Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 121156 |
| Name | oriT_pYUSHP29-1  |
| Organism | Leclercia adecarboxylata strain SH19PE29 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP087281 (10426..10524 [-], 99 nt) |
| oriT length | 99 nt |
| IRs (inverted repeats) | 77..82, 89..94 (AAAAAA..TTTTTT) 77..82, 88..93 (AAAAAA..TTTTTT) 31..38, 41..48 (AGCGTGAT..ATCACGCT) 17..23, 35..41 (TAAATCA..TGATTTA) |
| Location of nic site | 59..60 |
| Conserved sequence flanking the nic site |
GGTGTATAGC |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 99 nt
>oriT_pYUSHP29-1
TTTGTTTTTTTTCTTTTAAATCAGTGCGATAGCGTGATTTATCACGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTTGGTAG
TTTGTTTTTTTTCTTTTAAATCAGTGCGATAGCGTGATTTATCACGCTGCGTTAGGTGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTTGGTAG
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 13356 | GenBank | WP_000130000 |
| Name | Replic_Relax_LO739_RS23130_pYUSHP29-1 |
UniProt ID | R4WML4 |
| Length | 101 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 101 a.a. Molecular weight: 11477.11 Da Isoelectric Point: 7.5204
>WP_000130000.1 MULTISPECIES: PadR family transcriptional regulator [Pseudomonadota]
MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGTVYPMLHGLEKKGYLTSRHERTGRRER
RVYDITEQGRTALADAKTKVKELFGELVEGG
MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGTVYPMLHGLEKKGYLTSRHERTGRRER
RVYDITEQGRTALADAKTKVKELFGELVEGG
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | R4WML4 |
Host bacterium
| ID | 21584 | GenBank | NZ_CP087281 |
| Plasmid name | pYUSHP29-1 | Incompatibility group | IncR |
| Plasmid size | 76208 bp | Coordinate of oriT [Strand] | 10426..10524 [-] |
| Host baterium | Leclercia adecarboxylata strain SH19PE29 |
Cargo genes
| Drug resistance gene | tet(A), qnrS1, dfrA14, aac(6')-Ib-cr, blaOXA-1, catB3, ARR-3, qacE, sul1, mph(A), mph(E), msr(E), floR, aph(3')-Ia, aph(6)-Id, aph(3'')-Ib |
| Virulence gene | - |
| Metal resistance gene | merE, merD, merA, merP, merT, merR |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |