Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   121023
Name   oriT_pGENC253 in_silico
Organism   Enterobacter hormaechei subsp. xiangfangensis strain GENC253
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NZ_CP061494 (208129..208383 [-], 255 nt)
oriT length   255 nt
IRs (inverted repeats)      153..158, 160..165  (AAAAGT..ACTTTT)
 122..130, 135..143  (TTAAGGCTT..AAGCCTTAA)
 49..55, 60..66  (GAATTTT..AAAATTC)
Location of nic site      78..79
Conserved sequence flanking the
  nic site  
 
 TTTGGTTAAA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 255 nt

>oriT_pGENC253
GGATTTAGGTTTTTTTTTAATCGCTTCACATTTCGTTAGCATGCGCAGGAATTTTTGATAAAATTCTGGTTAGTTTGGTTAAAAAGTGTTACAAGTAAGCGGTGTGGTTGAAGGGATAGATTTAAGGCTTATTCAAGCCTTAAGAAAATACTAAAAGTTACTTTTCACCCTACCGAACACCTAACAAAAAATCCATGTTGAAGATTTGAACAATTGTAATGGCGCAAGGACAATCAGCACATGTCAGAATCTGAT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   13269 GenBank   WP_328767293
Name   mobH_IN396_RS01150_pGENC253 insolico UniProt ID   _
Length   956 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 956 a.a.        Molecular weight: 107314.81 Da        Isoelectric Point: 4.5096

>WP_328767293.1 MobH family relaxase [Enterobacter hormaechei]
MNFRALYLCIKRILGIFSSQENDATSVMIEDISSLSPFAQILGDQKYTVPDHPNPEVLKFIEYPTRPTGI
QTFNEQSILSLYREKLHSISMMLAISDSDIRDDAYTFTNLVLKPLVEYVRWIHLLPASENHHHNGIGGLL
SHSLEVAILSLKNAHHSELRPIGYQDEEVVRRKVYLYAAFICGLVHDAGKVYDLDIVSLNLASPIIWTPS
SQSLLDWARENDVVEYEIHWRKRIHNQHNIWSSVFLERILNPVCLAFLDRVNKERVYSKMITALNVYTDG
NDFLSKCVRTADFYSTGTDLNVLRDPIMGLRSNDAAARAISTIKHNFTSININNYNAKPMHIIIVNGEVY
LNENAFLDFVLNDFELHKYNFPQGEAGKTVLVESLVQRGYVEPYDDERVVHYFIPGIYSENEISNIFRNG
IGKLEFYNLLKLRWIGLIFDSYKIPDSVPGLFSVNANKDFIYIDEQKTVTEYRRPVPGRDVITKITDTVE
TAVLKVNDLGRSSASIDVDIHSKKNEGSSDDFEKKAESDNEIDNDTQIVKSEGEEAADPVIPDIEESEDE
SAKDTESHVLVNQLHELLLSAPLSNDYIVCVDAVPYLNIDTTMALLPGLDEKAFSEEPYFQLTFREGSLD
GMWIVRDIDDLRLVQLGDNCAGFQLTYHEPRRPTTLKSLFNTSMYQALVINDESSVENSAPRPKQTLELP
PPRVNAVEEHSGDVEYHGTDSASATGPLKTEAVEYEHYQHLFEKEDEEHEIIDYTDFSQLSVSRPEVGSC
ATSSSVHNEKLLSEPSELPELNREQNADPQGTNERSMDVSVGQENSEPDTEGNCPPPAEVVYSQTEAAAT
SVMASEEPALPPVLEESNGEHAPTDAKGHHLSPALARLFAPTAPVEKQNPKRNRNKSSDKAEVQKPASPV
SGHNLNSKVFASTESDQNGEFSLISEGDVTELEFVEIALGKVRTSS

  Protein domains


Predicted by InterproScan.

(106-302)


  Protein structure



No available structure.




T4CP


ID   15551 GenBank   WP_001284073
Name   traD_IN396_RS01165_pGENC253 insolico UniProt ID   _
Length   694 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 694 a.a.        Molecular weight: 78123.91 Da        Isoelectric Point: 8.0177

>WP_001284073.1 MULTISPECIES: conjugative transfer system coupling protein TraD [Enterobacterales]
MSDSKRTNLHAQENFYRPILEYRSASILLICSVSMLYMGLSSDGLDIAPIVLFTSILLFLLCLYRCKTAA
PFLMAHWRVFKRHFMFVSLDSLRVINKSNFFSNERKYRQLVQDYQNKNKDIPERKSYFCDGFEWGPEHAD
RAYQIANLSSDKREIELPFVFNPIKRHFDAMARKMGGSNAIFAVERREPIFVTEDNWFGHTLITGNVGTG
KTVLQRLLSISMLHLGHVVVVIDPKNDAEWRESLMEEAKTLGLPFYKFHPGQPASSVCIDVCNTYTNVSD
LTSRLLSLVTVPGEVNPFVQYAKALVSNVISGLSYIEKKPSIYLIHKNMKSHMSIVNLTVKVMESCYARY
YGYDVWTEKVKYVANDTLPVRFKRLAEWFTAHFMNYEGSEQIDWLDTVSQLIDYSMSDPEHMAKMTAGIM
PVFDMLIEKPLNELLSPNPNSVSSREIVTSEGMFSTGGVLYISLDGLSNPDTAAAISQLIMSDLTSCAGS
RYNAQDGDMSANSRISIFVDEAHSAINNPMINLLAQGRAAKIALFICTQTISDFIAAASVETANRITGLC
NNYISLRVNDTPTQTLVVENFGKSAISTNMVTYTTGSETSLPHNNFSGSISERKQTTLEESIPKDLLGQV
PMFHIVARLQDGRKVVGQIPIAVAEKQMKPNTTLSEMLFKKAGKVTLRQNLDIKNLNKFLRKLH

  Protein domains


Predicted by InterproScan.

(406-609)

(199-249)

  Protein structure



No available structure.




T4SS


T4SS were predicted by using oriTfinder2.

Region 1: 2396..11636

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IN396_RS00005 1..876 + 876 WP_000594611 RepB family plasmid replication initiator protein -
IN396_RS00010 2025..2378 + 354 WP_000423602 hypothetical protein -
IN396_RS00015 2396..2746 + 351 WP_001447770 type IV conjugative transfer system protein TraL traL
IN396_RS00020 2758..3546 + 789 WP_000783153 TraE/TraK family type IV conjugative transfer system protein traE
IN396_RS00025 3546..4817 + 1272 WP_000592092 type-F conjugative transfer system secretin TraK traK
IN396_RS00030 4819..5280 + 462 WP_000521242 plasmid transfer protein HtdO -
IN396_RS00035 5270..6625 + 1356 WP_000351841 IncHI-type conjugal transfer protein TrhB traB
IN396_RS00040 6633..7115 + 483 WP_000377632 hypothetical protein -
IN396_RS00045 7234..7986 + 753 WP_182911723 protein-disulfide isomerase HtdT -
IN396_RS00050 7996..8946 + 951 WP_001022588 IncHI-type conjugal transfer lipoprotein TrhV traV
IN396_RS00055 8955..11636 + 2682 WP_000387412 TraC family protein virb4


Host bacterium


ID   21452 GenBank   NZ_CP061494
Plasmid name   pGENC253 Incompatibility group   IncHI2A
Plasmid size   306188 bp Coordinate of oriT [Strand]   208129..208383 [-]
Host baterium   Enterobacter hormaechei subsp. xiangfangensis strain GENC253

Cargo genes


Drug resistance gene   aac(6')-Ib-cr, blaOXA-1, aac(3)-IIa, blaCTX-M-15, tet(A), dfrA14, qnrB1, sul2, aph(3'')-Ib, aph(6)-Id, blaTEM-1B
Virulence gene   -
Metal resistance gene   terW, terZ, terA, terB, terC, terD, terE, merE, merD, merB, merR2, arsC, arsB, arsR, arsH
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -