Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 121019 |
| Name | oriT_pLAU_ENC1  |
| Organism | Enterobacter hormaechei subsp. xiangfangensis strain ST114 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | NZ_MN688131 (51552..51633 [+], 82 nt) |
| oriT length | 82 nt |
| IRs (inverted repeats) | 27..32, 35..40 (GCCACC..GGTGGC) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 82 nt
>oriT_pLAU_ENC1
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC
CGGGACAGGATATGTTTTGTATTACCGCCACCACGGTGGCAGCAATCCAAAACTCCTGGTCAGGGCGAAGCCCCGACACCCC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 13265 | GenBank | WP_015632446 |
| Name | traI_H5G65_RS00320_pLAU_ENC1 |
UniProt ID | R4WBX2 |
| Length | 642 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 642 a.a. Molecular weight: 74631.63 Da Isoelectric Point: 9.3060
>WP_015632446.1 MULTISPECIES: TraI/MobA(P) family conjugative relaxase [Enterobacteriaceae]
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDQRFRRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVTCQHNLMSVETAAMEMNSIAMQNLHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNLVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNTMFREKNASWEKLHQVMNDAGLML
EPSGKGLVVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSSRSAEMVKQSVQTPYSDRLHVRDRGA
RAERRQARAEARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIELKAERRKLYDEGRMRPLTYRAWTEQQALHGDQAALSQLRGWAYRQNRKNRTPQASDSI
IFCAPADDTSLIKADGYDARLYRDGAVVYSRHGQDAIIDRGDRVEVINPYDNGDLNTRIAVDMVAWKSGE
RIEFSRDGSFAYAAGDAVAAHNLHHRDSILVPSNREQYDYTREKFNQLKGRYTDREREAVRQQYDNRYYD
DVRHNDDNSYKP
MNAIIPPKRRDRKSSFVQLVAYVSVRDDVPLKDELKEDQRFRRPSRSRQAIFDRLVDYMNRSEGTIIEQI
SDMTPEGDIRCSVDGVTCQHNLMSVETAAMEMNSIAMQNLHVKDAVYHYILSWQEDENPSDDQVFDSVKH
SLKRLGMEEHQYVAAIHRDTDNLHVHIAANRVHPVSYRAANVWNDADKLQRTCRELELKHGFKVDNGSWV
RDADNNLVRARKGYRNAPRSARQLEHFSDKESLHHFAVQHVRKPVNTMFREKNASWEKLHQVMNDAGLML
EPSGKGLVVRDVCGDEKLAVKSSRVHPGMTLSRLEPALGTFVPSSRSAEMVKQSVQTPYSDRLHVRDRGA
RAERRQARAEARIDLRERYEKYRNAWKKPDLHAAERFRAVSAAFKEQKTHIRDNEKDAHMRKLMYHIVEF
EREKSMAALRIELKAERRKLYDEGRMRPLTYRAWTEQQALHGDQAALSQLRGWAYRQNRKNRTPQASDSI
IFCAPADDTSLIKADGYDARLYRDGAVVYSRHGQDAIIDRGDRVEVINPYDNGDLNTRIAVDMVAWKSGE
RIEFSRDGSFAYAAGDAVAAHNLHHRDSILVPSNREQYDYTREKFNQLKGRYTDREREAVRQQYDNRYYD
DVRHNDDNSYKP
Protein domains
Predicted by InterproScan.
Protein structure
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | R4WBX2 |
T4CP
| ID | 15547 | GenBank | WP_015632442 |
| Name | trbC_H5G65_RS00355_pLAU_ENC1 |
UniProt ID | _ |
| Length | 730 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 730 a.a. Molecular weight: 82688.74 Da Isoelectric Point: 5.8919
>WP_015632442.1 MULTISPECIES: F-type conjugative transfer protein TrbC [Enterobacteriaceae]
MQQKQKPVDAARLQRQVHQGMVEEMAYRTGPLLLAIIVIFGAAIVWPVLLWVAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLSRHMDNAAGVFYTGYERMLDGGREIWLRMDDLTRHLI
LLATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFIWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTITDPTETAEFAAKSAGMEYQAIQDSMEYRPGAISGSYADTGRVTVQEKSKLPFDELQTLN
QGEQATIFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLAHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPDAVQRATQLLSLAASVLTETRGRYSVEYPEP
EKLSVKKDEFEKYQHIHQSAVTQQADGFVY
MQQKQKPVDAARLQRQVHQGMVEEMAYRTGPLLLAIIVIFGAAIVWPVLLWVAIPVMLVWVPMFMLNEFR
MPARMPMDMDRLDPSTETMRPGKLLGFLPVTKLSRHMDNAAGVFYTGYERMLDGGREIWLRMDDLTRHLI
LLATTGAGKTEMLLGFVLNALCWAKGLIFSDGKAQNDVWFAVASLSRRFGREDDLRLLNYITGGQSRSQR
LLENDKSRQQSNNTNPFALAQETYITQLMDSMMPEAGNDRTWQEKAKGMNQVLTKALIYKSRKEQKVMSQ
RLIQEYLSLDKFAELYLQAEAEEWHEEIRTGLRNYLSTSVPGFDMSLITKPSEWSQEARNQHGYLTSQYN
RMLSLFSDTYGHVFSSGAGDIDLRDVVHNDRILVILIPALELSASEALTLGRLTTSQLAMILSQDLGERM
EGKAEDILVIKKFRDRFPFIWLADEVGSYYSDTIGQLATQVRSLGYALVLAGQDLQKLKTAVGDKLWTLL
GNMFTRIFGTITDPTETAEFAAKSAGMEYQAIQDSMEYRPGAISGSYADTGRVTVQEKSKLPFDELQTLN
QGEQATIFKGAVIRNNALYIADDDKITDKDVRINRFVEVEWPTYDSLAHLLPAKVRRDRPRPASINRIMA
IVKNDKIRHAPDSMIIQDGVLYAIADEAMYFDEVAPTPPDAVQRATQLLSLAASVLTETRGRYSVEYPEP
EKLSVKKDEFEKYQHIHQSAVTQQADGFVY
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 59494..81335
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| H5G65_RS00335 | 54554..54961 | + | 408 | WP_015632445 | transposase | - |
| H5G65_RS00340 | 54958..55307 | + | 350 | Protein_68 | IS66 family insertion sequence element accessory protein TnpB | - |
| H5G65_RS00345 | 55337..56926 | + | 1590 | WP_015632444 | IS66 family transposase | - |
| H5G65_RS00350 | 56949..57254 | - | 306 | WP_015632443 | phospholipase D-like domain-containing protein | - |
| H5G65_RS00355 | 57300..59492 | - | 2193 | WP_015632442 | F-type conjugative transfer protein TrbC | - |
| H5G65_RS00360 | 59494..60648 | - | 1155 | WP_015632441 | thioredoxin fold domain-containing protein | trbB |
| H5G65_RS00365 | 60596..62014 | - | 1419 | WP_015632440 | hypothetical protein | trbA |
| H5G65_RS00370 | 62116..62739 | - | 624 | WP_015632439 | plasmid IncI1-type surface exclusion protein ExcA | - |
| H5G65_RS00375 | 62805..64949 | - | 2145 | WP_015632438 | DotA/TraY family protein | traY |
| H5G65_RS00380 | 64991..65572 | - | 582 | WP_015632437 | conjugal transfer protein TraX | - |
| H5G65_RS00385 | 65569..66729 | - | 1161 | WP_032495747 | conjugal transfer protein TraW | traW |
| H5G65_RS00390 | 66776..69823 | - | 3048 | WP_015632435 | conjugal transfer protein | traU |
| H5G65_RS00395 | 69833..70396 | - | 564 | WP_032495746 | hypothetical protein | traT |
| H5G65_RS00400 | 70440..70841 | - | 402 | WP_015632433 | DUF6750 family protein | traR |
| H5G65_RS00405 | 70895..71428 | - | 534 | WP_015632432 | conjugal transfer protein TraQ | traQ |
| H5G65_RS00410 | 71438..72157 | - | 720 | WP_015632431 | conjugal transfer protein TraP | traP |
| H5G65_RS00415 | 72163..73503 | - | 1341 | WP_015632430 | conjugal transfer protein TraO | traO |
| H5G65_RS00420 | 73507..74610 | - | 1104 | WP_015632429 | DotH/IcmK family type IV secretion protein | traN |
| H5G65_RS00425 | 74621..75325 | - | 705 | WP_015632428 | DotI/IcmL/TraM family protein | traM |
| H5G65_RS00430 | 75361..75714 | - | 354 | WP_015632427 | hypothetical protein | traL |
| H5G65_RS00435 | 75748..79071 | - | 3324 | WP_032495745 | LPD7 domain-containing protein | - |
| H5G65_RS00440 | 79089..79355 | - | 267 | WP_032441926 | IcmT/TraK family protein | traK |
| H5G65_RS00445 | 79352..80539 | - | 1188 | WP_032495744 | plasmid transfer ATPase TraJ | virB11 |
| H5G65_RS00450 | 80517..81335 | - | 819 | WP_015632425 | type IV secretory system conjugative DNA transfer family protein | traI |
| H5G65_RS00455 | 81332..81799 | - | 468 | WP_015632424 | DotD/TraH family lipoprotein | - |
| H5G65_RS00460 | 81822..82301 | - | 480 | WP_015632423 | lytic transglycosylase domain-containing protein | - |
| H5G65_RS00465 | 82999..83310 | - | 312 | WP_015632421 | hypothetical protein | - |
| H5G65_RS00470 | 83344..83547 | - | 204 | WP_032495743 | hypothetical protein | - |
| H5G65_RS00475 | 83565..84098 | - | 534 | WP_015632420 | transcription termination/antitermination NusG family protein | - |
| H5G65_RS00480 | 84366..84944 | + | 579 | WP_015632419 | hypothetical protein | - |
| H5G65_RS00485 | 84911..85396 | + | 486 | WP_015632418 | hypothetical protein | - |
| H5G65_RS00490 | 85396..85782 | + | 387 | WP_015632417 | thioesterase family protein | - |
Host bacterium
| ID | 21448 | GenBank | NZ_MN688131 |
| Plasmid name | pLAU_ENC1 | Incompatibility group | IncFII |
| Plasmid size | 103199 bp | Coordinate of oriT [Strand] | 51552..51633 [+] |
| Host baterium | Enterobacter hormaechei subsp. xiangfangensis strain ST114 |
Cargo genes
| Drug resistance gene | blaNDM-1 |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |