Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 121113 |
| Name | oriT_pKO200862_1_OXA48  |
| Organism | Klebsiella pasteurii strain KO200862 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_CP143096 (31960..32065 [+], 106 nt) |
| oriT length | 106 nt |
| IRs (inverted repeats) | 30..35, 41..46 (CCGCCG..CGGCGG) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 106 nt
>oriT_pKO200862_1_OXA48
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
AGTACGGGACAAGATGTGTTTTTGGAGTACCGCCGACACGCGGCGGCCGTCCAAAAACGTCTTGGTCAGGGCAAGCCCCGACACCCCCTAACGAGGTTAGCTATCT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 13330 | GenBank | WP_004187323 |
| Name | traI_VZW71_RS28015_pKO200862_1_OXA48 |
UniProt ID | A0A3U8KUL3 |
| Length | 659 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 659 a.a. Molecular weight: 75179.89 Da Isoelectric Point: 9.9948
>WP_004187323.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacterales]
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
MVIPKIIEGRRDKKSSFGQLIKYMADKPSQELTDTVQPTPETALAVKSDMFEGLNNYLTRKQKVISTPVD
VEPGVQRVVVGDVTCQYNTFSLDGAAQEMNSVSQQSTRCKDPVMHYVLSWPDYEKPNDDQVFDSVKFTLA
SMGMSDHQYVAAIHRDTDNLHVHVAVNRINPQTYKAASSSFTKDTLHQACRLLELKNGWSHSNGAYVVND
RQQIVRNPHSKKERGNWRSLDRINKMENKEGVETLYRYIVGDEQVGGSRQNLIHVSAGLREAKSWDDVHK
TFADIGLRVEKAQGKKGYVITHEHQNQKTAVKASLVFNKAQYTLKSMEERFGEYQPSHIEPAKVSVFKTA
YTPGAYRRDANKRLQRKIERAEERMLLKGRYRAYRNNLPIYSPDKDRIADEYRKIAQHTRLVKNNVRHSV
SDPHTRKLMYNLAEFKRLQAVANLRLSLREERNGFRAANPRLSYREWVEQEALKGDKAALSQMRGFAYSS
RKKEKYKQQLVEQIGFNRTFNAITSHDRDDVAVMASARHGVKPRLLKDGTVIFERDGKPVAADRGHIVLT
ESNGIDKEKTADLAIALTIAGKAKSVRVDGDGEFKELCCNRIVDAAVNHNHPVAQGITFTDAAQQAYAQN
EKHRLIREQNNSKNEMQFRSESDDKFNPK
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3U8KUL3 |
T4CP
| ID | 15618 | GenBank | WP_004206885 |
| Name | t4cp2_VZW71_RS27790_pKO200862_1_OXA48 |
UniProt ID | _ |
| Length | 695 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 695 a.a. Molecular weight: 79538.91 Da Isoelectric Point: 4.8317
>WP_004206885.1 MULTISPECIES: conjugal transfer protein TrbC [Enterobacterales]
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
MQQESVDSKKVLRPDGSFMEWFLTPNVQFGLLAILTVLGLFLPFTMLLSILILPPLMVAFTDRKFRPPLR
MPKDCEMFDDTLTTETQAEYRLGPIRIPHKVRERKKAKGILYVGYERGRLFGRELWLNMTDLLRHMVFFG
TTGSGKTETFYGFIVNFLLWCRGYCLSDGKADNKLAFATWSLARRFGREDDYYVLNLLTGSIDRFVNLVK
QESIPAQSNSVNLFSVAPPTFIIQLMESMLPQVGGDSAQWQDTAKAMMSALINALCYKRARGELLLSQRT
IQKNMSLPAMAALYVEAKKNGWHSEGYAALESYLENTPGFLLANAEYPETWEARAFEQHNYMSRQFLKTL
SLFNETYGHVFPEDSGDIRMDDIFHNDRILIVMIPSLELSRGEAATLGRLYVTLQRMTISKDLGYQLEGK
KEEVLLTHALNNQAPYGLIYDELGQYFTSGMDTLSAQMRSLEKMGVFSSQDHPSLARGANGEVDSLIANT
RVKYFESIEDRKTFEILRETVGQDYYSELSGQEAEHGTFSKTVREGDLYQIREKDRVNIRELRKQTEGQG
VISFQDALVRSAAFYIPDNEKFSSELPMRINRFIEVLPPSPATLYSIYPERKDDELAETNPDAMRFPPIK
LFGYDKAANSLLEKIEVFYELQCGAISPEEMSVCLFELFAEWLDGTETDDVLYHQEDDLFPMIEM
Protein domains
No domain identified.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 34861..52398
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| VZW71_RS27995 (VZW71_28000) | 29945..30256 | + | 312 | WP_004187333 | hypothetical protein | - |
| VZW71_RS28000 (VZW71_28005) | 30389..30925 | + | 537 | WP_004187332 | hypothetical protein | - |
| VZW71_RS28005 (VZW71_28010) | 31427..31822 | - | 396 | WP_019725163 | hypothetical protein | - |
| VZW71_RS28010 (VZW71_28015) | 31857..32384 | + | 528 | WP_172740549 | plasmid mobilization protein MobA | - |
| VZW71_RS28015 (VZW71_28020) | 32371..34350 | + | 1980 | WP_004187323 | TraI/MobA(P) family conjugative relaxase | - |
| VZW71_RS28020 (VZW71_28025) | 34364..34864 | + | 501 | WP_004187320 | DotD/TraH family lipoprotein | - |
| VZW71_RS28025 (VZW71_28030) | 34861..35157 | + | 297 | WP_329523385 | type IV secretory system conjugative DNA transfer family protein | traI |
| VZW71_RS28030 (VZW71_28035) | 35211..35312 | + | 102 | WP_264766464 | hypothetical protein | traK |
| VZW71_RS28035 (VZW71_28040) | 35337..38774 | + | 3438 | WP_015586048 | LPD7 domain-containing protein | - |
| VZW71_RS28040 (VZW71_28045) | 38740..39252 | + | 513 | WP_011091071 | hypothetical protein | traL |
| VZW71_RS28045 (VZW71_28050) | 39253..39465 | - | 213 | WP_305953721 | Hha/YmoA family nucleoid-associated regulatory protein | - |
| VZW71_RS28050 (VZW71_28055) | 39437..39847 | - | 411 | WP_004187465 | H-NS family nucleoid-associated regulatory protein | - |
| VZW71_RS28055 (VZW71_28060) | 39915..40628 | + | 714 | WP_004187467 | DotI/IcmL family type IV secretion protein | traM |
| VZW71_RS28060 (VZW71_28065) | 40637..41788 | + | 1152 | WP_004187471 | DotH/IcmK family type IV secretion protein | traN |
| VZW71_RS28065 (VZW71_28070) | 41800..43149 | + | 1350 | WP_004187474 | conjugal transfer protein TraO | traO |
| VZW71_RS28070 (VZW71_28075) | 43161..43865 | + | 705 | WP_015060002 | conjugal transfer protein TraP | traP |
| VZW71_RS28075 (VZW71_28080) | 43889..44419 | + | 531 | WP_004187478 | conjugal transfer protein TraQ | traQ |
| VZW71_RS28080 (VZW71_28085) | 44436..44825 | + | 390 | WP_004187479 | DUF6750 family protein | traR |
| VZW71_RS28085 (VZW71_28090) | 44871..45365 | + | 495 | WP_004187480 | hypothetical protein | - |
| VZW71_RS28090 (VZW71_28095) | 45362..48412 | + | 3051 | WP_004187482 | hypothetical protein | traU |
| VZW71_RS28095 (VZW71_28100) | 48409..49617 | + | 1209 | WP_011091082 | conjugal transfer protein TraW | traW |
| VZW71_RS28100 (VZW71_28105) | 49614..50210 | + | 597 | WP_015060003 | hypothetical protein | - |
| VZW71_RS28105 (VZW71_28110) | 50203..52398 | + | 2196 | WP_015062834 | DotA/TraY family protein | traY |
| VZW71_RS28110 (VZW71_28115) | 52400..53053 | + | 654 | WP_015060005 | hypothetical protein | - |
| VZW71_RS28115 (VZW71_28120) | 53134..53364 | + | 231 | WP_011091085 | IncL/M type plasmid replication protein RepC | - |
Host bacterium
| ID | 21541 | GenBank | NZ_CP143096 |
| Plasmid name | pKO200862_1_OXA48 | Incompatibility group | IncL/M |
| Plasmid size | 53659 bp | Coordinate of oriT [Strand] | 31960..32065 [+] |
| Host baterium | Klebsiella pasteurii strain KO200862 |
Cargo genes
| Drug resistance gene | blaOXA-48 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |