWQAHESPRPEQIYDSVR
HTLKSLGLGEHQYVSAVHTDTDNLHVHVAVNRVHPVTGYLNCLSWSQEKLSRACRELELKHGFAPDNGCW
VHAPGNRIVRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGK
FLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETE
SLQQYACRHLGERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT
LDNVNQLDGGWQPVPTDIFRQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGY
SISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEP
QAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAWREQWRK
PDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLKEDIRDERQKLIADGKWYP
PSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDRSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGS
VRFRDRRTGEFVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW
HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(62-314)


  Protein structure


Source ID Structure
AlphaFold DB A0A3V4WX06


Auxiliary protein

ID   7388 GenBank   WP_001283947
Name   WP_001283947_pKBSW16i-B insolico UniProt ID   A0A142CMC2
Length   110 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 110 a.a.        Molecular weight: 12613.57 Da        Isoelectric Point: 10.7463

>WP_001283947.1 MULTISPECIES: IncI1-type relaxosome accessory protein NikA [Enterobacteriaceae]
MSDSAVRKKSEVRQKTVVRTLRFSPVEDETIRKKAEDSGLTVSAYIRNAALNKRINSRTDDAFLKELMRL
GRMQKHLFVQGKRTGDKEYAEVLVAITELTNTLRKQLMEG

  Protein domains



No domain identified.



  Protein structure


Source ID Structure
AlphaFold DB A0A142CMC2


T4CP

ID   15650 GenBank   WP_001289276
Name   trbC_N6N74_RS24130_pKBSW16i-B insolico UniProt ID   _
Length   763 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 763 a.a.        Molecular weight: 86941.11 Da        Isoelectric Point: 6.7713

>WP_001289276.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIWPLALPACLLFTLITMLVFSLQRWRCP
LRMPMTLECADPSQDRMIKRSLFSFWPTLFQYEVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIM
FFATTGGGKTETIFAWAINPLCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEI
ILSGEKTRPQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGKTMSL
QMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDLSLVRTPSAWTEEPRKQHAYLSGQFSE
TFSTFTEAFGDIFAEDSGDIDIRDSIHSDRILMVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLE
GTDSDALEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTATNTATLM
QNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQRQDGIFGESWIDSPQISILMESKINVQELIELH
PGEFFSIFRGETVPSASFFIPDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAII
GVLTAKPSRKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTREERR
IRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKTNRPDWDGMY

  Protein domains



No domain identified.


  Protein structure



No available structure.




T4SS

T4SS were predicted by using oriTfinder2.

Region 1: 39157..79115

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N6N74_RS24130 (N6N74_24155) 36873..39164 - 2292 WP_001289276 F-type conjugative transfer protein TrbC -
N6N74_RS24135 (N6N74_24160) 39157..40227 - 1071 WP_000151583 IncI1-type conjugal transfer protein TrbB trbB
N6N74_RS24140 (N6N74_24165) 40246..41454 - 1209 WP_016245570 IncI1-type conjugal transfer protein TrbA trbA
N6N74_RS24145 (N6N74_24170) 41746..41898 + 153 WP_001331364 Hok/Gef family protein -
N6N74_RS24150 (N6N74_24175) 41970..42221 - 252 WP_001291964 hypothetical protein -
N6N74_RS24570 42720..42815 + 96 WP_001303310 DinQ-like type I toxin DqlB -
N6N74_RS24160 (N6N74_24185) 42880..43017 - 138 WP_262930367 hypothetical protein -
N6N74_RS24165 (N6N74_24190) 43062..44288 - 1227 WP_024186316 RNA-guided endonuclease TnpB family protein -
N6N74_RS24170 (N6N74_24195) 44347..44751 + 405 WP_001175009 IS200/IS605 family transposase -
N6N74_RS24175 (N6N74_24200) 45083..45292 - 210 WP_001140543 hemolysin expression modulator Hha -
N6N74_RS24180 (N6N74_24205) 45390..46052 - 663 WP_000644794 plasmid IncI1-type surface exclusion protein ExcA -
N6N74_RS24185 (N6N74_24210) 46123..48291 - 2169 WP_000698368 DotA/TraY family protein traY
N6N74_RS24190 (N6N74_24215) 48388..48972 - 585 WP_001037985 IncI1-type conjugal transfer protein TraX -
N6N74_RS24195 (N6N74_24220) 49001..50203 - 1203 WP_001189156 IncI1-type conjugal transfer protein TraW traW
N6N74_RS24200 (N6N74_24225) 50170..50784 - 615 WP_000337399 IncI1-type conjugal transfer protein TraV traV
N6N74_RS24205 (N6N74_24230) 50784..53828 - 3045 WP_052980399 IncI1-type conjugal transfer protein TraU traU
N6N74_RS24210 (N6N74_24235) 53918..54718 - 801 WP_001164788 IncI1-type conjugal transfer protein TraT traT
N6N74_RS24215 (N6N74_24240) 54702..54890 - 189 WP_001277255 putative conjugal transfer protein TraS -
N6N74_RS24220 (N6N74_24245) 54954..55358 - 405 WP_000086960 IncI1-type conjugal transfer protein TraR traR
N6N74_RS24225 (N6N74_24250) 55409..55936 - 528 WP_001055569 conjugal transfer protein TraQ traQ
N6N74_RS24230 (N6N74_24255) 55936..56640 - 705 WP_000801920 IncI1-type conjugal transfer protein TraP traP
N6N74_RS24235 (N6N74_24260) 56640..57929 - 1290 WP_262930369 conjugal transfer protein TraO traO
N6N74_RS24240 (N6N74_24265) 57932..58915 - 984 WP_001191879 IncI1-type conjugal transfer protein TraN traN
N6N74_RS24245 (N6N74_24270) 58926..59618 - 693 WP_000138552 DotI/IcmL family type IV secretion protein traM
N6N74_RS24250 (N6N74_24275) 59615..59962 - 348 WP_001055900 conjugal transfer protein traL
N6N74_RS24255 (N6N74_24280) 59980..63747 - 3768 WP_001141537 LPD7 domain-containing protein -
N6N74_RS24260 (N6N74_24285) 63837..64388 - 552 WP_000014584 phospholipase D family protein -
N6N74_RS24265 (N6N74_24290) 64403..64693 - 291 WP_001299214 hypothetical protein traK
N6N74_RS24270 (N6N74_24295) 64690..65838 - 1149 WP_001024974 plasmid transfer ATPase TraJ virB11
N6N74_RS24275 (N6N74_24300) 65835..66653 - 819 WP_000646095 IncI1-type conjugal transfer lipoprotein TraI traI
N6N74_RS24280 (N6N74_24305) 66650..67108 - 459 WP_001079808 IncI1-type conjugal transfer lipoprotein TraH -
N6N74_RS24285 (N6N74_24310) 67503..68087 - 585 WP_000977522 histidine phosphatase family protein -
N6N74_RS24290 (N6N74_24315) 68147..69349 - 1203 WP_000976353 conjugal transfer protein TraF -
N6N74_RS24295 (N6N74_24320) 69435..70259 - 825 WP_001545755 conjugal transfer protein TraE traE
N6N74_RS24300 (N6N74_24325) 70410..71564 - 1155 WP_001139958 tyrosine-type recombinase/integrase -
N6N74_RS24305 (N6N74_24330) 71563..71880 + 318 WP_001558195 hypothetical protein -
N6N74_RS24310 (N6N74_24335) 73085..73333 + 249 WP_001349157 hypothetical protein -
N6N74_RS24315 (N6N74_24340) 73330..74622 - 1293 WP_001390622 shufflon system plasmid conjugative transfer pilus tip adhesin PilV -
N6N74_RS24320 (N6N74_24345) 74622..75278 - 657 WP_001193549 A24 family peptidase -
N6N74_RS24325 (N6N74_24350) 75263..75823 - 561 WP_000014005 lytic transglycosylase domain-containing protein virB1
N6N74_RS24330 (N6N74_24355) 75833..76447 - 615 WP_000908227 type 4 pilus major pilin -
N6N74_RS24335 (N6N74_24360) 76464..77549 - 1086 WP_001208802 type II secretion system F family protein -
N6N74_RS24340 (N6N74_24365) 77562..79115 - 1554 WP_001545756 GspE/PulE family protein virB11
N6N74_RS24345 (N6N74_24370) 79126..79578 - 453 WP_001247333 type IV pilus biogenesis protein PilP -
N6N74_RS24350 (N6N74_24375) 79565..80860 - 1296 WP_001545757 type 4b pilus protein PilO2 -
N6N74_RS24355 (N6N74_24380) 80853..82535 - 1683 WP_000748143 PilN family type IVB pilus formation outer membrane protein -
N6N74_RS24360 (N6N74_24385) 82549..82986 - 438 WP_001545758 type IV pilus biogenesis protein PilM -
N6N74_RS24365 (N6N74_24390) 82986..84053 - 1068 WP_024144162 type IV pilus biogenesis lipoprotein PilL -

Host bacterium

ID   21590 GenBank   NZ_CP110262
Plasmid name   pKBSW16i-B Incompatibility group   IncI1
Plasmid size   89449 bp Coordinate of oriT [Strand]   33690..33778 [-]
Host baterium   Escherichia albertii strain KBSW16i

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -